Biomass-bound biocatalysts for biodiesel production: Tuning a lipolytic activity from Aspergillus niger MYA 135 by submerged fermentation using agro-industrial raw materials and waste products

Regner, Erika Lucía; Salvatierra, Hebe Natalia; Baigorí, Mario Domingo; Pera, Licia María

doi:10.1016/j.biombioe.2018.11.005

Cited by 21 publications

(8 citation statements)

References 50 publications

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“…Pseudomonas cepacia (Kumar et al, 2020), P. fluorescens (Ferrero et al, 2020), Thermomyces lanuginosus (Gumba et al, 2016), Candida rugose (Sánchez-Bayo et al, 2019), C. Antarctica (Sánchez-Bayo et al, 2019, Rhizopus sp. (Wahyuningsih et al, 2015), Aspergillus niger (Almyasheva et al, 2018;Regner et al, 2019), Trichoderma sp. especially T. reesei, Harzianum, Viride (Riwayati et al, 2012), actinobacteria or actinomycetes Streptomyces exfoliates and Nocardiopsis alba (Faturahman, 2019), Yeast (Deeba et al, 2020), Cladosporium tenuissimum (Saranya and Ramachandra, 2020).…”

Section: Resultsmentioning

confidence: 99%

Bioconversion of Black Soldier Fly (BSF) from Organic Waste Composting into Biodiesel Assisted by Whole Cell Microbial Lipase Biocatalyst through Direct Transesterification Process

Ningrum,

Zulaika,

Hanif S

et al. 2023

J. Presipitasi

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Black Soldier Fly or Hermetia Illucens (BSF) is known for its potential as a biological agent that helps in the process of natural conversion of organic waste. Resulting compost and a large number of BSF colonies, potentially to be use as a non-food raw material in bioenergy production. The Method is BSF conversion experiment by direct transesterification reaction using Lab-scale bioreactor in 100ml three bottle neck flasks, the solution mixture consists of BSF powder, immobilized whole cell biocatalyst, and solvent. The Result is although in appearance the structure of the test mixture solution did not show an oily character, but based on the viscosity and density test, the test solution had density value 0.81 g/cm3 and viscosity value 1,024 mm2 /s which are above the value of the viscosity and density of methanol but still below the value of the viscosity and density of both SNI and EN14214 biodiesel standard, this could be due to insufficient separation or reaction, imperfect condition, and impurities that are still present in biodiesel products. It can be concluded that a transesterification reaction has occurred in this trial, however further analysis and more experiments are required to definitely conclude the changes in biodiesel production.

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Section: Resultsmentioning

confidence: 99%

Bioconversion of Black Soldier Fly (BSF) from Organic Waste Composting into Biodiesel Assisted by Whole Cell Microbial Lipase Biocatalyst through Direct Transesterification Process

Ningrum,

Zulaika,

Hanif S

et al. 2023

J. Presipitasi

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“…Um cotejo dos resultados apresentados nesta seção com os de outros estudos que também avaliaram lipases ligadas ao micélio pela hidrólise de pNPP mostrou que, na maioria dos casos, a atividade hidrolítica das linhagens amazônicas testadas neste trabalho foi mais alta. Regner et al, 33 trabalhando com A. niger (linhagem MYA 135) e testando diversas formulações de meio preparadas a partir de matérias primas e resíduos agro-industriais, obtiveram aproximadamente 850 mU g -1 usando micélio lavado com água e acetona e centrifugado. Anteriormente, Colin et al, 34 trabalhando com a mesma linhagem de A. niger, mas cultivada em meio suplementado com óleo de oliva e Fe +3 , relataram atividade hidrolítica de aproximadamente 1.800 mU g -1 utilizando micélio úmido (lavado e centrifugado).…”

Section: Comparação Dos Resultados Obtidos Com a Biomassa Delipidadaunclassified

“…De fato, diversos estudos relacionados à síntese de ésteres e ao biodiesel empregaram lipases ligadas ao micélio deste gênero de fungo. 25,33,34,38,39 Romero et al 39 e Colin et al 34 avaliaram a atividade sintética das lipases ligadas ao micélio de A. niger MYA 135 pela transesterificação entre pNPP e etanol, mensurada pela liberação do pNP detectada a 405 nm. Usando micélio lavado superficialmente com acetona e centrifugado, Romero et al obtiveram como melhor resultado 69,4±9,4 mU g -1 .…”

Section: Avaliação Da Atividade Sintéticaunclassified

Avaliação Da Biomassa De Fungos Amazônicos Como Fonte De Lipases Para Biocatálise

