2022
DOI: 10.1002/jcla.24367
|View full text |Cite
|
Sign up to set email alerts
|

Biomarkers of connective tissue disease‐associated interstitial lung disease in bronchoalveolar lavage fluid: A label‐free mass spectrometry‐based relative quantification study

Abstract: Background:The pathogenesis of connective tissue disease-associated interstitial lung disease (CTD-ILD) is unclear. This study aims to identify differentially expressed proteins (DEPs) in CTD-ILD to determine the potential role of these DEPs that may play in the pathogenesis of CTD-ILD and to offer potential therapeutic targets.Methods: Bronchoalveolar lavage fluid (BALF) samples were collected from four patients with CTD-ILD and four patients without CTD-ILD. Label-free mass spectrometrybased relative quantif… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

0
6
0

Year Published

2022
2022
2023
2023

Publication Types

Select...
5
2

Relationship

0
7

Authors

Journals

citations
Cited by 8 publications
(6 citation statements)
references
References 53 publications
0
6
0
Order By: Relevance
“…Initially our protocol included a protein precipitation step, similar to other past protocols [ 28 , 30 ], to concentrate, and ostensibly eliminate contaminants from the BALF proteins. Methanol:chloroform precipitation was chosen as a means to phase separate potential lipid or surfactant contaminants from soluble proteins [ 30 ].…”
Section: Resultsmentioning
confidence: 99%
See 2 more Smart Citations
“…Initially our protocol included a protein precipitation step, similar to other past protocols [ 28 , 30 ], to concentrate, and ostensibly eliminate contaminants from the BALF proteins. Methanol:chloroform precipitation was chosen as a means to phase separate potential lipid or surfactant contaminants from soluble proteins [ 30 ].…”
Section: Resultsmentioning
confidence: 99%
“…Notably, a recent qualitative study using a contemporary high-resolution LC–MS/MS system and employing depletion of high abundance plasma proteins coupled with extensive, semi-preparative offline high pH HPLC fractionation has identified over 4000 proteins in BALF from lung cancer patients [ 27 ]. A recent quantitative study in BALF used semi-preparative offline high pH HPLC fractionation and label-free quantification to identify several thousand proteins, including those differentially expressed in diseases related to lung connective tissue [ 28 ]. Another recent study showed the potential for emerging data-independent acquisition (DIA) to quantify BALF proteins in lung cancer, where direct LC–MS analysis of tryptic digests from patient samples quantified over 600 proteins in these samples [ 29 ].…”
Section: Introductionmentioning
confidence: 99%
See 1 more Smart Citation
“…Only one study has described that the gene expression of PLBD1 is increased in psoriatic skin [ 91 ]. This gene was recently related to diverse types of cancer [ 92 , 93 , 94 ]. In 2020, a group demonstrated that PLBD1, together with eight other genes, can be a biomarker for the diagnosis of pancreatic adenocarcinoma [ 95 ].…”
Section: Discussionmentioning
confidence: 99%
“…Similar to the findings of Fietta et al using BALF of patients with SSc-ILD (19), calgranulin B was also increased in ILD patients with myositis overlap syndrome. Studies have shown that calgranulin B plays a role in endothelial inflammation and participates in the recruitment of white blood cells to the inflammatory site (55,56), and its expression is related to the inflammatory activity of systemic vasculitis (57). These findings suggest that there may be a common pathological pathway among different CTD-ILD subtypes, but the role of various proteins needs further classification research.…”
Section: Proteomics Studies In Idiopathic Inflammatory Myopathiesmentioning
confidence: 99%