Biomedical Imaging in Experimental Models of Cardiovascular Disease

Sosnovik, David E.; Scherrer‐Crosbie, Marielle

doi:10.1161/circresaha.122.320306

Cited by 8 publications

(6 citation statements)

References 173 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An initial step toward effective therapy includes understanding of disease progression, and a plethora of techniques are available for probing orientation of the immune environment post-MI and assessing functional outcomes. With regard to assessment of LV remodeling, biomedical imaging techniques are widely used in experimental models of CVD and have been recently reviewed elsewhere [ 114 , 115 ]. These techniques include structural, functional, and biochemical readouts by a variety of techniques that include angiography, echocardiography, MRI, positron emission tomography (PET), CT, and fluorescence imaging.…”

Section: Experimental Models and Assessment Of Inflammatory Pathophys...mentioning

confidence: 99%

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Soni

D'Elia

Rodell

2023

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

Ischemic heart failure (IHF) is a leading cause of morbidity and mortality worldwide, for which heart transplantation remains the only definitive treatment. IHF manifests from myocardial infarction (MI) that initiates tissue remodeling processes, mediated by mechanical changes in the tissue (loss of contractility, softening of the myocardium) that are interdependent with cellular mechanisms (cardiomyocyte death, inflammatory response). The early remodeling phase is characterized by robust inflammation that is necessary for tissue debridement and the initiation of repair processes. While later transition toward an immunoregenerative function is desirable, functional reorientation from an inflammatory to reparatory environment is often lacking, trapping the heart in a chronically inflamed state that perpetuates cardiomyocyte death, ventricular dilatation, excess fibrosis, and progressive IHF. Therapies can redirect the immune microenvironment, including biotherapeutic and biomaterial-based approaches. In this review, we outline these existing approaches, with a particular focus on the immunomodulatory effects of therapeutics (small molecule drugs, biomolecules, and cell or cell-derived products). Cardioprotective strategies, often focusing on immunosuppression, have shown promise in pre-clinical and clinical trials. However, immunoregenerative therapies are emerging that often benefit from exacerbating early inflammation. Biomaterials can be used to enhance these therapies as a result of their intrinsic immunomodulatory properties, parallel mechanisms of action (e.g., mechanical restraint), or by enabling cell or tissue-targeted delivery. We further discuss translatability and the continued progress of technologies and procedures that contribute to the bench-to-bedside development of these critically needed treatments. Graphical Abstract

show abstract

Section: Experimental Models and Assessment Of Inflammatory Pathophys...mentioning

confidence: 99%

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Soni

D'Elia

Rodell

2023

Drug Deliv. and Transl. Res.

View full text Add to dashboard Cite

show abstract

“…where C a , C m , and C v are the H For convenience, the unit for C m is chosen as μmol/(g heart tissue water), and the units for C a and C v are μmol/(g blood water), where parameters f 1 (=1.266) and f 2 (=1.077) are conversion factors used for consistency of units applied in Eq. (1). Because the tissue water composition is similar between brain and heart, 17 the same f 1 and f 2 conversion factors can be applied to brain and heart tissues.…”

Section: F I G U R Ementioning

confidence: 99%

“…With technological advancements over the past few decades, many biomedical imaging tools are available to noninvasively study different aspects of cardiac pathophysiology and function in animal models and humans (please see recent reviews on this topic 1,2 and the references cited therein). Of these, techniques based on MRS or MRSI and PET are the most relevant modalities for cardiometabolic imaging.…”

Section: Introductionmentioning

confidence: 99%

Quantitative ¹⁷O MRSI of myocardial oxygen metabolic rate, blood flow, and oxygen extraction fraction under normal and high workload conditions

