Biomedical Applications of Selected Ion Monitoring

Falkner, Fred C.; Sweetman, Brian J.; Watson, J. Throck

doi:10.1080/05704927508085062

Cited by 53 publications

(10 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The good agreement gives added confidence in the RIA, as of the various procedures available for the analysis of indoles and related compounds, those based on selected ion monitoring procedures are undoubtedly the most specific and reliable (8,9). This technique has now been successfully applied to the detection of melatonin in a variety of biological extracts (2,3,15), and the PFP ester employed in the present study established as the derivative of choice (15).…”

Section: Discussionmentioning

confidence: 88%

See 1 more Smart Citation

A Specific Radioimmunoassay for Melatonin in Biological Tissue and Fluids and its Validation by Gas Chromatography-Mass Spectrometry

Kennaway¹,

Frith²,

Phillipou³

et al. 1977

Endocrinology

207

View full text Add to dashboard Cite

A specific practical radioimmunoassay suited to determinations of melatonin in both tissues and body fluids is described. The rabbit antibody employed was raised to an antigen formed by condensation between N-acetylserotonin and the Mannich adduct of bovine serum albumin and formaldehyde. Substitution was shown by protonmagnetic resonance spectroscopy to occur exclusively at die 4 position of the indole nucleus. The antibody reacted with a variety of N-acetylated indoles, and absolute specificity was dependent upon the extraction procedure and column (Lipidex 5000) chromatography.In addition to the usual reliability criteria, the validity of the assay was checked by gas chromatography-mass spectrometry using [ 2 H 3 ]melatonin as an internal standard, the preparation of which is described. The occurrence of melatonin in the plasma of man, sheep, rat and chicken was confirmed, and its presence in the plasma of the pig (22-76 pg/ml), donkey (24-128 pg/ml), cow (20-320 pg/ml), camel (29-221 pg/ml) and a scincid lizard (20-500 pg/ml) established. A nocturnal rise in plasma melatonin content occurred in all species.Melatonin was found in the plasma of ewes 2-12 weeks after pinealectomy, but the nocturnal rise was abolished. The results establish a nyctohemeral variation in plasma melatonin in a wide variety of species, and indicate that sources of melatonin other than the pineal may assume precedence following pinalectomy. (Endocrinology 101: 119, 1977)

show abstract

Section: Discussionmentioning

confidence: 88%

“…tions of melatonin in both tissues and body fluids on a routine basis. In addition to the usual criterion of reliability, the method is validated by the technique of selected ion monitoring (8,9). Its application to determination of the plasma melatonin content of a variety of species kept under diurnal lighting conditions is reported.…”

mentioning

confidence: 99%

A Specific Radioimmunoassay for Melatonin in Biological Tissue and Fluids and its Validation by Gas Chromatography-Mass Spectrometry

Kennaway¹,

Frith²,

Phillipou³

et al. 1977

Endocrinology

207

View full text Add to dashboard Cite

show abstract

“…The use of stable isotope-labelled derivatives as carriers for the identification and quanti-tation of small amounts of the corresponding unlabelled compound by mass spectrometry and CC-MS is a well established technique [7] , particularly in metabolism studies. Nevertheless, we believe this to be the first reported example of the application of this technique to the identification and quantitation of endogenous phytohormones.…”

Section: Resultsmentioning

confidence: 99%

The occurrence of raphanatin as an endogenous cytokinin in radish seed Identification and quantitation by gas chromatographic—mass spectrometric analysis using deuterium‐labelled standards

et al. 1977

View full text Add to dashboard Cite

“…4 The labeled analog, usually introduced in large excess, serves both as an internal carrier and a The method of continuous reference compound admission (method I) is widely applicable but suffers from the potential disadvantage of interference between reference and sample compound peaks, especially when used under low resolution conditions. Moreover, it is necessary to know the flow rate of the reference compound in absolute terms, and the relative mass spectral sensitivities of the reference and standard samples.…”

Section: Introductionmentioning

confidence: 99%

Some considerations on quantitative methodology and detection limits in organic mass spectrometry

Marshall¹,

Petersen

Vouros

1978

Biol. Mass Spectrom.

View full text Add to dashboard Cite

Mass spectrometric sensitivity data for the compounds cholesterol, cholestane, DDT, decafluorotriphenylphosphine, morphine, LSD and methyl stearate have been developed over a period of time and under a variety of conditions. This information is presented on both a relative and an absolute basis. With the exception of morphine, relative sensitivies were reproducible to within a factor of three over a six month period. This reproducibility facilitates quantitation and a single standard can be used for calibration of organic compounds from various classes. Detection limits of 50 femtograms for LSD are realized for samples introduced via the direct inlet probe.

show abstract

Biomedical Applications of Selected Ion Monitoring

Cited by 53 publications

References 83 publications

A Specific Radioimmunoassay for Melatonin in Biological Tissue and Fluids and its Validation by Gas Chromatography-Mass Spectrometry

A Specific Radioimmunoassay for Melatonin in Biological Tissue and Fluids and its Validation by Gas Chromatography-Mass Spectrometry

The occurrence of raphanatin as an endogenous cytokinin in radish seed Identification and quantitation by gas chromatographic—mass spectrometric analysis using deuterium‐labelled standards

Some considerations on quantitative methodology and detection limits in organic mass spectrometry

Contact Info

Product

Resources

About