Biomimetic biohybrid nanofibers containing bovine serum albumin as a bioactive moiety for wound dressing

Homaeigohar, Shahin; Monavari, Mahshid; Koenen, Benedict; Boccaccını, Aldo R.

doi:10.1016/j.msec.2021.111965

Cited by 26 publications

(33 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Incorporating peptides such as IKVAV or YIGSR will likely improve the hydrophilicity of the surface and thus enhance the interactions with cell surface receptors. Given the fact that the cell membrane is negatively charged and some peptide possesses positively charged [ 33 ], further enhancing the cell adhesion properties of the polymeric substrates [ 34 ]. Water contact angles were measured on PLB-g-IKVAV and PLLA fibers and it was shown ( Figure 3 ) that PLB-g-IKVAV fibers become more hydrophilic since the contact angle decreased.…”

Section: Resultsmentioning

confidence: 99%

Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth

Qiang

Wei

Zhou

et al. 2021

Gels

View full text Add to dashboard Cite

Developing scaffolds with appropriate mechanical/structural features as well as tunable bioactivities are indispensable in the field of tissue engineering. This study focused on one such attempt to electrospin the copolymer of L-lactic acid (L-LA) and functional monomer (3(S)- [(benzyloxycarbony)methyl]-1,4-dioxane-2,5-dione, BMD) with small peptide modifications for the purpose of neural tissue engineering. Scanning Electron Microscopy (SEM) micrographs showed fabricated electrospun copolymer as porous and uniform nanofibrous materials with diameter in the range of 800–1000 nm. In addition, the modified scaffolds displayed a lower contact angle than poly(L-lactide) (PLLA) indicating higher hydrophilicity. To further incorporate the bioactive functions, the nanofibers were chemically coupled with small peptide (isoleucine-lysine-valine-alanine-valine, IKVAV). The incorporation of IKVAV onto the electrospun fiber was confirmed by X-ray photoelectron spectroscopy (XPS) and such incorporation did not affect the surface morphology or fiber diameters. To demonstrate the potential of applying the designed scaffolds for nerve regeneration, dorsal root ganglion (DRG) neurons were cultured on the nanofibers to examine the impact on neurite outgrowth of DRGs. The results indicated that the fabricated nanofibrous matrix with small peptide might be a potential candidate for neural tissue engineering.

show abstract

Section: Resultsmentioning

confidence: 99%

Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth

Qiang

Wei

Zhou

et al. 2021

Gels

View full text Add to dashboard Cite

show abstract

“…It was also concluded from SEM images that cells can adhere to such nanofibers even better than pure PCL cells. As a result, sufficient traction and mineralization through this BSA/PCL nanofiber biohybrid membrane can accelerate the wound healing process and be considered a suitable option for wound dressing applications [28].…”

Section: New Advances In Wound Dressing Of Bioactive Nanofibersmentioning

confidence: 99%

“…One of the most practical electrospinning methods in tissue engineering is skin regeneration [22][23][24][25][26][27]. Synthetic polymers are not inherently bioactive, and by combining with some active components, the bioactivity of synthetic polymers can be improved [28]. The electrospinning technique allows the production of a suitable nanofibrous membrane to deliver bioactive agents or pharmacological agents to the damaged site of skin to accelerate the skin regeneration process [14,29].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bioactive Agent-Loaded Electrospun Nanofiber Membranes for Accelerating Healing Process: A Review

et al. 2021

View full text Add to dashboard Cite

Despite the advances that have been achieved in developing wound dressings to date, wound healing still remains a challenge in the healthcare system. None of the wound dressings currently used clinically can mimic all the properties of normal and healthy skin. Electrospinning has gained remarkable attention in wound healing applications because of its excellent ability to form nanostructures similar to natural extracellular matrix (ECM). Electrospun dressing accelerates the wound healing process by transferring drugs or active agents to the wound site sooner. This review provides a concise overview of the recent developments in bioactive electrospun dressings, which are effective in treating acute and chronic wounds and can successfully heal the wound. We also discuss bioactive agents used to incorporate electrospun wound dressings to improve their therapeutic potential in wound healing. In addition, here we present commercial dressings loaded with bioactive agents with a comparison between their features and capabilities. Furthermore, we discuss challenges and promises and offer suggestions for future research on bioactive agent-loaded nanofiber membranes to guide future researchers in designing more effective dressing for wound healing and skin regeneration.

show abstract

“…PCL-based electrospun nanofibers are the most used nanofibrous mats as angiogenic biomaterials. This scaffold allowed for the infiltration of pro-angiogenic growth factors, including VEGF-D. PCL has the advantage of being able to be associated with other components, such as BSA, which improves cell adhesion to nanofibers [ 19 ], or VEGF, to improve angiogenesis. It can be used as a mechanical barrier, but it can also be functionalized with active molecules, such as growth factors, to stimulate the eruption or innervation of the tooth [ 11 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Eruption of Bioengineered Teeth: A New Approach Based on a Polycaprolactone Biomembrane

et al. 2021

View full text Add to dashboard Cite

Obtaining a functional tooth is the ultimate goal of tooth engineering. However, the implantation of bioengineered teeth in the jawbone of adult animals never allows for spontaneous eruption due mainly to ankylosis within the bone crypt. The objective of this study was to develop an innovative approach allowing eruption of implanted bioengineered teeth through the isolation of the germ from the bone crypt using a polycaprolactone membrane (PCL). The germs of the first lower molars were harvested on the 14th day of embryonic development, cultured in vitro, and then implanted in the recipient site drilled in the maxillary bone of adult mice. To prevent the ankylosis of the dental germ, a PCL membrane synthesized by electrospinning was placed between the germ and the bone. After 10 weeks of follow-up, microtomography, and histology of the implantation site were performed. In control mice where germs were directly placed in contact with the bone, a spontaneous eruption of bioengineered teeth was only observed in 3.3% of the cases versus 19.2% in the test group where PCL biomembrane was used as a barrier (p < 0.1). This preliminary study is the first to describe an innovative method allowing the eruption of bioengineered tooth implanted directly in the jawbone of mice. This new approach is a hope for the field of tooth regeneration, especially in children with oligodontia in whom titanium implants are not an optimal solution.

show abstract

Biomimetic biohybrid nanofibers containing bovine serum albumin as a bioactive moiety for wound dressing

Cited by 26 publications

References 85 publications

Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth

Electrospun Fibers Derived from Peptide Coupled Amphiphilic Copolymers for Dorsal Root Ganglion (DRG) Outgrowth

Bioactive Agent-Loaded Electrospun Nanofiber Membranes for Accelerating Healing Process: A Review

Eruption of Bioengineered Teeth: A New Approach Based on a Polycaprolactone Biomembrane

Contact Info

Product

Resources

About