Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokines

El-Deeb, Wael; El-Bahr, Sabry M.

doi:10.24099/vet.arhiv.160126c

Cited by 31 publications

(25 citation statements)

References 36 publications

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“…Nowadays, APPs have become important diagnostic biomarkers in human medicine, as well as, in veterinary diagnostics [ 35 ]. Interest in APPs as potential biomarkers in veterinary medicine involves the evaluation of their concentration and modifications, as well as, their interaction as a part of the host response [ 36 - 39 ].…”

Section: Introductionmentioning

confidence: 99%

Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle

Hussein¹,

El-Razik²,

Gomaa³

et al. 2018

Vet World

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Background and Aim:Mastitis is one of the most vital noteworthy monetary risks to dairy ranchers and affects reproductive performance in dairy cattle. However, subclinical mastitis (SCM) negatively affects milk quality and quantity and associated with economic losses as clinical mastitis. It is recognizable only by additional testing. Somatic cell count (SCC) is currently used worldwide for the screening of intramammary infection (IMI) infections. However, somatic cells (SC) are affected by numerous factors and not always correlate with infection of the udder. Therefore, the aim of the present study was to evaluate the milk amyloid A (MAA) in the milk of normal and SCM cows and compare the sensitivity of both MAA secretion and SCC in response to mammary gland bacterial infection.Materials and Methods:A total of 272 quarter milk samples collected from 68 Friesian cows after clinical examination for detection of clinical mastitis were employed in this study. All quarter milk samples (272) were subjected to bacteriological examination, while SCs were assessed in samples (220). Following SCC estimation and bacteriological examination, the apparently normal quarter milk samples were categorized into 7 groups and MAA concentration was estimated in normal and subclinical mastitic milk samples.Results:Prevalence of clinical mastitis was 19.12 % (52 quarters), while 80.88 % (220 quarters) were clinically healthy with normal milk secretion. Of those 220 clinically healthy quarter milk samples, 72 (32.73%) showed SCM as detected by SCC (SCC ≥500,000 cells/ml). The most prevalent bacteria detected in this study were streptococci (48.53%), Staphylococcus aureus (29.41%), Escherichia coli (36.76%), and coagulase-negative staphylococci (11.76%). Results of MAA estimation revealed a strong correlation between MAA secretion level and SCC in agreement with the bacteriological examination. Interestingly, there was a prompt increase in MAA concentration in Group III (G III) (group of milk samples had SCC ≤200,000 cells/ml and bacteriologically positive) than Group I (G I) (group of milk samples with SCC ≤500,000 cells/ml and bacteriologically negative), as MAA concentration in G III was about 4 times its concentration in G I.Conclusion:Our study provides a strong evidence for the significance of MAA measurement in milk during SCM, and MAA is more sensitive to IMI than SCC. This can be attributed to rapid and sensitive marker of inflammation. The advantage of MAA over other diagnostic markers of SCM is attributed the minute or even undetectable level of MAA in the milk of healthy animals, it is not influenced by factors other than mastitis, and could be estimated in preserved samples. Therefore, we recommend that estimation of MAA concentration in milk is a more useful diagnostic tool than SCC to detect SCM and to monitor the udder health in dairy cattle.

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Section: Introductionmentioning

confidence: 99%

Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle

Hussein¹,

El-Razik²,

Gomaa³

et al. 2018

Vet World

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“…Chalmeh et al [169] observed in sheep, a rapid increase of SAA values during experimentally induced endotoxaemia by lipopolysaccharide from Escherichia coli. Another study conducted by El-Deeb [170] showed an increase in the concentrations of SAA in ewes with pregnancy toxemia. Marked increase of SAA concentrations was recorded also in sheep following experimental infestation with Psoroptes ovis [171].…”

Section: Serum Amyloid Amentioning

confidence: 91%

The Use of Serum Proteins in the Laboratory Diagnosis of Health Disorders in Ruminants

Tóthová¹,

Mihajlovičová²,

Nagy³

2018

Ruminants - The Husbandry, Economic and Health Aspects

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Although hundreds of proteins exist in blood serum, little is known about the precise composition and entire set of serum proteins in different ruminant species. Under physiological conditions, the production of serum proteins is closely regulated, but alterations in the serum protein pattern may occur in a wide range of diseases and health disorders. During the last several years, substantial progress was seen in the application of serum protein analyses for diagnostic purposes. The serum protein profile is mostly evaluated by serum protein electrophoresis, which allows the identification of protein fractions, each being composed of several individual proteins with similar electrophoretic mobility. Many disease processes can cause changes in the concentrations of serum proteins. Therefore, the determination of their concentrations and the evaluation of changes in their concentrations during the disease process may provide important diagnostic information for assessing the health state. Despite this usefulness, the evaluation of serum protein pattern is still relatively a less frequently used laboratory diagnostic technique in ruminant medicine. Thus, the usefulness of serum proteins in the diagnosis of health disorders and the possible clinical application of the results of the electrophoretic separation of serum proteins in ruminants will be reviewed in this chapter.

