Biometric and biochemical attributes of alfalfa seedlings as indicators of stress induced by excessive cadmium

Mahmood, Seema; Malik, Saeed Ahmad; Tabassum, Arooma; Younis, Uzma; Athar, Mohammad

doi:10.4067/s0718-95162014005000043

Cited by 6 publications

(6 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other studies also found a Cd-induced decrease in chlorophyll content in plants of Eucalyptus camaldulensis (MARQUES et al, 2011), Pisum sativum L. (JANUŠKAITIEN, 2012) and Medicago sativa L. (MAHMOOD et al, 2014).…”

Section: Resultsmentioning

confidence: 85%

Photosynthetic Parameters as Physiological Indicators of Tolerance to Cadmium Stress in Sunflower Genotypes

et al. 2018

View full text Add to dashboard Cite

The objective of the present study was to evaluate the tolerance to cadmium (Cd) of sunflower genotypes grown in greenhouse conditions, and the effectiveness of using photosynthetic parameters as physiological indicators of this tolerance. Seeds of two sunflower genotypes previously identified as tolerant (H358) and Cd-sensitive (AG960) to Cd were used. The seeds were germinated in plastic cups containing plant substrate; after 9 days, the seedlings were transplanted to plastic basins containing a nutrient solution with 0 or 10 µM of Cd, where they remained for 16 days. Samples of the plants were harvested every 5 days. The experiment was carried out in a randomized complete design, using a 4×2×2 factorial arrangement (4 days of grown in a nutrient solution with Cd, 2 sunflower genotypes, and 2 Cd levels) with four replications. Cd stress decreased CO 2 net assimilation, stomatal conductance, carboxylation efficiency, photosynthetic pigment contents, potential quantum yield (F v /F m ) , and effective quantum yield of plants of the two evaluated genotypes. The decrease in photosynthetic rates of these plants was caused by both stomatal and non-stomatal limitations. Plants of the AG960 genotype showed more pronounced deleterious effects due to Cd stress than those of the H358 genotype. Thus, CO 2 net assimilation rate, stomatal conductance, and chloroplast pigment content are good physiological indicators of sunflower tolerance to Cd and can at least in part, explain the greater tolerance of the H358 genotype to Cd stress when compared to the AG960 genotype. EFICÁCIA DOS PARÂMETROS FOTOSSINTÉTICOS COMO INDICADORES FISIOLÓGICOS DA TOLERÂNCIA AO CÁDMIO EM GIRASSOLRESUMO -O presente estudo foi conduzido em casa de vegetação objetivando-se demonstrar a tolerância ao cádmio (Cd) em genótipos de girassol e avaliar a eficácia dos parâmetros fotossintéticos como indicadores fisiológicos dessa tolerância. Para isto, foram utilizadas sementes de dois genótipos de girassol previamente identificados como tolerante (H358) e sensível (AG960) ao Cd.As sementes foram germinadas em copos plásticos contendo substrato vegetal e, após 9 dias, as plântulas foram transferidas para bacias plásticas com solução nutritiva contendo 0 ou 10 µM de Cd , onde permaneceram por 16 dias. As plantas foram coletadas a cada 5 dias. O delineamento experimental foi o inteiramente casualizado, em um arranjo fatorial 4 (dias de exposição) × 2 (genótipos) × 2 (níveis de Cd), com quatro repetições. A exposição ao Cd reduziu a taxa de assimilação líquida do CO 2 , a condutância estomática e a eficiência de carboxilação, bem como os teores de todos os pigmentos fotossintéticos, a razão F v /F m e o Y II das plantas de ambos os genótipos estudados. Demonstrando que a redução na taxa fotossintética dessas plantas foi ocasionada tanto por limitações estomáticas como não-estomáticas. Os efeitos deletérios do Cd foram mais pronunciados no genótipo AG960 em comparação com o H358. Assim, as variáveis A, gs e os teores de pigmentos mostraram-se bons indicadores fis...

show abstract

Section: Resultsmentioning

confidence: 85%

Photosynthetic Parameters as Physiological Indicators of Tolerance to Cadmium Stress in Sunflower Genotypes

