Biomarkers for Ilex pubescens under anti-platelet activity-oriented isolation

Lin, Liping; Li, Ying; Zhang, Jichao; Lu, Ye

doi:10.1016/j.bse.2015.05.006

Cited by 4 publications

(1 citation statement)

References 17 publications

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“…Aquifoliaceae family comprises 400 species. Among them, Ilex pubescens (IP), under the Chinese name ‘Mao‐Dong‐Qing’, is an evergreen shrub, which is mainly distributed in southern regions of China (Lin, Ying, Zhang, & Lu, ). Its root is a well‐known traditional Chinese medicine herb commonly used for the treatment of cardiovascular disease and hypercholesterolemia (Zhou et al, ), and as a main ingredient in many formulae such as Mao‐Dong‐Qing injection, Mao‐Dong‐Qing tablet and Mao‐Dong‐Qing capsule.…”

Section: Introductionmentioning

confidence: 99%

An HPLC method for simultaneous quantitative determination of seven secoiridoid glucosides separated from the roots of Ilex pubescens

Zhang

Xiong

Zhao

et al. 2017

Biomedical Chromatography

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A simple and specific high-performance liquid chromatographic method has been developed and validated to simultaneously determine seven secoiridoid glucosides for the first time. Three of them were separated from the ethanolic extract of the roots of Ilex pubescens for the first time, namely nuezhenide A, ligusides B and oleonuezhenide. In quantitative analysis, all of the calibration curves showed good linear regression (r > 0.999) within the tested ranges, and the mean recoveries of three different concentrations ranged from 97.6 to 101.2%. The limit of detection and limit of quantification were <4.18 and 11.63 ng mL , respectively. The relative standard deviation for repeatability and the precision of seven analytes were <3.4 and 1.9%, respectively. The established method was successfully applied to simultaneous determination of seven secoiridoid glucosides in 11 batches of samples collected from different habitats in China.

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Section: Introductionmentioning

confidence: 99%