Biomaterials from Decellularized Tissues

Londoño, Ricardo; Badylak, Stephen F.

doi:10.1002/9781119126218.ch12

Cited by 6 publications

(4 citation statements)

References 206 publications

(224 reference statements)

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“…Among the many processes available for creating 3D environments, the most successful ones are summarized in the following section. Special mention goes to 3D bioprinting [212][213][214][215] and decellularized scaffolds [216][217][218], which offer promising potential in terms of signalling modulation and biomimetism. In terms of macrodimensionality, 3D additive manufacturing techniques represent some of the most promising and flexible methods to process artificial skin tissue for wounds because of their versatility, reproducibility and low-cost [219,220].…”

Section: Physical Signalingmentioning

confidence: 99%

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Castaño

Pérez‐Amodio

Nieva

et al. 2018

Advanced Drug Delivery Reviews

168

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Skin wound healing aims to repair and restore tissue through a multistage process that involves different cells and signalling molecules that regulate the cellular response and the dynamic remodelling of the extracellular matrix. Nowadays, several therapies that combine biomolecule signals (growth factors and cytokines) and cells are being proposed. However, a lack of reliable evidence of their efficacy, together with associated issues such as high costs, a lack of standardization, no scalable processes, and storage and regulatory issues, are hampering their application. In situ tissue regeneration appears to be a feasible strategy that uses the body's own capacity for regeneration by mobilizing host endogenous stem cells or tissue-specific progenitor cells to the wound site to promote repair and regeneration. The aim is to engineer instructive systems to regulate the spatio-temporal delivery of proper signalling based on the biological mechanisms of the different events that occur in the host microenvironment. This review describes the current state of the different signal cues used in wound healing and skin regeneration, and their combination with biomaterial supports to create instructive microenvironments for wound healing.

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Section: Physical Signalingmentioning

confidence: 99%

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Castaño

Pérez‐Amodio

Nieva

et al. 2018

Advanced Drug Delivery Reviews

168

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“…The extracellular matrix is a bioactive scaffold that contains many natural polymers that have the capacity to promote various types of tissue-specific cues, giving biological cues to the microenvironment surrounding the scaffold and ultimately promoting tissue regeneration [ 64 ]. Growth factors (GFs) are blood-derived peptide factors that direct inflammatory cells to migrate onto the wound, attract fibroblasts, and stimulate cell proliferation [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies

et al. 2023

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Background: Hydrogel is considered a promising scaffold biomaterial for gingival regeneration. In vitro experiments were carried out to test new potential biomaterials for future clinical practice. The systematic review of such in vitro studies could synthesize evidence of the characteristics of the developing biomaterials. This systematic review aimed to identify and synthesize in vitro studies that assessed the hydrogel scaffold for gingival regeneration. Methods: Data on experimental studies on the physical and biological properties of hydrogel were synthesized. A systematic review of the PubMed, Embase, ScienceDirect, and Scopus databases was conducted according to the Preferred Reporting System for Systematic Reviews and Meta-Analyses (PRISMA) 2020 statement guidelines. In total, 12 original articles on the physical and biological properties of hydrogels for gingival regeneration, published in the last 10 years, were identified. Results: One study only performed physical property analyses, two studies only performed biological property analyses, and nine studies performed both physical and biological property analyses. The incorporation of various natural polymers such as collagen, chitosan, and hyaluronic acids improved the biomaterial characteristics. The use of synthetic polymers faced some drawbacks in their physical and biological properties. Peptides, such as growth factors and arginine–glycine–aspartic acid (RGD), can be used to enhance cell adhesion and migration. Based on the available primary studies, all studies successfully present the potential of hydrogel characteristics in vitro and highlight the essential biomaterial properties for future periodontal regenerative treatment.

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“…Ideally, artificial surface reaction through current non-biological devices could be avoided and, as a result, TEVG would be implanted in tissue with fewer inflammatory alterations. Constructive remodeling [ 46 ] and further improved patency rates could be obtained by this strategy. In order to acquire readily available and therefore transplantable scaffolds for recellularization, discarded BCAs were decellularized using a combination of decellularization techniques for gentle yet effective cell removal.…”

Section: Discussionmentioning

confidence: 99%

“…We therefore assumed co-implantation could help both cell types to maintain their respective cell function and enable remodeling as well as anastomosis healing upon implantation in vivo and exposure to patient-specific serum. Additionally, MSC have shown to be both, immune evasive and immunosuppressive and can therefore create a low inflammatory environment [ 60 ], suitable for constructive remodeling [ 46 ]. To explore our hypothesis we used both rat bone-marrow MSC (rMSC) due to easy obtainability and reported low immunogenicity [ 61 ] as well as human umbilical cord MSC (hMSC).…”

Section: Discussionmentioning

confidence: 99%

In vitro recellularization of decellularized bovine carotid arteries using human endothelial colony forming cells

et al. 2021

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Background Many patients suffering from peripheral arterial disease (PAD) are dependent on bypass surgery. However, in some patients no suitable replacements (i.e. autologous or prosthetic bypass grafts) are available. Advances have been made to develop autologous tissue engineered vascular grafts (TEVG) using endothelial colony forming cells (ECFC) obtained by peripheral blood draw in large animal trials. Clinical translation of this technique, however, still requires additional data for usability of isolated ECFC from high cardiovascular risk patients. Bovine carotid arteries (BCA) were decellularized using a combined SDS (sodium dodecyl sulfate) -free mechanical-osmotic-enzymatic-detergent approach to show the feasibility of xenogenous vessel decellularization. Decellularized BCA chips were seeded with human ECFC, isolated from a high cardiovascular risk patient group, suffering from diabetes, hypertension and/or chronic renal failure. ECFC were cultured alone or in coculture with rat or human mesenchymal stromal cells (rMSC/hMSC). Decellularized BCA chips were evaluated for biochemical, histological and mechanical properties. Successful isolation of ECFC and recellularization capabilities were analyzed by histology. Results Decellularized BCA showed retained extracellular matrix (ECM) composition and mechanical properties upon cell removal. Isolation of ECFC from the intended target group was successfully performed (80% isolation efficiency). Isolated cells showed a typical ECFC-phenotype. Upon recellularization, co-seeding of patient-isolated ECFC with rMSC/hMSC and further incubation was successful for 14 (n = 9) and 23 (n = 5) days. Reendothelialization (rMSC) and partial reendothelialization (hMSC) was achieved. Seeded cells were CD31 and vWF positive, however, human cells were detectable for up to 14 days in xenogenic cell-culture only. Seeding of ECFC without rMSC was not successful. Conclusion Using our refined decellularization process we generated easily obtainable TEVG with retained ECM- and mechanical quality, serving as a platform to develop small-diameter (< 6 mm) TEVG. ECFC isolation from the cardiovascular risk target group is possible and sufficient. Survival of diabetic ECFC appears to be highly dependent on perivascular support by rMSC/hMSC under static conditions. ECFC survival was limited to 14 days post seeding.

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Biomaterials from Decellularized Tissues

Cited by 6 publications

References 206 publications

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Instructive microenvironments in skin wound healing: Biomaterials as signal releasing platforms

Hydrogel-Based Biomaterial as a Scaffold for Gingival Regeneration: A Systematic Review of In Vitro Studies

In vitro recellularization of decellularized bovine carotid arteries using human endothelial colony forming cells

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