Biomechanical properties of the ex vivo porcine trachea: A benchmark for three-dimensional bioprinted airway replacements

Kaye, Rachel; Cao, Angela; Goldstein, Todd; Grande, Daniel; Zeltsman, David; Smith, Lee P.

doi:10.1016/j.amjoto.2021.103217

Cited by 9 publications

(2 citation statements)

References 31 publications

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“…The tracheal tissue in the longitudinal direction attains 30% strain below 50 kPa while the same level of strain is attained in the circumferential direction at stresses of about 100 kPa (double as much as that in the longitudinal direction). These findings are in line with previous studies by other researchers on the relatively higher stiffness of the tracheal tissue along the circumferential direction (Hoffman et al, 2016;Kaye et al, 2022) as compared to the longitudinal direction. Along the circumferential direction the toe region is only up to 15% strain as opposed to the 30% strain for the circumferential direction.…”

Section: Resultssupporting

confidence: 93%

Experimental analysis and biaxial biomechanical behaviour of ex-vivo sheep trachea

Pandelani,

Ngwangwa,

Nemavhola

2023

Front. Mater.

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Besides surgery, there are currently no other established methods for routine treatment of tracheal pathologies such a tracheal stenosis or tracheal and airway tumors. Even with several attempts to repair the infected trachea with artificial and natural prostheses, there is a need for the fundamental understanding of the tissue’s mechanical behaviour. The purpose of this study was to investigate the mechanical behaviour of the tracheal tissue under biaxial tensile loading. Furthermore, the study examines the material properties of the tissue through a study of the model parameters for six constitutive models. Materials and methods: The fourteen (n = 14) specimens of sheep trachea (Vleis Merino) measured to be ∼30 × 20 mm where only the effective area of ∼25 × 16 mm was subjected to engineering strain. In this study, we assume that the tracheal tissue is anisotropic and incom-pressible, therefore we apply and study the material parameters from six different constitutive material models. Results: The results show that the tracheal tissue is twice as stiff along the circumferential direction as it is along the longitudinal direction. It is also observed that the material properties are different (non-homogeneous) along the trachea. Conclusion: The findings of this study will benefit computational models for the study of tracheal diseases or injuries. Furthermore, these findings will assist in the development of regenerative medicine for different tracheal pathologies and in the bioengineering of replacement tissue in cases of damage.

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Section: Resultssupporting

confidence: 93%

Experimental analysis and biaxial biomechanical behaviour of ex-vivo sheep trachea

Pandelani,

Ngwangwa,

Nemavhola

2023

Front. Mater.

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“…Since Montgomery’s invention of the t-tube silicone stent for tracheal stenosis in 1965, a variety of stents have been developed. Freeze drying [ 21 ], electrostatic spinning [ 22 ], three-dimensional (3D) printing [ 23 , 24 , 25 ], textile techniques [ 26 , 27 ], and many other methods have been used for the manufacture of tracheal stents. Although these manufacturing methods produce stents with 3D porous structures, they are limited in controlling pore size.…”

Section: Introductionmentioning

confidence: 99%

A Review of Woven Tracheal Stents: Materials, Structures, and Application

Chen

Huang

et al. 2022

JFB

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The repair and reconstruction of tracheal defects is a challenging clinical problem. Due to the wide choice of materials and structures, weaving technology has shown unique advantages in simulating the multilayer structure of the trachea and providing reliable performance. Currently, most woven stent-based stents focus only on the effect of materials on stent performance while ignoring the direct effect of woven process parameters on stent performance, and the advantages of weaving technology in tissue regeneration have not been fully exploited. Therefore, this review will introduce the effects of stent materials and fabric construction on the performance of tracheal stents, focusing on the effects of weaving process parameters on stent performance. We will summarize the problems faced by woven stents and possible directions of development in the hope of broadening the technical field of artificial trachea preparation.

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A Clinical‐Grade Partially Decellularized Matrix for Tracheal Replacement: Validation In Vitro and In Vivo in a Porcine Model

Arakelian,

Léger,

Kellouche

et al. 2024

Advanced Biology

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The management of extensive tracheal resection followed by circumferential replacement remains a surgical challenge. Numerous techniques are proposed with mixed results. Partial decellularization of the trachea with the removal of the mucosal and submucosal cells is a promising method, reducing immunogenicity while preserving the biomechanical properties of the final matrix. Despite many research protocols and proofs of concept, no standardized clinical grade protocol is described. Furthermore, local and systemic biointegration mechanisms of decellularized trachea are not well known. Therefore, in a translational research perspective, this work set up a partial tracheal decellularization protocol in line with Cell and Tissue Products regulations. Extensive characterization of the final product is performed in vitro and in vivo. The results show that the Partially Decellularized Trachea (PDT) is cell‐free in the mucosa and submucosa, while the cartilage structure is preserved, maintaining the biomechanical properties of the trachea. When implanted in the muscle in vivo for 28 days, no systemic inflammation is observed, and locally, the PDT shows an excellent biointegration and vascularization. No signs of graft rejection are observed. These encouraging results confirmed the efficacy of the clinical grade PDT production protocol, which is an important step for future clinical applications.

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Biomechanical properties of the ex vivo porcine trachea: A benchmark for three-dimensional bioprinted airway replacements

Cited by 9 publications

References 31 publications

Experimental analysis and biaxial biomechanical behaviour of ex-vivo sheep trachea

Experimental analysis and biaxial biomechanical behaviour of ex-vivo sheep trachea

A Review of Woven Tracheal Stents: Materials, Structures, and Application

A Clinical‐Grade Partially Decellularized Matrix for Tracheal Replacement: Validation In Vitro and In Vivo in a Porcine Model

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