Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study

Fernandes, Kelly Rossetti; Zhang, Yang; Magri, Angela Maria Paiva; Rennó, Ana Cláudia Muniz; Beucken, Jeroen J.J.P. van den

doi:10.1021/acsbiomaterials.7b00679

Cited by 38 publications

(36 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gu et al [ 64 , 65 ] explored whether introducing a certain amount of β-TCP into calcium phosphate cement could promote M polarization to the anti-inflammatory phenotype (M2) in long-term culture. In contrast, Tai et al [ 66 ], Chen et al [ 67 ], and Fernandes et al [ 68 ] found that β-TCP, its extract, and its use as a coating on a Ti surface were strong enough to induce M1 polarization of M s. Chen et al [ 69 ] also identified that β-TCP maintained a high proportion of iNOS + M1-polarized M s both in vitro and in vivo. In our study, TCP was wrapped in PLLA to form a porous structure.…”

Section: Discussionmentioning

confidence: 99%

Sustained zinc release in cooperation with CaP scaffold promoted bone regeneration via directing stem cell fate and triggering a pro-healing immune stimuli

Huang

Zhu

et al. 2021

J Nanobiotechnol

View full text Add to dashboard Cite

Metal ions have been identified as important bone metabolism regulators and widely used in the field of bone tissue engineering, however their exact role during bone regeneration remains unclear. Herein, the aim of study was to comprehensively explore the interactions between osteoinductive and osteo-immunomodulatory properties of these metal ions. In particular, the osteoinductive role of zinc ions (Zn2+), as well as its interactions with local immune microenvironment during bone healing process, was investigated in this study using a sustained Zn2+ delivery system incorporating Zn2+ into β-tricalcium phosphate/poly(L-lactic acid) (TCP/PLLA) scaffolds. The presence of Zn2+ largely enhanced osteogenic differentiation of periosteum-derived progenitor cells (PDPCs), which was coincident with increased transition from M1 to M2 macrophages (M$$\varphi $$ φ s). We further confirmed that induction of M2 polarization by Zn2+ was realized via PI3K/Akt/mTOR pathway, whereas marker molecules on this pathway were strictly regulated by the addition of Zn2+. Synergically, this favorable immunomodulatory effect of Zn2+ further improved the osteogenic differentiation of PDPCs induced by Zn2+ in vitro. Consistently, the spontaneous osteogenesis and pro-healing osteoimmunomodulation of the scaffolds were thoroughly identified in vivo using a rat air pouch model and a calvarial critical-size defect model. Taken together, Zn2+-releasing bioactive ceramics could be ideal scaffolds in bone tissue engineering due to their reciprocal interactions between osteoinductive and immunomodulatory characteristics. Clarification of this synergic role of Zn2+ during osteogenesis could pave the way to develop more sophisticated metal-ion based orthopedic therapeutic strategies.

show abstract

Section: Discussionmentioning

confidence: 99%

Sustained zinc release in cooperation with CaP scaffold promoted bone regeneration via directing stem cell fate and triggering a pro-healing immune stimuli

Huang

Zhu

et al. 2021

J Nanobiotechnol

View full text Add to dashboard Cite

show abstract

“…More comprehensive studies are required to pinpoint the surface properties most relevant for specific biological responses, as well as to eliminate bias when comparing results from different experimental concepts (cellular models, interspecies differences, and readout parameters). For this reason, we employed three in vitro models of the human immune system, ranging in degree of biological complexity from simple to complex: We chose a model based on THP-1-derived macrophages because this is widely regarded as a valid model to investigate biomaterials, particularly due to its demonstrated ability to relate to the host response in vivo [18][19][20][21]. Due to its reduced complex biology, this model also affords several advantages.…”

