Biomedical potential of silver nanoparticles synthesized from calli cells of Citrullus colocynthis (L.) Schrad

Abstract

BackgroundAn increasingly common application is the use of silver nanoparticles for antimicrobial coatings, wound dressings, and biomedical devices. In this present investigation, we report, biomedical potential of silver nanopaticles synthesized from calli extract of Citrullus colocynthis on Human epidermoid larynx carcinoma (HEp -2) cell line.MethodsThe callus extract react with silver nitrate solution confirmed silver nanoparticles synthesis through the steady change of greenish colour to reddish brown and characterized by using FT-IR, AFM. Toxicity on HEp 2 cell line assessed using MTT assay, caspase -3 assay, Lactate dehydrogenase leakage assay and DNA fragmentation assay.ResultsThe synthesized silver nanoparticles were generally found to be spherical in shape with size 31 nm by AFM. The molar concentration of the silver nanoparticles solution in our present study is 1100 nM/10 mL. The results exhibit that silver nanoparticles mediate a dose-dependent toxicity for the cell tested, and the silver nanoparticles at 500 nM decreased the viability of HEp 2 cells to 50% of the initial level. LDH activities found to be significantly elevated after 48 h of exposure in the medium containing silver nanoparticles when compared to the control and Caspase 3 activation suggested that silver nanoparticles caused cell death through apoptosis, which was further supported by cellular DNA fragmentation, showed that the silver nanoparticles treated HEp2 cells exhibited extensive double strand breaks, thereby yielding a ladder appearance (Lane 2), while the DNA of control HEp2 cells supplemented with 10% serum exhibited minimum breakage (Lane 1). This study revealed completely would eliminate the use of expensive drug for cancer treatment.

Preparation, Characterization and <i>in Vitro</i> Evaluation of the Antitumor Activity of the Biologically Synthesized Silver Nanoparticles

“…In agreement with the present finding, a work done by Satyavani and colleagues revealed that AgNPs induced cell death via activation of caspase-3 activity and DNA fragmentation which are both apoptotic events [42]. The increased level of caspase-3 activity was observed in human breast cancer cells MDA-MB-231 in response to the cytotoxic potential of the biologically synthesized AgNPs treatment [21].…”

Microwave-Assisted Green Synthesis and Characterization of Silver Nanoparticles Using Melia azedarach for the Management of Fusarium Wilt in Tomato

“…The results of the current investigation are in accordance to previous studies (Raghunandan et al, 2011; that suggested that the peak from 1,607 to 1,636 cm −1 is due to C=C aromatic ring stretching vibrations. However, Satyavani et al (2011) proposed that the peaks at 1,620-1,636 cm −1 represent carbonyl group (C=O) present in polyphenols such as theaflavin, epicatechin gallate, and catechin gallate. The major sources for these vibration bands are various phytochemicals, flavonoids, and alkaloids that are copiously present in the leaf.…”

Efficiency of Biosynthesized Silver and Zinc Nanoparticles Against Multi-Drug Resistant Pathogens

“…The absorbance of colored solution was measured on the Multimode reader (Synergy HTX Multimode reader, BIOTEK, India) using a test wavelength of 490 nm (Freshney, 2010; Satyavani et al, 2011). Percentage toxicity was estimated for each concentration, and the IC 50 value was calculated for both nanoparticles (Freshney, 2005).…”