Biomarkers of waterborne copper exposure in the Neotropical fish Prochilodus lineatus

Simonato, Juliana Delatim; Prodocimo, Maritana Mela; Doria, Halina Binde; Guiloski, Izonete Cristina; Randi, Marco Antônio Ferreira; Carvalho, Paulo S.M.; Meletti, Paulo César; Assis, Helena C. Silva de; Bianchini, Adalto; Martinez, Cláudia B. R.

doi:10.1016/j.aquatox.2015.11.012

Cited by 65 publications

(20 citation statements)

References 59 publications

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“…The results showed liver experienced the most damages compared to gill and muscle. This is also proved by previous studies performed on sh as done by (Simonato et al, 2016; Samantha et al, 2018) since liver acts as a depository organ for various pollutants. The level of GST response was higher compared to GR.…”

Section: Glutathione-dependent Enzymes Systemsupporting

confidence: 70%

Evaluation of Different Functional Antioxidant Groups and Protein Responses in Asian Seabass (Lates calcarifer Bloch, 1970) as an Early Indication of Fish Health in Aquaculture Farms.

Hassan

Jaafar

Yusoff

et al. 2020

Preprint

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The study was conducted to investigate the organ-specific antioxidants and protein damages responses in Lates calcarifer inhabiting different aquaculture farms that susceptible to different threat of pollutions. Enzymes at the front line of antioxidant system superoxidase dismutase (SOD) and catalase (CAT) evidenced to work together but respond differently in different body compartment. High SOD responses were followed with lower CAT responses observed in muscle (p < 0.05) with opposite responses exhibited by both gill and liver. The responses of SOD and CAT in muscle also showed a significant strong correlation to each other (Setiu Wetland: 0.91, Semerak: 0.79) (p < 0.01). The glutathione-dependent enzymes, glutathione-s-transferase (GST) and glutathione reductase (GR) in body compartments responded with a strong correlation to each other especially in muscle (Tumpat; muscle: 0.89, gill: 0.95 and liver: 0.54 (p < 0.01, p < 0.05), (Setiu Wetland; muscle: 0.72 (p < 0.01) and Semerak; muscle: 0.79 (p < 0.01). Opposite results were found for both protein damages biomarkers, thiols (-SH-) and carbonyl (-CH-) in comparison to biomarkers responses. In contrary to (-SH-) inconsistent results were observed for the (-CH-) with muscle found to be most oxidised. The responses of SOD, CAT, GST, GR, thiols and carbonyl were all computed into the same scale through Integrated Biomarker Score (IBR) to classify each aquaculture farms based on biomarkers responses. There was a slight variation in deviation score (A) between organs within the range of (5-7). However, Tumpat overall showed the highest IBR score and significantly higher (p < 0.05) than Setiu Wetland with a total score of combining responses score in all three organs (IBR: 84, muscle: 31, gill: 24, and liver: 29) followed by intermediate score in Semerak (IBR: 72, muscle: 23, gill: 23 and liver: 26) and Setiu Wetland (IBR: 59, muscle: 18, gill: 23 and liver: 18). These results indicated that the responses of antioxidant enzymes and protein damages in L.calcarifer from different organs are heterogeneous. Therefore, biomarkers should be selected based on their sequential groups in the antioxidant system for a better explanation of the oxidative stress evaluation in fish.

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Section: Glutathione-dependent Enzymes Systemsupporting

confidence: 70%

Evaluation of Different Functional Antioxidant Groups and Protein Responses in Asian Seabass (Lates calcarifer Bloch, 1970) as an Early Indication of Fish Health in Aquaculture Farms.

Hassan

Jaafar

Yusoff

et al. 2020

Preprint

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“…The monitoring of metals and pesticides in rivers surrounded by intensive industrial and agricultural activities is very important (Machado et al, 2016). Still, it is expressive the concern of Brazilian research with tannery (Rocha & Oliveira, 2017), copper (Simonato et al, 2016), heavy metals (Paula Filho et al, 2015) and microplastics (Santana et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Biomonitoring using comet assay in fish: a scientometric approach

T.F.M.¹,

Sampaio²,

Ghisi³

2024

Ecotoxicol. Environ. Contam.

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The goal of this paper is to analyze the knowledge development on biomonitoring using comet assay on fishes. Were selected 154 papers in the ISI Web of Science and PubMed databases. The comet assay was used by 24.7% of the papers with the purpose to evaluate genotoxicity of polluted water. Comet assay is many times associated with other techniques, offering good results to increase efficiency in environmental assessment or to evaluate genotoxicity of pollutants individually.

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“…Apparently MTs are involved in regulating the Cu as an essential metal, and the MT binding capacity to Cu has not been exceeded [42,43]. MT can also act as non-toxic metal reserves for metalloenzyme synthesis, protect against ionizing radiation, and play an important role as an antioxidant [44,45].…”

Section: Discussionmentioning

confidence: 99%

The Change of Metallothionein and Oxidative Response in Gills of the Oreochromis niloticus after Exposure to Copper

Ma’rifah

Saputri

Soegianto

et al. 2019

Animals

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In the present study, we investigated the effects of waterborne copper (Cu) on the levels of metallothionein (MT) and malondialdehyde (MDA), as well as activities of superoxide dismutase (SOD) and catalase (CAT) in gills of cichlid fish Oreochromis niloticus. The Cu concentrations in gills were measured using an atomic absorption spectrometer. The sandwich-ELISA was used to measure MT, SOD, CAT, and MDA. The Cu concentrations in gills of fish that were exposed to 1, 5, and 10 mg Cu/L were significantly increased at day 1 (D1), then gradually decreased starting from D2, and reaches the similar value with the controls at D5. A similar tendency has been observed in the MT levels in the gills. All of the Cu-exposed fish showed the highest level of MT on D1, and then decreased at D3 and a plateau at D4 and D5. The levels of SOD and CAT in gills in all Cu-exposed fish showed a similar pattern: increased significantly at D1, then gradually decreased starting from D2, and increased again at D4 and D5. The levels of MDA in gills of all Cu-exposed fish showed no significant difference. The indifference levels of MDA in gills of all Cu-exposed fish suggested the antioxidant defense systems (SOD and CAT) combined with the induction of MT were able to completely scavenge the increased ROS.

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Biomarkers of waterborne copper exposure in the Neotropical fish Prochilodus lineatus

Cited by 65 publications

References 59 publications

Evaluation of Different Functional Antioxidant Groups and Protein Responses in Asian Seabass (Lates calcarifer Bloch, 1970) as an Early Indication of Fish Health in Aquaculture Farms.

Evaluation of Different Functional Antioxidant Groups and Protein Responses in Asian Seabass (Lates calcarifer Bloch, 1970) as an Early Indication of Fish Health in Aquaculture Farms.

Biomonitoring using comet assay in fish: a scientometric approach

The Change of Metallothionein and Oxidative Response in Gills of the Oreochromis niloticus after Exposure to Copper

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