Biomaterial microarchitecture: a potent regulator of individual cell behavior and multicellular organization

Hogrebe, Nathaniel J.; Reinhardt, James W.; Gooch, Keith J.

doi:10.1002/jbm.a.35914

Cited by 64 publications

(57 citation statements)

References 188 publications

(441 reference statements)

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“…The phenotype of the CP5 chondrocytes on the PLA scaffolds shown by SEM is rather not typical for the native morphology in cartilage extracellular matrix. But the morphology of cells cultured under experimental conditions is in accordance with the results of previously published studies focused on proliferation of cartilage cells on fibrous scaffolds (Hogrebe et al, 2017;McCullen et al, 2012;Pilarek et al, 2014). Such phenotype differences were probably caused by two factors.…”

Section: Resultssupporting

confidence: 89%

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Pilarek

Godlewska

Kuźmińska

et al. 2017

Chemical and Process Engineering

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One of the actual challenges in tissue engineering applications is to efficiently produce as high of number of cells as it is only possible, in the shortest time. In static cultures, the production of animal cell biomass in integrated forms (i.e. aggregates, inoculated scaffolds) is limited due to inefficient diffusion of culture medium components observed in such non-mixed culture systems, especially in the case of cell-inoculated fiber-based dense 3D scaffolds, inside which the intensification of mass transfer is particularly important. The applicability of a prototyped, small-scale, continuously waveinduced agitated system for intensification of anchorage-dependent CP5 chondrocytes proliferation outside and inside three-dimensional poly(lactic acid) (PLA) scaffolds has been discussed. Fibrous PLA-based constructs have been inoculated with CP5 cells and then maintained in two independent incubation systems: (i) non-agitated conditions and (ii) culture with wave-induced agitation. Significantly higher values of the volumetric glucose consumption rate have been noted for the system with the wave-induced agitation. The advantage of the presented wave-induced agitation culture system has been confirmed by lower activity of lactate dehydrogenase (LDH) released from the cells in the samples of culture medium harvested from the agitated cultures, in contrast to rather high values of LDH activity measured for static conditions. Results of the proceeded experiments and their analysis clearly exhibited the feasibility of the culture system supported with continuously wave-induced agitation for robust proliferation of the CP5 chondrocytes on PLA-based structures. Aside from the practicability of the prototyped system, we believe that it could also be applied as a standard method offering advantages for all types of the daily routine laboratory-scale animal cell cultures utilizing various fiber-based biomaterials, with the use of only regular laboratory devices.

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Section: Resultssupporting

confidence: 89%

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Pilarek

Godlewska

Kuźmińska

et al. 2017

Chemical and Process Engineering

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“…Cell culture matrix is made of porous polymers or gels. Selection of a matrix in where cells are embedded is important in 3D culture, and the research and development in cell culture matrix have steadily progressed (Sachlos and Czernuszka 2003;Hoqrebe et al 2017).…”

Section: Introductionmentioning

confidence: 99%

The effect of three-dimensional cultured adipose tissue-derived mesenchymal stem cell–conditioned medium and the antiaging effect of cosmetic products containing the medium

Kim

Kim²,

Lee³

et al. 2019

biomed dermatol

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Background: Recently, investigators have been trying to apply the by-products as well as stem cells themselves to various fields such as pharmaceuticals, medical devices, quasi-drug, cosmetis, etc. We aimed to comfirm the antisenescence effect of 3D cultured adipose tissue-derived mesenchymal stem cell-conditioned medium (3D cultured ADMSCs-CM) and develop them as cosmetic raw materials for anti-aging purposes. Methods: We investigated the effect of 3D cultured ADMSCs-CM on collagen production and performed efficacy tests to evaluate the effect of a cream-based cosmetic product containing the medium using various methods, such as dermal density, skin moisture retention, and so on. Results: Analysis of the effect of ADMSCs-CM on skin regeneration and production of collagen showed 1.5-fold (2D cultured ADMSCs-CM) and 2.5-fold (3D cultured ADMSCs-CM) increase in expressions of procollagen and 4-fold (2D cultured ADMSCs-CM) and 5-fold (3D cultured ADMSCs-CM) increase in the expression of collagen compared with control. In addition, related gene expression was also increased. We conducted a human skin test using a cream-based product containing 3D cultured ADMSCs-CM. In skin texture assessment, skin roughness decreased by 11.94% at the application site and 3.74% at the non-application site after 3 weeks of use. Compared with before cream use, after 2 and 4 weeks of substance use, the skin elasticity analysis showed an increase in the elasticity value by 5.97% and 9.34%, respectively, and the improvement of small wrinkles was 5.01% and 6.23%, respectively. After 2 and 4 weeks of test substance use, dermal density analysis showed 6.97% and 12.53% increase, respectively. Skin moisture retention analysis showed skin moisture maintained at 543.60% and 452.38%, respectively, immediately after one-time use and after 20 min of cool breeze exposure compared with before application of the test substance. Conclusions: As raw material for cosmetic products, 3D cultured ADMSCs-CM prevented skin aging by promoting collagen production, restoring damaged skin, and increasing dermal density. Therefore, 3D cultured ADMSCs-CM can be widely applied to maintain and improve skin condition.

