Biomaterial topography alters healing in vivo and monocyte/macrophage activation in vitro

Abstract

The effect of biomaterial topography on healing in vivo and monocyte/macrophage stimulation in vitro was assessed. A series of expanded polytetrafluoroethylene (ePTFE) materials were characterized by increasing average intranodal distance of 1.2 µm (1.2-ePTFE), 3.0 µm (3.0-ePTFE) and 4.4 µm (4.4-ePTFE), but presented consistent surface chemistry with nonporous PTFE (np-PTFE). Subcutaneous implantation of 4.4-ePTFE into mice resulted in a statistically thinner capsule that appeared less organized and less dense than the np-PTFE response. In vitro, isolated monocytes/macrophages cultured on np-PTFE produced low levels of interleukin 1-beta (IL-1β), 1.2-ePTFE and 3.0-ePTFE stimulated intermediate levels, and 4.4-ePTFE stimulated a 15-fold increase over np-PTFE. Analysis of cDNA microarrays demonstrated that additional proinflammatory cytokines and chemokines, including IL-1β, interleukin 6, tumor necrosis factor alpha, monocyte chemotactic protein 1 and macrophage inflammatory protein 1-beta, were expressed at higher levels by monocytes/macrophages cultured on 4.4-ePTFE at four and twenty-four hours. Expression ratios for several genes were quantified by RT-PCR and were consistent with those from the cDNA array results. These results demonstrate the effect of biomaterial topography on early proinflammatory cytokine production and gene transcription by monocytes/macrophages in vitro as well as decreased fibrous capsule thickness in vivo.

Biochemical and Biophysical Cues in Matrix Design for Chronic and Diabetic Wound Treatment

“…178 This provides possible explanation for the improved healing and was further proved in vitro, where the monocyte/macrophage activation was significantly higher on porous surfaces. 179 Negative pressure. Topical negative pressure (TNP) therapy is a long established practice as an adjunctive therapy for chronic wounds since 1980s.…”

Effects of Titanium Surfaces on the Developmental Profile of Monocytes/Macrophages

“…Such findings cannot be directly compared with data from the literature because differences in experimental models and biomaterials may have produced the discrepancies in the results (13,16). However, it is important to point out that primary cells obtained from peripheral blood are closer to clinical conditions than animal cells or cell lines (25).…”

“…In other words, the differential wound healing responses elicited by the different biomaterials might be due to the different macrophage functional profiles induced by the materials' surfaces (12). Although the magnitude of the inflammatory response for an optimal bone response is unknown (6), compelling data have increased interest in understanding macrophage activation in the context of biomaterials (12)(13)(14)(15)(16).…”

“…Few studies compare the effects of different titanium surfaces on macrophage activation or consider the role of material surface roughness on protein adsorption (9,15,16). Accumulating evidence suggests that the interactions between macrophages and adsorbed proteins on the material surface provide intracellular signals that are essential for regulating cell function (6,12,14).…”

Functional Activity of Macrophage Cells in the Assessment of Reaction to Biodegradable Implants

“…Ма-крофаги с фенотипом М2 экспрессируют скавенджер-рецепторы, продуцируют хемо-кины и факторы роста, которые индуцируют процессы ангиогенеза и тканевой репарации (Brown et al, 2012;Garg et al, 2013;Sussman et al, 2014;Wolf et al, 2014;Moore et al, 2015). McKeon-Fischer and Freeman, 2011;McKeonFischer et al, 2015;Vakilian et al, 2015), что может определять вариабельность процессов адгезии моноцитов, их активации, а впослед-ствии и вариабельность функциональных фе-нотипов макрофагов (Saino et al, 2011;Bota et al, 2010). Это определяет особую актуаль-ность изучения влияния микротопографии биоматериалов на процессы дифференциров-ки моноцитов-макрофагов.…”