Section: Introductionmentioning

confidence: 99%

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro

Segan

Jakobi

Khokhani

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

It has been widely shown that biomaterial surface topography can modulate host immune response, but a fundamental understanding of how different topographies contribute to pro-inflammatory or anti-inflammatory responses is still lacking. To investigate the impact of surface topography on immune response, we undertook a systematic approach by analyzing immune response to eight grades of medical grade polyurethane of increasing surface roughness in three in vitro models of the human immune system. Polyurethane specimens were produced with defined roughness values by injection molding according to the VDI 3400 industrial standard. Specimens ranged from 0.1 μm to 18 μm in average roughness (Ra), which was confirmed by confocal scanning microscopy. Immunological responses were assessed with THP-1-derived macrophages, human peripheral blood mononuclear cells (PBMCs), and whole blood following culture on polyurethane specimens. As shown by the release of pro-inflammatory and anti-inflammatory cytokines in all three models, a mild immune response to polyurethane was observed, however, this was not associated with the degree of surface roughness. Likewise, the cell morphology (cell spreading, circularity, and elongation) in THP-1-derived macrophages and the expression of CD molecules in the PBMC model on T cells (HLA-DR and CD16), NK cells (HLA-DR), and monocytes (HLA-DR, CD16, CD86, and CD163) showed no influence of surface roughness. In summary, this study shows that modifying surface roughness in the micrometer range on polyurethane has no impact on the pro-inflammatory immune response. Therefore, we propose that such modifications do not affect the immunocompatibility of polyurethane, thereby supporting the notion of polyurethane as a biocompatible material.

show abstract

“…The surface-adsorbed proteins participate in processes including clot formation, tissue granulation and cell recruitment, in a coordinated manner, with direct cross-talk between the osteogenic and immune cells [14].The interplay between immunology systems and bone forming cells is critical for osseointegration and to understand microenvironmental and cellular cues [15]. Specifically the role of macrophages has recently been recognized as critical for bone homeostasis, directly affecting the cross-talk between osteoblasts and osteoclasts [16,17].…”

Section: Introductionmentioning

confidence: 99%

Complement proteins regulating macrophage polarisation on biomaterials

Araújo‐Gomes

Romero‐Gavilán

Zhang

et al. 2019

Colloids and Surfaces B: Biointerfaces

View full text Add to dashboard Cite

One of the events occurring when a biomaterial is implanted in an host is the protein deposition onto its surface, which might regulate cell responses. When a biomaterial displays a compromised biocompatibility, distinct complement pathways can be activated to produce a foreign body reaction. In this article, we have designed different types of biomaterial surfaces to study the inflammation process. Here, we used different concentrations of (3-glycidoxypropyl)-trimethoxysilane (GPTMS), an organicallymodified alkoxysilane as a precursor for the synthesis of various types of sol-gel materials functionalizing coatings for titanium implants to regulate biological responses. Our results showed that increasing GPTMS concentration slightly enhanced the osteogenic potential of MC3T3-E1 cells in vitro, which seems linked to upregulation of TGF-β expression. Moreover, greater GPTMS surface concentrations induced greater secretion of TNF-α and IL-10 on RAW 264.7 macrophages. When implanted into rabbit tibia, osseointegration decreased with higher GPTMS concentrations. Interestingly, higher deposition of complement-related proteins C-reactive protein (CRP) and ficolin-2 (FCN2), two main activators of distinct complement pathways, was observed. Taking all together, inflammatory potential increase seems to be GPTMS concentrationdependent. Our results show that a greater adsorption of complement proteins can condition macrophage polarization.

show abstract

Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study

Cited by 38 publications

References 41 publications

Sustained zinc release in cooperation with CaP scaffold promoted bone regeneration via directing stem cell fate and triggering a pro-healing immune stimuli

Sustained zinc release in cooperation with CaP scaffold promoted bone regeneration via directing stem cell fate and triggering a pro-healing immune stimuli

Systematic Investigation of Polyurethane Biomaterial Surface Roughness on Human Immune Responses in vitro

Complement proteins regulating macrophage polarisation on biomaterials

Contact Info

Product

Resources

About