Biomarkers of Diabetes Associated Oxidative Stress in Liver and Pancreas of Diabetic Mice after Administration of Lepidium Sativum Seed Extract

Abstract

Many phyto constituents like alkaloid, steroid, riboflavin, saponine have been recommended in the alterative system of medicine for the diabetes mellitus. Current research is now directed towards finding naturally occurring anti diabetic properties from plant origin. Lepidium sativum is an important herbal plant and its leaves and seeds have been used in various ailments and as a health tonic. The purpose of this study was to examine the anti-lipofuschinogenic activity of Lepidium sativum seed extract (LSE).For present study adult albino male mice (Mus musculus L) were divided into three group's viz. i. Control Group: male mice were given intraperitoneal injection of 0.5mlcitrate buffer pH 4.5ii. Diabetic Group-Mice were fed high fat diet (HFD) for two weeks and then injected with multi low dose of STZ (40mg/kg body weight) intraperitoneally (IP); in citrate buffer; pH 4.5 iii. Recovery group-Diabetic mice were given LSE (at a dose 200mg/kg body wt., dissolved in 0.5ml distilled water) orally for 28 days. After completion of treatment body weight, pancreas and liver weight, level of oxidative stress marker i.e. lipid peroxidation and fluorescence was studied. Result showed decrease in body weight and gland weight in diabetic group and increase in recovery group. The end product of lipid peroxidation malondialdehyde (MDA) and fluorescence product were elevated in diabetic group and after LSE administration the both the parameters reduced significantly. The results showed an anti-lipofuschinogenic activity of Lepidium sativum seed extract.