Biomechanical characterization of a fibrinogen–blood hydrogel for human dental pulp regeneration

Piglionico, Sofia Silvia; Varga, Bela; Pall, Orsolya; Romieu, Olivier; Gergely, Csilla; Cuisinier, Frédéric; Levallois, Bernard; Panayotov, Ivan Vladislavov

doi:10.1039/d3bm00515a

Cited by 4 publications

(2 citation statements)

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“…Fibrinogen requires the addition of thrombin for polymerization and since blood contains thrombin, we can just induce blood to mix with the cell-laden fibrinogen. The gel polymerized, as in a published report (Piglionico et al 2023) and in our in vitro tube study.…”

Section: Discussionmentioning

confidence: 64%

“…Fibrin gel has been widely used or proposed as a scaffold for tissue regeneration (Hubbell 2003;Hong and Stegemann 2008;Janmey et al 2009), including its use for cell-based pulp regeneration (Parmar et al 2020;Piglionico et al 2023). Although collagen hydrogel for cell-based pulp regeneration has shown success in large animal models and human trials (Nakashima et al 2017;Zhu et al 2018;Nakashima et al 2019Nakashima et al , 2022, our in vitro data indicated that even after DPSCs had differentiated into EC-like cells, tremendous contraction still occurred in collagen gels (Appendix Figs.…”

Section: Discussionmentioning

confidence: 90%

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Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study

Zhang,

Liu,

de Souza Araujo

et al. 2024

J Dent Res

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The process of neovascularization during cell-based pulp regeneration is difficult to study. Here we developed a tube model that simulates root canal space and allows direct visualization of the vascularization process in vitro. Endothelial-like cells (ECs) derived from guiding human dental pulp stem cells (DPSCs) into expressing endothelial cell markers CD144, vWF, VEGFR1, and VEGFR2 were used. Human microvascular endothelial cells (hMVECs) were used as a positive control. DPSC-ECs formed tubules on Matrigel similar to hMVECs. Cells were mixed in fibrinogen/thrombin or mouse blood and seeded into wells of 96-well plates or injected into a tapered plastic tube (14 mm in length and 1 or 2 mm diameter of the apex opening) with the larger end sealed with MTA to simulate root canal space. Cells/gels in wells or tubes were incubated for various times in vitro and observed under the microscope for morphological changes. Samples were then fixed and processed for histological analysis to determine vessel formation. Vessel-like networks were observed in culture from 1 to 3 d after cell seeding. Cells/gels in 96-well plates were maintained up to 25 d. Histologically, both hMVECs and DPSC-ECs in 96-well plates or tubes showed intracellular vacuole formation. Some cells showed merged large vacuoles indicating the lumenization. Tubular structures were also observed resembling blood vessels. Cells appeared healthy throughout the tube except some samples (1 mm apical diameter) in the coronal third. Histological analysis also showed pulp-like soft tissue throughout the tube samples with vascular-like structures. hMVECs formed larger vascular lumen size than DPSC-ECs while the latter tended to have more lumen and tubular structure counts. We conclude that DPSC-ECs can form vascular structures and sustained in the 3-dimensional fibrin gel system in vitro. The tube model appears to be a proper and simple system simulating the root canal space for vascular formation and pulp regeneration studies.

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Section: Discussionmentioning

confidence: 64%

Section: Discussionmentioning

confidence: 90%

Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study

Zhang,

Liu,

de Souza Araujo

et al. 2024

J Dent Res

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Deconstructing fibrin(ogen) structure

Risman,

Sen,

Tutwiler

et al. 2024

Journal of Thrombosis and Haemostasis

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Dental-derived stem cells in tissue engineering: the role of biomaterials and host response

Yuan,

Ferreira,

et al. 2023

Regenerative Biomaterials

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Dental-derived stem cells (DSCs) are attractive cell sources due to their easy access, superior growth capacity, and low immunogenicity. They can respond to multiple extracellular matrix (ECM) signals, which provide biophysical and biochemical cues to regulate the fate of residing cells. However, the direct transplantation of DSCs suffers from poor proliferation and differentiation towards functional cells and low survival rates due to local inflammation. Recently, elegant advances in the design of novel biomaterials have been made to give promise to the use of biomimetic biomaterials to regulate various cell behaviors, including proliferation, differentiation, and migration. Biomaterials could be tailored with multiple functionalities, e.g., stimuli-responsiveness. There is an emerging need to summarize recent advances in engineered biomaterials-mediated delivery and therapy of DSCs and their potential applications. Herein, we outlined the design of biomaterials for supporting DSCs and the host response to the transplantation.

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Biomechanical characterization of a fibrinogen–blood hydrogel for human dental pulp regeneration

Abstract: In dental practice, Regenerative Endodontic Treatment (RET) is applied as an alternative to classical endodontic treatment of immature necrotic teeth. This procedure, also known as dental pulp revitalization, relies on...

Cited by 4 publications

References 58 publications

Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study

Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study

Deconstructing fibrin(ogen) structure

Dental-derived stem cells in tissue engineering: the role of biomaterials and host response

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