Biomechanical Analysis of a Chondrocyte-Based Repair Model of Articular Cartilage

Peretti, Giuseppe M.; Bonassar, Lawrence J.; Caruso, Enzo M.; Randolph, Mark A.; Trahan, Carol; Zaleske, David J.

doi:10.1089/ten.1999.5.317

Cited by 64 publications

(62 citation statements)

References 29 publications

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“…Previous studies of our group demonstrated the feasibility and the efficiency of bonding cartilaginous [25,26,28,32] and meniscal [27,29] tissue with cellular fibrin glue. According to this experience, we have designed the present study in order to evaluate the potential of chondrocytes-embedded fibrin glue for bonding meniscal slices in a nude mouse model.…”

Section: Discussionmentioning

confidence: 88%

Healing of meniscal tissue by cellular fibrin glue: an in vivo study

Scotti

Pozzi

Mangiavini

et al. 2009

Knee Surg Sports Traumatol Arthrosc

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Menisci represent fundamental structures for the maintenance of knee homeostasis, playing a key role in knee biomechanics. However, their intrinsic regenerative potential is poor. As a consequence, when a lesion occurs and the meniscus is partially removed by surgery, knee mechanics is subject to dramatic changes. These have been demonstrated to lead often to the development of early osteoarthritis. Therefore, menisci should be repaired whenever possible. In the last decades, tissue engineering approaches have been advocated to improve the reparative processes of joint tissues. In this study, the bonding capacity of an articular chondrocytes-fibrin glue hydrogel was tested as a biologic glue to improve the bonding between two swine meniscal slices in a nude mouse model. The composites were wrapped with acellular fibrin glue and implanted in subcutaneous pouches of nude mice for 4 weeks. Upon retrieval, a firm gross bonding was observed in the experimental samples while none of the control samples, prepared with acellular fibrin glue at the interface, presented any sign of bonding. This was consistent with the histological and scanning electron microscope findings. In particular, a fibrocartilaginous tissue was found at the interface between the meniscal slices, partially penetrating the native meniscus tissue. In order to overcome the lack of regenerative properties of the meniscus, the rationale of using cellular fibrin glue is that fibrin provides immediate stability while carrying cells in the site of lesion. Moreover, fibrin gel is recognized as an optimal scaffold for cell embedding and for promoting fibrocartilaginous differentiation of the cells which synthesize matrix having healing property. These results demonstrated the potential of this model for improving the meniscal bonding. However, further orthotopic studies in a large animal model are needed to evaluate its potential for clinical application.

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Section: Discussionmentioning

confidence: 88%

Healing of meniscal tissue by cellular fibrin glue: an in vivo study

Scotti

Pozzi

Mangiavini

et al. 2009

Knee Surg Sports Traumatol Arthrosc

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show abstract

“…According to our previous experience with chondrocytes and fibrin glue [26][27][28][29][30], we have designed the present study in order to investigate the potential of a tissue engineered implant, made of chondrocytes expanded in vitro, fibrin glue, and a calcium-phosphate scaffold, for osteochondral repair. We have analyzed the macroscopic appearance of this composite and the resulting stiffness of the constructs after in vitro culture.…”

Section: Discussionmentioning

confidence: 99%

A tissue engineered osteochondral plug: an in vitro morphological evaluation

Scotti

Buragas

Mangiavini

et al. 2007

Knee Surg Sports Traumatol Arthr

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Articular cartilage lesions have a poor intrinsic healing potential. The repair tissue is often fibrous, having insufficient biomechanical properties, which could frequently lead to the development of early osteoarthritis. In the last decade, tissue engineering approaches addressed this topic in order to restore joint function with a differentiated and functional tissue. Many biomaterials and techniques have been proposed and some of them applied in clinical practice, even though several concerns have been raised on the quality of the engineered tissue and on its integration in the host joint. In this study, we focused on engineering in vitro a biphasic composite made of cellular fibrin glue and a calcium-phosphate scaffold. Biphasic composites are the latest products of tissue engineering applied to articular cartilage and they seem to allow a more efficient integration of the engineered tissue with the host. However, a firm in vitro bonding between the two components of the composite is a necessary condition to validate this model. Our study demonstrated a gross and microscopic integration of the two components and a cartilage-like quality of the newly formed matrix. Moreover, we noticed an improvement of this integration and GAGs production during the in vitro culture.

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“…This study evaluated the morphologic characteristics of an in vitro tissue-engineered osteochondral composite designed to treat osteochondral lesions. Following our previous experience with chondrocyte-fibrin glue composites [17][18][19][20], we designed the present study to investigate the potential of a tissue-engineered implant, made of cultured chondrocytes, fibrin glue, and a calciumphosphate scaffold. The gross mechanical integrity of the fresh specimens, evaluated by probing with forceps, was consistent with cartilage formation.…”

Section: Discussionmentioning

confidence: 99%

An in vitro tissue-engineered model for osteochondral repair

et al. 2006

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One of the main topics of regenerative medicine and tissue engineering is to address the problem of lesions involving articular cartilage. In fact, these lesions do not heal spontaneously and often lead to osteoarthritis, which causes chronic pain and worsens quality of life. Moreover, the only available treatment for osteoarthritis is symptomatic therapy and prosthetic replacement, with far from satisfactory results. A more conservative approach that restores the articular surface and function with a biologic tissue is desirable. Several strategies for regenerating articular cartilage have been proposed and applied in clinical practice but a gold standard has not yet been identified. Biphasic composites are the latest products of tissue engineering applied to articular cartilage and they seem to permit a more efficient integration of the engineered neo-tissue with the host. We present an in vitro tissue engineered model for osteochondral repair based on a composite of chondrocytes-fibrin glue gel and a calciumphosphate scaffold. This composite showed a gross integration of the two components and a cartilage-like quality of the newly formed matrix. Further studies are planned to quantify the adherence between the scaffold and the cellular fibrin glue.

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Biomechanical Analysis of a Chondrocyte-Based Repair Model of Articular Cartilage

Cited by 64 publications

References 29 publications

Healing of meniscal tissue by cellular fibrin glue: an in vivo study

Healing of meniscal tissue by cellular fibrin glue: an in vivo study

A tissue engineered osteochondral plug: an in vitro morphological evaluation

An in vitro tissue-engineered model for osteochondral repair

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