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“…Bei erhöhten NEFA-und BHB-Konzentrationen in Blut und Milch war auch die Hp-Konzentration im Blut erhöht. Dies entspricht den Ergebnissen anderer Studien und spricht dafür, dass dieses Akute-Phase-Protein als Biomarker für erhöhte Stoffwechselbelastungen bei Milchkühen in der Transitphase herangezogen werden könnte und dass eine Stoffwechselstörung mit einer Entzündungsreaktion einhergeht [29] [30]. Abuajamieh et al [30] wiesen erhöhte Hp-Konzentrationen im Blut nach, noch bevor die Kühe klinische Symptome einer Ketose zeigten.…”

Section: Diskussion Der Ergebnisseunclassified

“…Aufgrund einer negativen Energiebilanz p. p. steigen die Lipomobilisation und gegebenenfalls die NEFA-und BHB-Konzentrationen im Blut an. Dies übt durch die Bildung von Triglyzeriden und proinflammatorischen Zytokinen einen oxidativen Stress auf die Leber aus, was zu einem Anstieg der Hp-Konzentration führt [1] [29]. Ein weiterer potenzieller Grund für erhöhte Hp-Konzentrationen bei abweichenden Stoffwechselparametern ist die geringere Futteraufnahme bei Tieren mit Stoffwechselstörungen, aus der eine beeinträchtigte Darmtätigkeit resultieren kann.…”

Section: Diskussion Der Ergebnisseunclassified

Nutzung der Haptoglobinkonzentration im Blutserum als Indikator im Tiergesundheitsmonitoring bei Milchkühen

Hajek

Reus

Gruber

et al. 2020

Tierarztl Prax Ausg G Grosstiere Nutztiere

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Zusammenfassung Ziel Untersuchung von Zusammenhängen zwischen der Haptoglobinkonzentration im Blut und dem Auftreten einer Ketose, ausgewählten klinischen Parametern und Lahmheit bei Milchkühen. Material und Methoden Die Datenerhebung fand in 39 bayerischen Milchviehbetrieben über 8 Monate statt. Bei den einbezogenen 712 Fleckvieh- und Braunviehkühen, deren Kalbung 10–30 Tage zurücklag, wurde eine klinische Untersuchung durchgeführt sowie eine Milch- und eine Blutprobe entnommen. Im Blut wurden die Konzentrationen von freien Fettsäuren (non-esterified fatty acids, NEFA), β-Hydroxybutyrat (BHB) und Haptoglobin (Hp) bestimmt, in der Milch die Milchinhaltsstoffe (Fett, Eiweiß, Harnstoff, Laktose, Azeton), BHB, NEFA und die somatische Zellzahl (SCC). Ergebnisse Signifikante Korrelationen ergaben sich zwischen erhöhten Hp-Konzentrationen einerseits und erhöhten NEFA-Konzentrationen in Blut und Milch (p < 0,001), erhöhter SCC (p < 0,001), Lahmheit (p < 0,001), vermindertem Laktosegehalt (p < 0,001) sowie vermindertem Proteingehalt der Milch (p = 0,001) andererseits. In den wärmeren Sommermonaten beprobte Tiere hatten signifikant höhere Hp-Konzentrationen (p < 0,001). Erstkalbinnen wiesen signifikant höhere Hp-Werte auf als multipare Tiere (p < 0,001). Durch die Einteilung der Tiere in 4 Cluster ließ sich ein Hp-Grenzwert bestimmen, der 0,18 mg/ml betrug. Kombiniert mit einem SCC-Grenzwert von 40 500 Zellen/ml Milch konnte der Großteil der subklinisch und klinisch auffälligen Tiere ermittelt werden. Schlussfolgerung und klinische Relevanz Die Messung der Hp-Konzentration im Blut stellt einen praktikablen Ansatz zur Unterstützung des Tiergesundheitsmonitorings im postpartalen Zeitraum dar. Zusammen mit der Auswertung der Milchmenge und der Milchinhaltsstoffe können Abweichungen von physiologischen Zuständen erkannt und betroffene Tiere zeitnah behandelt werden. Haptoglobin kann für die Bewertung des Gesundheitsstatus des Einzeltieres wie auch als Indikator für die Herdengesundheit genutzt werden.

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Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokines

Cited by 31 publications

References 36 publications

Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle

Milk amyloid A as a biomarker for diagnosis of subclinical mastitis in cattle

The Use of Serum Proteins in the Laboratory Diagnosis of Health Disorders in Ruminants

Nutzung der Haptoglobinkonzentration im Blutserum als Indikator im Tiergesundheitsmonitoring bei Milchkühen

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