Biomimetic 3D printed scaffolds for meniscus tissue engineering

Szojka, Alexander R A; Lalh, Karamveer; Andrews, Stephen H. J.; Jomha, Nadr M.; Osswald, Martin; Adesida, Adetola B

doi:10.1016/j.bprint.2017.08.001

Cited by 89 publications

(74 citation statements)

References 29 publications

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“…The meniscus was laser scanned (Handy SCAN 700, Creaform, Canada) and the data were imported into process software (Vxelements, Creaform, Canada) to reconstruct three-dimensional (3D) model. The print parameters of a 3D bioprinter (BIOPLATFORM, Medprint, China) were adjusted (Table 1) as described in a previous study [6], and poly(e-caprolactone) (PCL) (Changchun SinoBiomaterials, China) wire (43-50 kDa) was melted and extruded through a heated metal nozzle to print the scaffold. Simultaneously, the same printing parameters were used to print cylindrical scaffolds, with 5 mm diameter and 3 mm thickness, for the cell compatibility tests of the scaffold.…”

Section: Fabrication Of 3d Printed Scaffoldsmentioning

confidence: 99%

“…The main steps involved in meniscus tissue engineering are preparing a scaffold and seeding cells and regulating the cell-scaffold construct through cytokines, mechanical stimulation, and other methods to synthesize the extracellular matrix (ECM) in vitro, followed by its transplantation in vivo for meniscus regeneration and function [4]. 3D printing technology can fabricate scaffolds with complete control of size, shape, and porosity; it has been used in many previous studies to prepare tissue-engineered meniscus scaffolds [5][6][7][8]. Bone marrow mesenchymal stem cells (BMSCs) are easy to isolate and proliferate, have low immunogenicity, and potential to differentiate into cartilage; thus, they have become ideal seed cell for meniscus tissue engineering [9,10].…”

Section: Introductionmentioning

confidence: 99%

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Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

Zhao

Zou

Cui

et al. 2020

Preprint

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Abstract Background: Cell-based tissue engineering represents a promising management for meniscus repair and regeneration. The present study aimed to investigate whether the injection of parathyroid hormone (PTH) (1-34) could promote the regeneration and chondroprotection of 3D printed scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in a canine total meniscal meniscectomy model. Methods: 3D printed poly(e-caprolactone) scaffold seeded with BMSCs was cultured in vitro, and the effects of in vitro culture time on cell growth and matrix synthesis of the BMSCs-scaffold construct were evaluated by microscopic observation and cartilage matrix content detection at 7, 14, 21, and 28 days. After that, the tissue-engineered meniscus based on BMSCs–scaffold cultured for the appropriate culture time was selected for in vivo implantation. Sixteen dogs were randomly divided into four groups: PTH + BMSCs–scaffold, BMSCs–scaffold, total meniscectomy, and sham operation. The regeneration of the implanted tissue and the degeneration of articular cartilage were assessed by gross, histological, and immunohistochemical analysis at 12 weeks postoperatively.Results: In vitro study showed that the glycosaminoglycan (GAG)/DNA ratio and the expression of collagen type II (Col2) were significantly higher on day 21 as compared to the other time points. In vivo study showed that, compared with the BMSCs–scaffold group, the PTH + BMSCs–scaffold group showed better regeneration of the implanted tissue and greater similarity to native meniscus concerning gross appearance, cells composition, and cartilage extracellular matrix deposition. This group also showed less expression of terminal differentiation markers of BMSC chondrogenesis as well as lower cartilage degeneration with less damage on the knee cartilage surface, higher expression of Col2, and lower expression of degeneration markers.Conclusions: Our results demonstrated that PTH (1-34) promotes the regenerative and chondroprotective effects of the BMSCs–3D printed meniscal scaffold in a canine model, and thus their combination could be a promising strategy for meniscus tissue engineering.

