Biomechanical evaluation of the rabbit tibia after implantation of porous hydroxyapatite/collagen in a rabbit model

Masaoka, Tomokazu; Yamada, Takatsugu; Yuasa, Masato; Yoshii, Toshitaka; Okawa, Atsushi; Morita, Sadao; Kozaka, Yuko; Hirano, Minoru; Sotome, Shinichi

doi:10.1016/j.jos.2015.11.008

Cited by 16 publications

(16 citation statements)

References 19 publications

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“…26 On the other hand, the osteoconductivity of porous HAp/Col seems to be much higher than that of calcium sulfate, as indicated by experiments of HAp/Col implanted into non-infected bones. 11,[27][28][29] These results indicate that HAp/Col may have advantages in terms of early bone regeneration.…”

Section: Discussionmentioning

confidence: 83%

“…However, Ferguson reported a 4.6% fracture rate, which suggests that early bone regeneration at the bone infection site might be inadequate with calcium sulfate . On the other hand, the osteoconductivity of porous HAp/Col seems to be much higher than that of calcium sulfate, as indicated by experiments of HAp/Col implanted into non‐infected bones . These results indicate that HAp/Col may have advantages in terms of early bone regeneration.…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of Antibiotic‐Loaded Hydroxyapatite/Collagen Composites Is Dependent on Adsorbability for Treating Staphylococcus aureus Osteomyelitis in Rats

Egawa

Hirai

Matsumoto

et al. 2019

Journal Orthopaedic Research

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Osteomyelitis remains one of the most challenging disorders for orthopedic doctors despite the advancement of therapeutic techniques. The purpose of this study was to investigate the feasibility of local antibiotic administration using hydroxyapatite/collagen (HAp/Col) as a drug delivery system. We hypothesized that higher adsorbability of antibiotics onto HAp/Col will result in more efficacious activity and therefore, treatment of osteomyelitis. Eight antibiotics were examined in this study: amikacin, cefazolin, cefotiam, daptomycin, minocycline, piperacillin, teicoplanin, and vancomycin. Aligning with their adsorbability onto HAp/Col, minocycline, teicoplanin, and vancomycin showed antibacterial effects up to 14 days after subcutaneous implantation in Wistar rats; while antibiotics with reduced adsorbability (cefazolin, cefotiam, piperacillin) had diminished antibacterial effects. Furthermore, when implanted into a rat femur, vancomycin levels from the Hap/Col were detected in the medullary space above the minimum inhibitory concentration for Staphylococcus aureus for 7 days, while cefazolin levels were undetectable. Aligning with these results, implantation of Hap/Col impregnated with vancomycin to the femur in an acute osteomyelitis rat model had a greater therapeutic effect than cefazolin, as measured by the number of bacteria, the extent of bone destruction, and bone regeneration. These results indicated that the adsorbability of antibiotics onto their carrier is important when locally administered and that HAp/Col scaffolds might be a useful antibiotic delivery system for osteomyelitis. © 2019 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of Orthopaedic Research Society J Orthop Res 38:843‐851, 2020

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Efficacy of Antibiotic‐Loaded Hydroxyapatite/Collagen Composites Is Dependent on Adsorbability for Treating Staphylococcus aureus Osteomyelitis in Rats

Egawa

Hirai

Matsumoto

et al. 2019

Journal Orthopaedic Research

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“…Reported examples of such scaffolds include alpha tricalcium phosphate, 20 glycosaminoglucan hydrogel, 29 atelocollagen, 17 and HAp/col. 32 The reasons to use HAp/Col to make bFGF controlled release in this study were that HAp/Col has good absorbency because of its spongiform nature and it is easy to deal with because of its softness.…”

