Biomaterials and Stem Cell Therapies for Injuries Associated to Skeletal Muscular Tissues

Pereira, Tiago; Gärtner, Andrea; Amorim, Irina; Armada-da-Silva, Paulo; Gomes, Rui; Pereira, Catarina; França, Miguel Lacueva; Morais, Diana M.; Rodrigues, Marco Aurélio; Lopes, M. C. A.; Santos, J. D.; Luís, Ana Lúcia; Maurício, Ana Colette

doi:10.5772/53335

Cited by 14 publications

(41 citation statements)

References 105 publications

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“…However, less acute ankle joint angles observed in PVACNTs-MSCs group, compared with PVA-PPy and PVA-CNTs groups might suggest improved ankle muscles function during the push off phase of the rat's gait cycle ( Figure 4 and Table 3). It is well known and demonstrated by the scientific bibliography that neuromuscular pathologies are related to important clinical signs or motor deficits that should be observed, qualified, and quantified, only possible with a precise kinematic analysis [65,66] . In the field of peripheral nerve research using the rat sciatic nerve model, an improved walking analysis might include several methods combination like joint kinematics, ground reaction forces and electromyographical data of muscle activity.…”

Section: Discussionmentioning

confidence: 99%

“…One evident advantage is that using a treadmill the operator reduces step-by-step variability in joint kinematics [59] which has the disadvantage of being expensive equipment. Also the possibility of combining kinematic analysis with other data, such as ground reaction forces, should be consider for a more accurate evaluation of nerve regeneration [59,64,65] . Our research group has recently analyzed hip, knee and ankle joint kinematics during recovery of rat sciatic nerve axonotmesis injury, using reflective markers attached to the rat hind-limb to track the motion with infra-red capture cameras, to better assess the rat sciatic nerve model hind-limb joint kinematics during walking.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Ribeiro¹,

Pereira²,

Caseiro³

et al. 2015

WJSC

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“…Although the general intrinsic regenerative response of the skeletal muscle tissue to an injury event (approached in detail earlier in this chapter) follows a mostly constant sequence of events, the source and the magnitude of the induced injury lead to distinct native healing efficiencies and consequently distinct responses to implemented therapies [27,104]. It results into an additional challenge when trying to understand the true magnitude of effects, especially in attempts of comparative analysis on the available literature.…”

Section: Cell Sourcementioning

confidence: 99%

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

Caseiro¹,

Pereira²,

Bártolo³

et al. 2015

Progress in Stem Cell Transplantation

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Skeletal muscle injuries are quite frequent in traumatic scenarios, such as war injuries or road-or work-related accidents. The skeletal muscle has good regenerative ability, but the extent or recurrence of muscle injury might impair complete structural and functional recovery. Severe tissue loss overwhelms skeletal muscle´s intrinsic regenerative capabilities and culminates in the development of noncontractile fibrous tissue scar. Conservative RICE -based and surgical treatments show limited efficacy in terms of improving these severe cases outcomes, pressing the need for new approaches on skeletal muscle's therapy. Since the first suggestions of the potential of mesenchymal stem cells for regenerative medicine and tissue engineering, many applications have been explored for a variety of tissues and diseases, including the skeletal muscle, which is the focus of this literature review.

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“…They can be used as a physical barrier to protect the cells from hostile extrinsic factors or as a matrix to cell adhesion, growth and differentiation to further improve the secretion of therapeutic proteins by the cells. 30 When considering the use of these neural scaffolds in the clinic there are some requirements that they have to fulfil such as being easy to fabricate and sterilize, simple to implant in the body by microsurgical techniques, biocompatible and biodegradable.…”

Section: Tissue Engineered Nerve Graftsmentioning

confidence: 99%

“…28 Its biodegradability, biocompatibility, non-antigenicity, notable affinity to proteins, promotion of cell adhesion and non-toxicity has made it possible to use chitosan in a number of biomedical applications, including wound dressings, drug delivery systems and space-filing implants. 30,48 It has also seen its application in the tissue engineering field increase largely, serving as a candidate biomaterial for the development of neural scaffolds. 1 Being fragile in the dried form, chitosan can be chemically crosslinked or jointly used with other materials before scaffold fabrication.…”

