Biomarkers of Myocardial Fibrosis

Jong, Sanne de; Veen, Toon A.B. van; Bakker, Jacques M.T. de; Vos, Marc A.; Rijen, Harold V.M. van

doi:10.1097/fjc.0b013e31821823d9

Cited by 94 publications

(86 citation statements)

References 80 publications

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“…In heart diseases, chronic neurohormonal stimulation can result in the deposition of interstitial connective tissue (i.e., reactive fibrosis) as well as myocardial necrosis followed by fibrous tissue formation (i.e., reparative fibrosis) 33, 34. A study in humans with dilated cardiomyopathy indicated that deposition of interstitial collagen may cause deterioration in ventricular function.…”

Section: Discussionmentioning

confidence: 99%

Pathologic Manifestations on Surgical Biopsy and Their Correlation with Clinical Indices in Dogs with Degenerative Mitral Valve Disease

Lee

Mizuno

et al. 2015

Veterinary Internal Medicne

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BackgroundEvaluation of myocardial function is clinically challenging in dogs with degenerative mitral valve disease (DMVD). Although myocardial dysfunction is caused by pathologic degeneration, histopathologic progression is poorly understood.ObjectivesTo characterize myocardial and pulmonary pathologic changes according to severity in dogs with naturally occurring DMVD, and to investigate whether or not pathologic degeneration is reflected by traditional clinical indices.AnimalsOne hundred and seventeen dogs with naturally occurring DMVD.MethodsProspective observational study. Biopsied left atrium (LA), left ventricle (LV), and lung were evaluated histologically, and an attempt was made to correlate pathologic findings with clinical indices.ResultsSevere myocardial changes were observed in all International Small Animal Cardiac Health Council classes. In the lung, heart failure cell levels were significantly increased in class III patients (P < .0001). In a paired comparison, the LA showed significantly more severe degeneration than the LV, including myocardial fatty replacement, immune cell infiltration, and interstitial fibrosis (P < .0001). In contrast, myocardial cells were more hypertrophied in the LV than in the LA (P < .0001). Left ventricular end‐diastolic dimension (LVEDd) was associated with fatty replacement (P = .033, R 2 = 0.584) and myocardial vacuolization (P = .003, R 2 = 0.588) in the LA.Conclusions and Clinical ImportanceIn DMVD, although severe pathologic changes may be evident even in early stages, there may be pathologic discrepancy between the LA and the LV. Myocardial degeneration may be reflected by clinical indices such as LVEDd and EF.

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Section: Discussionmentioning

confidence: 99%

Pathologic Manifestations on Surgical Biopsy and Their Correlation with Clinical Indices in Dogs with Degenerative Mitral Valve Disease

Lee

Mizuno

et al. 2015

Veterinary Internal Medicne

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“…Several lines of evidence indicate that the activation of hypertrophic markers, matrix metalloproteinases (MMPs), and proinflammatory cytokines play a central pathophysiological roles in the development of cardiac hypertrophy, fibrosis, and heart failure in the experimental animal models and humans (11,52,58,66). Previous studies have suggested that ANP/ NPRA signaling acts as a negative regulator of inflammation and hypertrophic growth (14,65,67,69).…”

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confidence: 99%

Retinoic acid and sodium butyrate suppress the cardiac expression of hypertrophic markers and proinflammatory mediators in Npr1 gene-disrupted haplotype mice

Umadevi

Kumar

Mani

et al. 2016

Physiological Genomics

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“…1 The extent of fibrosis and collagen scar is an important factor in the assessment of interventions aimed at treating cardiac diseases. 2 Hence an efficient and reliable measurement of collagen deposition in cardiac tissue is of great interest in the assessment of treatment strategies for cardiac pathologies. Because of several limitations and the variability experienced in staining techniques, we propose an alternative FT-IRIS methodology for detection and characterization of collagen deposition in cardiac tissue.…”

Section: Discussionmentioning

confidence: 99%

“…In cardiac tissue, this matrix supports, connects, and coordinates cardiac myocytes and the forces generated by them. 1,2 Several cardiac pathologies are associated with significant changes in the composition of the matrix. 1,2 Therefore, detection and quantitative measurement of these matrix changes play an important role in experimental assessment of novel treatment methods and drug delivery systems for treating cardiac diseases.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 Several cardiac pathologies are associated with significant changes in the composition of the matrix. 1,2 Therefore, detection and quantitative measurement of these matrix changes play an important role in experimental assessment of novel treatment methods and drug delivery systems for treating cardiac diseases. Adverse cardiac remodeling and cardiac fibrosis after myocardial infarction is one of the most commonly studied aspects of ECM alteration in cardiac tissue.…”

Section: Introductionmentioning

confidence: 99%

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Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction

et al. 2012

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Abstract. Myocardial infarction often leads to an increase in deposition of fibrillar collagen. Detection and characterization of this cardiac fibrosis is of great interest to investigators and clinicians. Motivated by the significant limitations of conventional staining techniques to visualize collagen deposition in cardiac tissue sections, we have developed a Fourier transform infrared imaging spectroscopy (FT-IRIS) methodology for collagen assessment. The infrared absorbance band centered at 1338 cm −1 , which arises from collagen amino acid side chain vibrations, was used to map collagen deposition across heart tissue sections of a rat model of myocardial infarction, and was compared to conventional staining techniques. Comparison of the size of the collagen scar in heart tissue sections as measured with this methodology and that of trichrome staining showed a strong correlation (R ¼ 0.93). A Pearson correlation model between local intensity values in FT-IRIS and immuno-histochemical staining of collagen type I also showed a strong correlation (R ¼ 0.86). We demonstrate that FT-IRIS methodology can be utilized to visualize cardiac collagen deposition. In addition, given that vibrational spectroscopic data on proteins reflect molecular features, it also has the potential to provide additional information about the molecular structure of cardiac extracellular matrix proteins and their alterations.

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Biomarkers of Myocardial Fibrosis

Cited by 94 publications

References 80 publications

Pathologic Manifestations on Surgical Biopsy and Their Correlation with Clinical Indices in Dogs with Degenerative Mitral Valve Disease

Pathologic Manifestations on Surgical Biopsy and Their Correlation with Clinical Indices in Dogs with Degenerative Mitral Valve Disease

Retinoic acid and sodium butyrate suppress the cardiac expression of hypertrophic markers and proinflammatory mediators in Npr1 gene-disrupted haplotype mice

Fourier transform infrared spectroscopic imaging of cardiac tissue to detect collagen deposition after myocardial infarction

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