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, the response was found nonsignificant at 600 and 900 µ M-CdCl 2 as compared to controls. The proline content was increased in Brassica juncea , Groenlandia densa , and Medicago sativa as the Cd concentration increased in the treatment [ 52 – 54 ]. Total chlorophyll content was decreased in the leaf of P. dactylifera plant under Cd stress in a dose dependent manner in relation to controls ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Biochemical and Genetical Responses ofPhoenix dactyliferaL. to Cadmium Stress

Al‐Qurainy

Khan

Tarroum

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

The cadmium (Cd), a heavy metal, causes toxicity, which leads to hampering the growth and development of the plant. The molecular and biochemical approaches were used for the investigation of antioxidant system response and genotoxicity in date palm (Phoenix dactylifera L.) cv. Sagai in pot experiment having Cd. The root length was more affected than the shoot length as more accumulation of Cd occurs in roots. Fresh weights of root and shoot were reduced significantly in treated plants as compared to the control. The proline content was increased at low concentration of Cd (300 µM-CdCl2) than the medium and high concentrations (600 and 900 µM-CdCl2), respectively. The thiobarbituric acid reactive substances (TBARS) content was increased at 600 and 900 µM-CdCl2 compared to the plants treated at 300 µM-CdCl2 and controls. Antioxidant enzymatic assay was performed under Cd stress and compared with control plants. The catalase (CAT) and superoxide dismutase (SOD) activities were found to be high in plants treated with CdCl2 at 300 µM compared to at 600 and 900 µM-CdCl2, respectively. The genotoxicity of Cd was assessed using the inter-simple sequence repeat (ISSR) marker where all treated and control plants were clustered into three main groups based on genetic similarity. P. dactylifera plants were found to be more divergent at high Cd stress as compared to control and plants treated at low concentration of Cd.

show abstract

“…Cd is a non-essential nutrient for growth of plants but in-spite of that reality it become accumulated in many crops via roots nutrients uptake channel. This high intake and accumulation of Cd reduced the photosynthesis activity and transpiration (Bazzaz et al, 1974;Mahmood et al, 2014). It also tremendously increases the respiration rate in many plants (Lamoreaux and Chaney, 1978;Lee et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

Drought impact on Pb/Cd toxicity remediated by biochar in Brassica campestris

Fiaz

Danish

Younis

et al. 2014

J. Soil Sci. Plant Nutr.

Self Cite

View full text Add to dashboard Cite

Heavy metals toxicity in the human being is creating an alarming condition in the world. Not only are these metals largely effecting the growth of many plants but also the consumer's health. In sector of agriculture for many years Pakistan is facing the reduction in availability of edible oil. The demand is fulfilled by importation by spending huge cost. Brassica campestris L. is very important oil seed crop of Pakistan that is providing 31% of total oil seed production in Pakistan. But due to toxicity of metals like Cd and Pb its growth and yield is decreasing. Keeping in view the importance of Brassica campestris L. in Pakistan an experiment was conducted using Pollutary manure biochar as a reclaiming agent for heavy metals and an amendment as drought stress reducer to check the improvement in yield of Brassica campestris L. It is found that biochar application not only gave positive correlation in immobilization of Pb and Cd but also improves the plants growth and yield. Enhancements in the Photosynthetic and Accessory pigments are observed regarding good vegetative growth. Thus biochar can be utilized in reducing the metals uptake as well as growth promoter.

show abstract

Biometric and biochemical attributes of alfalfa seedlings as indicators of stress induced by excessive cadmium

Cited by 6 publications

References 28 publications

Photosynthetic Parameters as Physiological Indicators of Tolerance to Cadmium Stress in Sunflower Genotypes

Photosynthetic Parameters as Physiological Indicators of Tolerance to Cadmium Stress in Sunflower Genotypes

Biochemical and Genetical Responses ofPhoenix dactyliferaL. to Cadmium Stress

Drought impact on Pb/Cd toxicity remediated by biochar in Brassica campestris

Contact Info

Product

Resources

About