Section: Chitosanmentioning

confidence: 99%

Regeneration of the peripheral nerve — Development and evaluation of guide tubes of biodegradable polymer

Gomes

Santos

Maurício

2017

2017 IEEE 5th Portuguese Meeting on Bioengineering (ENBENG)

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ii © Joana Gomes, 2014 iii iv ResumoDanos nas fibras nervosas periféricas resultam em perda axonal e desmielinização, seguidos de regeneração e remielinização sob condições ideais com a possibilidade de alguma recuperação funcional. Estudos recentes indicam que a diferença na regeneração axonal entre o sistema nervoso periférico (PNS) e o sistema nervoso central (CNS) resulta da presença de fatores permissivos presentes no PNS e fatores inibitórios ativos presentes no CNS.Pacientes com lesões no nervo periférico estão muitas vezes no auge da sua atividade laboral, o que faz da regeneração do nervo periférico um foco principal de pesquisa. Assim, o desafio experimental consiste em acelerar a regeneração axonal para promover a reinervação e melhorar a recuperação funcional após uma lesão no nervo periférico. Algumas das abordagens experimentais para melhorar a recuperação funcional incluem a aplicação focal de fatores neurotróficos, o bloqueio de moléculas inibidoras da regeneração axonal e o transplante de células.Depois de ocorrer uma lesão quer de axonotmese quer de neurotmese, os axónios periféricos têm a capacidade de se regenerar e de se ligar novamente aos seus alvos, sendo que no primeiro caso não há necessidade de reconstrução cirúrgica uma vez que o endoneuro, perineuro e epineuro são mantidos. No entanto, o estado funcional resultante pode não ser o melhor uma vez que a regeneração é demorada e conduz à atrofia neurogénica dos músculos regionais. Na prática clínica, a reparação cirúrgica ideal em situações de neurotmese (seccionamento total do nervo) considera-se ser a sutura topo-a-topo, de modo a aproximar o topo distal do topo proximal, sem haver tensão no local da sutura. Alternativamente, nervos autólogos são o gold standard deste tipo de procedimentos sempre que ocorre perda de substância e não seja possível executar uma sutura topo-a-topo sem tensão. No entanto, o uso de autoenxertos está limitado pelos inconvenientes inerentes uma vez que requer uma segunda cirurgia, com sacrifício de um nervo funcional, o que resulta em perda sensorial e morbidade do local de doação, entre outros.Nas últimas décadas, os diferentes tipos de enxertos artificiais ou biológicos foram desenvolvidos e investigados para que pudesse ser estabelecida uma relação entre eles e os enxertos de nervos autólogos em termos dos resultados da regeneração do nervo e da v recuperação funcional. Estes tubos-guia são projetados para preencher a lacuna existente num nervo seccionado quando existe um gap, para limitar a fibrose e orientar as fibras em regeneração na direção do topo distal. O enxerto de nervo artificial é um canal tubular feito de materiais sintéticos ou biodegradáveis e que pode orientar a regeneração do axónio sem inibir o crescimento e maturação. O canal tubular pode ser implantado vazio ou preenchido com fatores de crescimento, células ou fibras. Devido à sua capacidade de renovação, multiplicação e diferenciação, as células estaminais mesenquimatosas (MSCs) têm sido usadas para suplementar os tubos-guia, sendo ...

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Biomaterials and Stem Cell Therapies for Injuries Associated to Skeletal Muscular Tissues

Cited by 14 publications

References 105 publications

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Evaluation of biodegradable electric conductive tube-guides and mesenchymal stem cells

Trends in Mesenchymal Stem Cells' Applications for Skeletal Muscle Repair and Regeneration

Regeneration of the peripheral nerve — Development and evaluation of guide tubes of biodegradable polymer

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