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Section: Fabrication Of 3d Printed Scaffoldsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

Zhao

Zou

Cui

et al. 2020

Preprint

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“…Polycaprolactone has been used as a scaffolding material to form an exact meniscal replica using a 3D printer [102][103][104][105]. MRI scans of the meniscus are converted into a 3D image, data from this image is then used to drive a 3D printer, which produces a scaffold in the exact shape of the meniscus, down to a resolution of 10 μm.…”

Section: D Printing Of Knee Joint Meniscimentioning

confidence: 99%

Novel Approaches in Meniscal Repair Utilizing Mesenchymal Stem Cells, New Generation Bioscaffolds and Biological Adhesives as Cell Delivery Vehicles

Melrose¹

2019

Meniscus of the Knee - Function, Pathology and Management

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Mesenchymal stem cells (MSCs) have been widely applied in the repair of the knee-joint menisci which have a limited ability to undergo spontaneous repair. The menisci stabilise the knee-joint and are weight-bearing structures subjected to considerable tensional and compressive forces during flexion-extension and torsional loading of the knee. Traumatic loading of the knee-joint menisci can generate a number of lesions in the inner avascular meniscal regions. These have a limited capability of intrinsic repair and predispose the underlying articular cartilages to premature osteoarthritis. A number of strategies have therefore been developed for meniscal repair employing MSCs, bioscaffolds, hydrogels, biological glue cell delivery systems and agents which promote cell proliferation/matrix synthesis. Meniscal implants have also been developed in combination with the above procedures. It is important that meniscal defects be repaired not only to maintain kneejoint stability but also to prevent further degenerative changes in other knee joint tissues. Degenerative menisci contribute degradative proteinases and inflammatory mediators to the total synovial degradative proteinase pool. Partial or total surgical removal of the menisci is not a solution since this leads to premature osteoarthritis. Meniscal integrity needs to be maintained or repair strategies implemented in a timely manner to maintain knee joint function.

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“…Despite the advancements in enhancing meniscus healing and regeneration using bioresorbable scaffolds, the challenges to control implant resorption and maintain durability persist. Biomimetic and microsphere scaffolds contain bio‐informed materials that are used to stimulate native cells in vivo to restore zone‐specific meniscal tissue . Current efforts focus on stimulating cells and other seeded biomaterials before their application.…”

Section: Tissue Engineeringmentioning

confidence: 99%

“…Biomimetic and microsphere scaffolds contain bio-informed materials that are used to stimulate native cells in vivo to restore zone-specific meniscal tissue. 191,192 Current efforts focus on stimulating cells and other seeded biomaterials before their application. Strategies to address and improve these issues are currently under investigation.…”

Section: Tissue Engineeringmentioning

confidence: 99%

Current perspectives on biological approaches for osteoarthritis

Whitney

Liebowitz

Bolia

et al. 2017

Annals of the New York Academy of Sciences

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Musculoskeletal injuries that disrupt the structure and function of diarthrodial joints can cause permanent biomechanical alterations and lead to a more severe, chronic condition. Despite advancements that have been made to restore tissue function and delay the need for joint replacement, there are currently no disease-modifying therapies for osteoarthritis (OA). To reduce the risk of OA, innovative preventive medicine approaches have been developed over the last decade to treat the underlying pathology. Several biological approaches are promising treatment modalities for various stages of OA owing to their minimally invasive nature and actively dynamic physiological mechanisms that attenuate tissue degradation and inflammatory responses. Individualized growth factor and cytokine therapies, tissue-engineered biomaterials, and cell-based therapies have revolutionary potential for orthopedic applications; however, the paucity of standardization and categorization of biological components and their counterparts has made it difficult to determine their clinical and biological efficacy. Cell-based therapies and tissue-engineered biologics have become lucrative in sports medicine and orthopedics; nonetheless, there is a continued effort to produce a biological treatment modality tailored to target intra-articular structures that recapitulates tissue function. Advanced development of these biological treatment modalities will potentially optimize tissue healing, regeneration, and joint preservation strategies. Therefore, the purpose of this paper is to review current concepts on several biological treatment approaches for OA.

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Biomimetic 3D printed scaffolds for meniscus tissue engineering

Cited by 89 publications

References 29 publications

Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

Parathyroid hormone (1-34) promotes the effects of 3D printed scaffold-seeded bone marrow mesenchymal stem cells on meniscus regeneration

Novel Approaches in Meniscal Repair Utilizing Mesenchymal Stem Cells, New Generation Bioscaffolds and Biological Adhesives as Cell Delivery Vehicles

Current perspectives on biological approaches for osteoarthritis

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