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“…Elucidating the mechanisms that control how cells sense and respond to biomaterials surfaces has been the focus of a large body of literature in the past three decades. [1][2][3][4][5][6][7] Results from these studies have unequivocally established that physicochemical properties such as roughness, topography, surface chemistry, energy and stiffness, direct cell fate by affecting key phenomena including adhesion, proliferation, differentiation, gene and protein expression. [8][9][10][11] Among the factors known to control cellular events, nano-topographical features play a pivotal role by exerting direct cueing on adherent cells.…”

Section: Introductionmentioning

confidence: 99%

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

Steeves¹,

Ho²,

Munisso³

et al. 2020

IJN

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Introduction: In recent years there has been ample interest in nanoscale modifications of synthetic biomaterials to understand fundamental aspects of cell-surface interactions towards improved biological outcomes. In this study, we aimed at closing in on the effects of nanotubular TiO 2 surfaces with variable nanotopography on the response on human mesenchymal stem cells (hMSCs). Although the influence of TiO 2 nanotubes on the cellular response, and in particular on hMSC activity, has already been addressed in the past, previous studies overlooked critical morphological, structural and physical aspects that go beyond the simple nanotube diameter, such as spatial statistics. Methods: To bridge this gap, we implemented an extensive characterization of nanotubular surfaces generated by anodization of titanium with a focus on spatial structural variables including eccentricity, nearest neighbour distance (NND) and Voronoi entropy, and associated them to the hMSC response. In addition, we assessed the biological potential of a twotiered honeycomb nanoarchitecture, which allowed the detection of combinatory effects that this hierarchical structure has on stem cells with respect to conventional nanotubular designs. We have combined experimental techniques, ranging from Scanning Electron (SEM) and Atomic Force (AFM) microscopy to Raman spectroscopy, with computational simulations to characterize and model nanotubular surfaces. We evaluated the cell response at 6 hrs, 1 and 2 days by fluorescence microscopy, as well as bone mineral deposition by Raman spectroscopy, demonstrating substrate-induced differential biological cueing at both the short-and long-term. Results: Our work demonstrates that the nanotube diameter is not sufficient to comprehensively characterize nanotubular surfaces and equally important parameters, such as eccentricity and wall thickness, ought to be included since they all contribute to the overall spatial disorder which, in turn, dictates the overall bioactive potential. We have also demonstrated that nanotubular surfaces affect the quality of bone mineral deposited by differentiated stem cells. Lastly, we closed in on the integrated effects exerted by the superimposition of two dissimilar nanotubular arrays in the honeycomb architecture. Discussion: This work delineates a novel approach for the characterization of TiO 2 nanotubes which supports the incorporation of critical spatial structural aspects that have been overlooked in previous research. This is a crucial aspect to interpret cellular behaviour on nanotubular substrates. Consequently, we anticipate that this strategy will contribute to the unification of studies focused on the use of such powerful nanostructured surfaces not only for biomedical applications but also in other technology fields, such as catalysis.

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Biomaterial microarchitecture: a potent regulator of individual cell behavior and multicellular organization

Cited by 64 publications

References 188 publications

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

Enhanced Chondrocyte Proliferation in a Prototyped Culture System with Wave-Induced Agitation

The effect of three-dimensional cultured adipose tissue-derived mesenchymal stem cell–conditioned medium and the antiaging effect of cosmetic products containing the medium

<p>The Implication of Spatial Statistics in Human Mesenchymal Stem Cell Response to Nanotubular Architectures</p>

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