Zhu,

Chen

2023

Magnetic Resonance in Med

View full text Add to dashboard Cite

PurposeThe heart is a highly aerobic organ consuming most of the oxygen the body in supporting heart function. Quantitative imaging of myocardial oxygen metabolism and perfusion is essential for studying cardiac physiopathology in vivo. Here, we report a new imaging method that can simultaneously assess myocardial oxygen metabolism and blood flow in the rat heart.MethodsThis novel method is based on the 17O‐MRSI combined with brief inhalation of 17O‐isotope labeled oxygen gas for quantitative imaging of myocardial metabolic rate of oxygen consumption (MVO2), myocardial blood flow (MBF), and oxygen extraction fraction (OEF). We demonstrate this imaging method under basal and high workload conditions in rat hearts at 9.4 T.ResultsWe show that this 17O MRSI–based approach can directly measure and image MVO2 (1.35–4.06 μmol/g/min), MBF (0.49–1.38 mL/g/min), and OEF (0.33–0.44) in the heart of anesthetized rat under basal and high workload (21.6 × 103–56.7 × 103 mmHg • bpm) conditions. Under high workload condition, MVO2 and MBF values in healthy rats approximately doubled, whereas OEF remained unchanged, indicating a strong coupling between myocardial oxygen metabolic demand and supply through blood perfusion.ConclusionThe 17O‐MRSI method has been used to simultaneously image the myocardial metabolic rate of oxygen consumption, blood flow, and oxygen extraction fraction in small animal hearts, which are sensitive to the physiological changes induced by high workload. This approach could provide comprehensive measures that are critical for studying myocardial function in normal and diseased states and has a potential for translation.

show abstract

“…In vivo molecular imaging is an advanced imaging approach that combines biochemical and physiological changes occurring in living animals at the molecular level [1,2]. Fluorescence [3,4], luminescence [5,6], singlephoton emission computed tomography (SPECT) [7], positron emission tomography (PET) [8,9], computed tomography (CT) [10,11], and magnetic resonance imaging (MRI) are techniques used to obtain images [12,13].…”

Section: Introductionmentioning

confidence: 99%

In vivo molecular imaging in preclinical research

Kim

Lee

2022

Lab Anim Res

View full text Add to dashboard Cite

In vivo molecular imaging is a research field in which molecular biology and advanced imaging techniques are combined for imaging molecular-level biochemical and physiological changes that occur in a living body. For biomolecular imaging, the knowledge of molecular biology, cell biology, biochemistry, and physiology must be applied. Imaging techniques such as fluorescence, luminescence, single-photon emission computed tomography (SPECT), positron emission tomography (PET), computed tomography (CT), and magnetic resonance imaging (MRI) are used for biomolecular imaging. These imaging techniques are used in various fields, i.e., diagnosis of various diseases, development of new drugs, development of treatments, and evaluation of effects. Moreover, as biomolecular imaging can repeatedly acquire images without damaging biological tissues or sacrificing the integrity of objects, changes over time can be evaluated.Phenotypes or diseases in a living body are caused by the accumulation of various biological phenomena. Genetic differences cause biochemical and physiological differences, which accumulate and cause anatomical or structural changes. Biomolecular imaging techniques are suitable for each step. In evaluating anatomical or structural changes, MRI, CT, and ultrasound have advantages in obtaining high-resolution images. SPECT and MRI are advantageous for the evaluation of various physiological phenomena. PET and magnetic resonance spectroscopy can be used to image biochemical phenomena in vivo. Although various biomolecular imaging techniques can be used to evaluate various biological phenomena, it is important to use imaging techniques suitable for each purpose.

show abstract

Biomedical Imaging in Experimental Models of Cardiovascular Disease

Cited by 8 publications

References 173 publications

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Quantitative ¹⁷O MRSI of myocardial oxygen metabolic rate, blood flow, and oxygen extraction fraction under normal and high workload conditions

In vivo molecular imaging in preclinical research

Contact Info

Product

Resources

About

Biomedical Imaging in Experimental Models of Cardiovascular Disease

Cited by 8 publications

References 173 publications

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Control of the post-infarct immune microenvironment through biotherapeutic and biomaterial-based approaches

Quantitative 17O MRSI of myocardial oxygen metabolic rate, blood flow, and oxygen extraction fraction under normal and high workload conditions

In vivo molecular imaging in preclinical research

Contact Info

Product

Resources

About

Quantitative ¹⁷O MRSI of myocardial oxygen metabolic rate, blood flow, and oxygen extraction fraction under normal and high workload conditions