Biomimetic Elastomeric Bioactive Siloxane‐Based Hybrid Nanofibrous Scaffolds with miRNA Activation: A Joint Physico‐Chemical‐Biological Strategy for Promoting Bone Regeneration

Abstract

Rapid and efficient disease‐induced or critical‐size bone regeneration remains a challenge in tissue engineering due to the lack of highly bioactive biomaterial scaffolds. Physical structures such as nanostructures, chemical components such as silicon elements, and biological factors such as genes have shown positive effects on bone regeneration. Herein, a bioactive photoluminescent elastomeric silicate‐based nanofibrous scaffold with sustained miRNA release is reported for promoting bone regeneration based on a joint physico‐chemical‐biological strategy. Bioactive nanofibrous scaffolds are fabricated by cospinning poly (ε‐caprolactone) (PCL), elastomeric poly (citrates‐siloxane) (PCS), and bioactive osteogenic miRNA nanocomplexes (denoted PPM nanofibrous scaffolds). The PPM scaffolds possess uniform nanostructures, significantly enhanced tensile stress (≈15 MPa) and modulus (≈32 MPa), improved hydrophilicity (30–60°), controlled biodegradation, and strong blue fluorescence. Bioactive miRNA complexes are efficiently loaded into the nanofibrous matrix and exhibit long‐term release for up to 70 h. The PPM scaffolds significantly promote the adhesion, proliferation, and osteoblast differentiation of bone marrow stem cells in vitro and enhanced rat cranial defect restoration (12 weeks) in vivo. This work reports an attractive joint physico‐chemical‐biological strategy for the design of novel cell/protein‐free bioactive scaffolds for synergistic tissue regeneration.

Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration

“…The excellent antioxidant ability and conductive activity of FPCP hydrogel could enhance myoblast proliferation and muscle regeneration by decreasing the oxidative stress and reactive oxygen species and enhancing angiogenesis which has important role in tissue regeneration [ 18 ]. The bioactive performance for FPCP in stimulating tissue formation was similar with previously reported bioactive biomaterials [ 56 , 57 ]. Moreover, FPCP showed negligible cytotoxicity in C2C12 cells because of the better biocompatibility of each component.…”

Bioactive antiinflammatory antibacterial hemostatic citrate-based dressing with macrophage polarization regulation for accelerating wound healing and hair follicle neogenesis

“…1 B). In the spectra of PCE, the peak at 1731 cm −1 was identified as the carbonyl group (-C O-) in the ester bonds (–COO–) and amido bond (–CONH–) [ 26 ]. The disappearance of the peak at 1720 cm −1 indicated that the carboxyl group (-COOH) of IBU was successfully reacted with the amino group (-NH 2 ) of PCE polymer.…”

Bifunctional Cx43 Mimic Peptide Grafted Hyaluronic Acid Hydrogels Inhibited Tumor Recurrence and Stimulated Wound Healing for Postsurgical Tumor Treatment

“…[ 28 ] However, these materials usually have much disadvantages, such as inhomogeneous property, particle aggregation, and mechanical instability. [ 7a,29 ] Meanwhile, many peptides have been reported to be used for tumor therapy with the advantages of small molecular weight, selectivity, non‐immunogenicity, low toxicity, easy penetration of tumor cells, and no resistance. [ 21a,30 ] In this study, JM2, as a Cx43 mimic peptide, showed abilities to inhibit tumor recurrence through regulating tumor cell proliferation by promoting tumor cell apoptosis and affecting tumor cell cycle.…”