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Bacillus thuringiensis (Bt), an entomopathogenic bacterium, has been used as bioinsecticides for insect pest control worldwide. Consequently, the objective of this work was to evaluate the possible effects of commercial formulations of Bt products, Dipel and Xentari, on the survival and behavior of Africanized honey bees (Apis mellifera). Bioassays were performed on foragers and newly emerged (24-h-old) bees that received the products mixed in the food. Their survival and behavior were evaluated through the vertical displacement tests and the walk test, analyzed using software Bee-Move. Then, histological analysis of the mesenterium was performed. As control treatment was used sterile water. The honey bees’ survival was evaluated for between 1 and 144 h. No interference of B. thuringiensis, Dipel and Xentari, in the survival of Africanized honey bees were found. Only Xentari interfered with vertical displacement behavior of newly emerged (24-h-old) bees. Both the products tested were selective and safe for A. mellifera.

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The goal of this paper is to analyze the knowledge development on biomonitoring using comet assay on fishes. Were selected 154 papers in the ISI Web of Science and PubMed databases. The comet assay was used by 24.7% of the papers with the purpose to evaluate genotoxicity of polluted water. Comet assay is many times associated with other techniques, offering good results to increase efficiency in environmental assessment or to evaluate genotoxicity of pollutants individually.