Section: Discussionmentioning

confidence: 99%

Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite

Yamaguchi

Kaji

Nakamura

et al. 2017

J reconstr Microsurg

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Basic fibroblast growth factor (bFGF) is known to stimulate bone formation and angiogenesis. Hydroxyapatite/collagen composite (HAp/Col) is also known to have very strong bone conductive activity. In this study, prefabrication of vascularized allogenic bone (allo-bone) graft was attempted in recipients by implanting vascular bundles from recipients into the transplanted allo-bone graft. Furthermore, the effect of bFGF-containing HAp/Col on the prefabricated vascularized allo-bone graft was investigated. In this study, 32 Sprague-Dawley rats were used as donors, and bone grafts were collected from their femora. Thirty-two Wistar rats (recipients) were divided into four groups, and the allo-bone grafts were transplanted into the thigh region. In the experimental groups, one or both of the flow-through saphenous vascular bundles and 100-μg bFGF-containing HAp/Col were implanted into the medullary cavity of the allo-bone grafts. In the control group, neither was implanted. These rats were sacrificed at 4 weeks after transplantation, and boneformation, angiogenesis, and bone resorption in the transplanted allo-bone grafts were evaluated histologically and genetically. Bone formation and angiogenesis in the transplanted allo-bone graft were effectively stimulated by implanting vascular bundles or bFGF-containing HAp/Col on both histological and genetic evaluations compared with the control group. The most significant stimulation was observed in the group in which both were implanted. Bone resorption was not stimulated in any group. By implanting a flow-through vascular bundle and bFGF-containing HAp/Col, an ideal vascularized allo-bone graft that had high bone formative and angiogenetic activities and did not stimulate bone resorptive activity was prefabricated.

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“…Several studies have been conducted to improve HA ceramics properties. Addition of total blood [ 36 ], platelet rich plasma [ 36 , 37 ], bone morphogenetic proteins (BMPs) [ 16 , 38 ] collagen [ 39 ], mesenchymal stem cells [ 40 ], and BM [ 41 , 42 ] are among the most proposed techniques.…”

Section: Discussionmentioning

confidence: 99%

Repair of segmental radial defect with autologous bone marrow aspirate and hydroxyapatite in rabbit radius: A clinical and radiographic evaluation

Yassine¹,

Mokhtar²,

Hemida³

et al. 2017

Vet World

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Aim:Finding an ideal bone substitute to treat large bone defects, delayed union and nonunions remain a challenge for orthopedic surgeons and researchers. Several studies have been conducted on bone regeneration; each has its own advantages and disadvantages. The aim of this study was to evaluate the effect of a combination of hydroxyapatite (HA) powder with autologous bone marrow (BM) aspirate on the repair of segmental radial defect in a rabbit model.Materials and Methods:A total of 36 male and adult New Zealand rabbit with a mean weight of 2.25 kg were used in this study. Approximately, 5 mm defect was created in the mid-shaft of the radius to be filled with HA powder in the control group “HA” (n=18) and with a combination of HA powder and autologous BM aspirate in the test group “HA+BM” (n=18). Animals were observed daily for healing by inspection of the surgical site, and six rabbits of each group were sacrificed at 30, 60, and 90 post-operative days to perform a radiographic evaluation of defect site.Results:Obtained results revealed a better and more rapid bone regeneration in the test group: Since the defect was rapidly and completely filled with mature bone tissue after 90 days.Conclusion:Based on these findings, we could infer that adding a BM aspirate to HA is responsible of a better regeneration process leading to a complete filling of the defect.

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Biomechanical evaluation of the rabbit tibia after implantation of porous hydroxyapatite/collagen in a rabbit model

Cited by 16 publications

References 19 publications

Efficacy of Antibiotic‐Loaded Hydroxyapatite/Collagen Composites Is Dependent on Adsorbability for Treating Staphylococcus aureus Osteomyelitis in Rats

Efficacy of Antibiotic‐Loaded Hydroxyapatite/Collagen Composites Is Dependent on Adsorbability for Treating Staphylococcus aureus Osteomyelitis in Rats

Prefabrication of Vascularized Allogenic Bone Graft in a Rat by Implanting a Flow-Through Vascular Pedicle and Basic Fibroblast Growth Factor Containing Hydroxyapatite/Collagen Composite

Repair of segmental radial defect with autologous bone marrow aspirate and hydroxyapatite in rabbit radius: A clinical and radiographic evaluation

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