Biomechanical and biophysical environment of bone from the macroscopic to the pericellular and molecular level

Ren, Li; Yang, Pengfei; Wang, Zhe; Zhang, Jian; Ding, Chong; Shang, Peng

doi:10.1016/j.jmbbm.2015.04.021

Cited by 48 publications

(30 citation statements)

References 160 publications

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“…A low magnitude, dynamic HP stimulus, consistent with that found within bone, is sufficient to drive MSC osteogenesis in vitro. MSCs were stimulated with both static and dynamic (cyclic, 1 Hz) pressures of 100 kPa magnitude, which has been predicted to occur in vivo (39). We found that pressure, independent of fluid shear, elicits a pro-osteogenic response in MSCs depicted by up-regulation of bone markers Cox2, Runx2, and Opn.…”

Section: Discussionmentioning

confidence: 77%

“…Fluid shear stress is minimal as no valves are open to allow fluid to flow (16). The pressure bioreactor was validated for a pressure of 100 kPa at 1 Hz frequency, which is a magnitude predicted to occur within bone and bone marrow, whereas the chosen frequency is associated with human locomotion (39,40). In addition, a constant 100 kPa HP was employed, as it is a commonly used mechanical regime for in vitro pressure mechanobiology studies (8,29,41).…”

Section: Hp and Cyclic Hp Mechanical Stimulationmentioning

confidence: 99%

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Pressure‐induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling

Stavenschi

Hoey

2018

FASEB j.

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Macroscale loading of bone generates a complex local mechanical microenvironment that drives osteogenesis and bone mechanoadaptation. One such mechanical stimulus generated is hydrostatic pressure (HP); however, the effect of HP on mesenchymal stem cells (MSCs) and the mechanotransduction mechanisms utilized by these cells to sense this stimulus are yet to be fully elucidated. In this study, we demonstrate that cyclic HP is a potent mediator of cytoskeletal reorganization and increases in osteogenic responses in MSCs. In particular, we demonstrate that the intermediate filament (IF) network undergoes breakdown and reorganization with centripetal translocation of IF bundles toward the perinuclear region. Furthermore, we show for the first time that this IF remodeling is required for loading‐induced MSC osteogenesis, revealing a novel mechanism of MSC mechanotransduction. In addition, we demonstrate that chemical disruption of IFs with withaferin A induces a similar mechanism of IF breakdown and remodeling as well as a subsequent increase in osteogenic gene expression in MSCs, exhibiting a potential mechanotherapeutic effect to enhance MSC osteogenesis. This study therefore highlights a novel mechanotransduction mechanism of pressure‐induced MSC osteogenesis involving the understudied cytoskeletal structure, the IF, and demonstrates a potential new therapy to enhance bone formation in bone‐loss diseases such as osteoporosis.—Stavenschi, E., Hoey, D. A. Pressure‐induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling. FASEB J. 33, 4178–4187 (2019). http://www.fasebj.org

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Section: Discussionmentioning

confidence: 77%

Section: Hp and Cyclic Hp Mechanical Stimulationmentioning

confidence: 99%

Pressure‐induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling

Stavenschi

Hoey

2018

FASEB j.

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“…In TE studies, one of bone's characteristics resembles vascular tissue in as much as it allows the perfusions of nutrients and oxygen. In vivo, loadings that are applied to bone cause fluid to flow through the bone, while shear stress influence osteoblasts and osteocytes to differentiate and mineralize [24]. Therefore in bioreactors designed for bone formation, shear stress and perfusion are constantly highlighted.…”

Section: Bioreactors For Bone Tissue Expansionmentioning

confidence: 99%

Bioreactors for tissue engineering: An update

Zhao

Griffin

Cai

et al. 2016

Biochemical Engineering Journal

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One of the greatest puzzles in wound healing is how to substitute or replace the defect caused by loss and damaged tissue or organs. In regenerative medicine, Tissue Engineering has been proposed to supply this demand by generating tissues in vitro. Bioreactors are the key to translate these cells and tissue-based constructs into large-scale biological products that are clinically effective, safe and financially pliable. In this review, we summarise the different upto-date bioreactor designs being used for different cell types and special design scaffolds, and highlight advantages of different bioreactors, current challenges and the future trends. It is our belief that with efforts combined from multiple disciplinary participants, a novel bioreactor system that is capable of fast, large scale tissue culture would come about in near future.

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“…Bone is complex not only in its hierarchical structure with anisotropic mechanical properties but also in its electromechanical properties. Various methods have been developed for comprehensive understanding of the electromechanical properties of bone (Atsushi, 2015;Hastings, 1988;Isaacson and Bloebaum, 2010;Qin et al, 2005;Qin and Ye, 2004;Ren et al, 2015;Rosa et al, 2015). It was found that when a bone is being loaded, its piezo-voltage decay follows a stretched exponential law (Hou et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Experimental study of time response of bending deformation of bone cantilevers in an electric field

Kang

Hou

Qin

2018

Journal of the Mechanical Behavior of Biomedical Materials

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Bone is a complex composite material with hierarchical structures and anisotropic mechanical properties. Bone also processes electromechanical properties, such as piezoelectricity and streaming potentials, which termed as stress generated potentials. Furthermore, the electrostrictive effect and flexoelectric effect can also affect electromechanical properties of the bone. In the present work, time responses of bending deflections of bone cantilever in an external electric field are measured experimentally to investigate bone's electromechanical behavior. It is found that, when subjected to a square waveform electric field, a bone cantilever specimen begins to bend and its deflection increases gradually to a peak value. Then, the deflection begins to decrease gradually during the period of constant voltage. To analyze the reasons of the bending response of bone, additional experiments were performed. Experimental results obtained show the following two features. The first one is that the electric polarization, induced in bone by an electric field, is due to the Maxwell-Wagner polarization mechanism that the polarization rate is relatively slow, which leads to the electric field force acted on a bone specimen increase gradually and then its bending deflections increase gradually. The second one is that the flexoelectric polarization effect that resists the electric force to decrease and then leads to the bending deflection of a bone cantilever decrease gradually. It is concluded that the first aspect refers to the organic collagens decreasing the electric polarization rate of the bone, and the second one to the inorganic component influencing the bone's polarization intensity.

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Biomechanical and biophysical environment of bone from the macroscopic to the pericellular and molecular level

Cited by 48 publications

References 160 publications

Pressure‐induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling

Pressure‐induced mesenchymal stem cell osteogenesis is dependent on intermediate filament remodeling

Bioreactors for tissue engineering: An update

Experimental study of time response of bending deformation of bone cantilevers in an electric field

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