Romano¹,

Santos²,

Louzada³

et al. 2020

Quím. Nova

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EVALUATION OF AMAZONIAN FUNGI BIOMASS AS A SOURCE OF LIPASES FOR BIOCATALYSIS. We evaluated the biomass of twenty Amazonian fungal isolates as a potential source of mycelium-bound lipases with hydrolytic, synthetic or enantioselective activity for biocatalysis application. We compared the hydrolytic activity of three biomass treatments (delipidated, non-delipidated and cultivated in medium without inducer). Delipidated biomass showed better results in the hydrolysis of p-nitrophenyl palmitate compared to the other two treatments for fifteen isolates. Delipidated biomass of six Aspergillus strains and UEA_115 strain showed a high synthesis activity of ethyl palmitate by transesterification in organic medium. Results were confirmed by spectrophotometry (410 nm) and gas chromatography. In this reaction, the isolate DPUA_1539 A. flavo-furcatis reached a maximum value of 668.5 ± 23.5 mU g-1. Enantioselective activity assays indicated that biomass-bound lipases from UEA_115 isolate (E = 3.58; ee s = 7 ± 0) and in particular from the DPUA_1539 A. flavo-furcatis isolate (E = 24.15; ee s = 91 ± 1) have the ability to discriminate enantiomers of the drug ibuprofen by ester synthesis, preferably with (R)-enantiomer. These results encourage further investigations of these fungi as potential lipase suppliers for biocatalytic processes such as biodiesel production and enantiopure drugs.

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“…Whole-cell biocatalysis has been successfully used in research on sustainable biodiesel production. The mycelium-bound lipases of mesophilic fungi have been of particular interest, such as those of Rhizopus chinensis [17], Aspergillus niger [9,10,18], A . nomius , A .…”

Section: Discussionmentioning

confidence: 99%

“…However, immobilization is associated with significant financial costs and the risk of enzyme activity loss during the process. To minimize these drawbacks, it is possible to use a biocatalyst in the form of mycelium-bound lipase for the synthesis of biodiesel [9][10][11]. Thus, there is no need for isolation, purification, or additional immobilization of enzymes on the selected carriers.…”

Section: Introductionmentioning

confidence: 99%

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

Kutyła,

Jaszek,

Jędrys

et al. 2023

Preprint

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In this study, biocatalysts in the form of mycelium-bound lipases of psychrotrophic and mesophilic fungi were screened for their ability to synthesize fatty acid methyl (FAME) and ethyl esters (FAEE). It has been shown that the hydrolytic activity of biocatalysts is not accompanied by their biodiesel synthetic activity. Preliminary studies showed for the first time, that the FTIR technique can be used to predict synthetic activity. The highest correlation between the intensity of the bands and synthetic activity, negative and positive, respectively, was observed for two neighboring bands at 1745 cm-1(R= −0.73) and 1699 cm-1(R= 0.68). The psychrotrophic strainPenicilliumsp. 59, isolated from the soil of the Arctic tundra (Spitsbergen), has been shown for the first time as an efficient whole-cell biocatalyst for fatty acid ethyl ester synthesis and is stable in organic solvents. Mathematical optimization of biosynthesis was also performed using RSM. A molar canola oil conversion value of 83.3% into FAEE was obtained at 24.2 °C in n-hexane using 12.5% of used canola oil and 5.3% of the fungal biocatalyst within 6 h. Dry mycelium ofPenicilliumsp. 59 is a promising new biocatalyst for large-scale biosynthesis of biodiesel, given its low-cost production, high activity at room temperature, no need to mix reactions, and reusability in a minimum of six cycles.

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Biomass-bound biocatalysts for biodiesel production: Tuning a lipolytic activity from Aspergillus niger MYA 135 by submerged fermentation using agro-industrial raw materials and waste products

Cited by 21 publications

References 50 publications

Bioconversion of Black Soldier Fly (BSF) from Organic Waste Composting into Biodiesel Assisted by Whole Cell Microbial Lipase Biocatalyst through Direct Transesterification Process

Bioconversion of Black Soldier Fly (BSF) from Organic Waste Composting into Biodiesel Assisted by Whole Cell Microbial Lipase Biocatalyst through Direct Transesterification Process

Avaliação Da Biomassa De Fungos Amazônicos Como Fonte De Lipases Para Biocatálise

Mycelium-bound lipases of psychrophilic and mesophilic fungi as biocatalysts for biodiesel synthesis

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