Bioactive antiinflammatory antibacterial hemostatic citrate-based dressing with macrophage polarization regulation for accelerating wound healing and hair follicle neogenesis

Liu, Wenguang; Wang, Min; Cheng, Wei; Niu, Wen; Chen, Mi; Luo, Meng; Xie, Chenxi; Leng, Tongtong; Zhang, Long; Lei, Bo

doi:10.1016/j.bioactmat.2020.09.008

Cited by 83 publications

(50 citation statements)

References 33 publications

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“…7 b indicate the hematoxylin-eosin (H&E) staining of the experimental groups (PLLA membrane and 20% PNA membrane) and control group. At 7 d, abundant inflammatory cells were infiltrated into the upper layers of dermis in the wound site in the control group, possibly because severe inflammation occurred at the wound site due to the lack of external protection [ 9 , 10 ]. Compared with the control group, mice treated with PLLA membrane displayed a significant decrease in inflammatory cells at the wound site.…”

Section: Resultsmentioning

confidence: 99%

“…Wound healing is a complicated process generally consisting of four successive and overlapped steps: (1) hemostasis phase; (2) inflammatory phase; (3) proliferative phase; and (4) maturation and remodeling phase [ 9 , 10 ]. Among them, inflammatory phase is a kind of significant procedure, where immune cells secrete pro-inflammatory cytokines to induce inflammatory cells to produce a large amount of reactive oxygen species (ROS), which are the important metabolites of oxygen, including peroxides, superoxide, the hydroxyl radical and singlet oxygen [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Zhang

Qiu

et al. 2021

Bioactive Materials

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Facing the high incidence of skin diseases, it is urgent to develop functional materials with high bioactivity for wound healing, where reactive oxygen species (ROS) play an important role in the wound healing process mainly via adjustment of immune response and neovasculation. In this study, we developed a kind of bioabsorbable materials with ROS-mediation capacity for skin disease therapy. Firstly, redox-sensitive poly(N-isopropylacrylamide-acrylic acid) (PNA) nanogels were synthesized by radical emulsion polymerization method using a disulfide molecule as crosslinker. The resulting nanogels were then incorporated into the nanofibrous membrane of poly( l -lactic acid) (PLLA) via airbrushing approach to offer bioabsorbable membrane with redox-sensitive ROS-balance capacity. In vitro biological evaluation indicated that the PNA-contained bioabsorbable membrane improved cell adhesion and proliferation compared to the native PLLA membrane. In vivo study using mouse wound skin model demonstrated that PNA-doped nanofibrous membranes could promote the wound healing process, where the disulfide bonds in them were able to adjust the ROS level in the wound skin for mediation of redox potential to achieve higher wound healing efficacy.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Zhang

Qiu

et al. 2021

Bioactive Materials

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“…Recently, the biomedical polymers based on citric acid have been developed and attracted much attention, due to their good biocompatibility, biomimetic viscoelasticity and low cost [ 24 , 25 ]. Our group also reported a series of citric acid-based hybrid polymers and demonstrated their promising biomedical applications in bioimaging, drug delivery/obesity therapy and skin regeneration [ [26] , [27] , [28] , [29] , [30] ]. Recent studies exhibited that citric acid-based polymers could efficiently improve the angiogenesis which was the vital step during the tissue formation [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

“…PPy@PDA was used to achieve photothermal effect, antioxidant ability and electronic conductivity. CMC and PCE which could stimulate cell proliferation with low cytotoxicity in our previous report [ 29 ], were served as the crosslinked network. F127-Phe-CHO was served as the temperature-sensitive component, which formed the gel network by Schiff-base reaction between its aldehyde group and quinone group on PPy@PDA and amino group of CMC and PCE.…”

Section: Introductionmentioning

confidence: 99%

Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration

Zhou

Juan

Wang

et al. 2021

Bioactive Materials

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The completed skeletal muscle regeneration resulted from severe injury and muscle-related disease is still a challenge. Here, we developed an injectable muscle-adhesive antioxidant conductive bioactive photothermo-responsive nanomatrix for regulating the myogenic differentiation and promoting the skeletal muscle regeneration in vivo . The multifunctional nanomatrix was composed of polypyrrole@polydopamine (PPy@PDA, 342 ± 5.6 nm) nanoparticles-crosslinked Pluronic F-127 (F127)-polycitrate matrix (FPCP). The FPCP nanomatrix demonstrated inherent multifunctional properties including excellent photothermo-responsive and shear-thinning behavior, muscle-adhesive feature, injectable ability, electronic conductivity (0.48 ± 0.03 S/m) and antioxidant activity and photothermal function. The FPCP nanomatrix displayed better photothermal performance with near-infrared irradiation, which could provide the photo-controlled release of protein (91% ± 2.6% of BSA was released after irradiated 3 times). Additionally, FPCP nanomatrix could significantly enhance the cell proliferation and myogenic differentiation of mouse myoblast cells (C2C12) by promoting the expressions of myogenic genes (MyoD and MyoG) and myosin heavy chain (MHC) protein with negligible cytotoxicity. Based on the multifunctional properties, FPCP nanomatrix efficiently promoted the full-thickness skeletal muscle repair and regeneration in vivo , through stimulating the angiogenesis and myotube formation. This study firstly indicated the vital role of multifunctional PPy@PDA nanoparticles in regulating myogenic differentiation and skeletal muscle regeneration. This work also suggests that rational design of bioactive matrix with multifunctional feature would greatly enhance the development of regenerative medicine.

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“…interleukins, monocytes and neutrophils) [ 45 ]. Excessive/prolonged inflammation may inhibit wound healing and an anti-inflammatory dressing can accelerate wound healing [ 46 ]. In addition, re-epithelialization is an essential event during the wound healing process.…”

Section: Resultsmentioning

confidence: 99%

3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

Tang

et al. 2021

Regenerative Biomaterials

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Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns.

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Bioactive antiinflammatory antibacterial hemostatic citrate-based dressing with macrophage polarization regulation for accelerating wound healing and hair follicle neogenesis

Cited by 83 publications

References 33 publications

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Injectable muscle-adhesive antioxidant conductive photothermal bioactive nanomatrix for efficiently promoting full-thickness skeletal muscle regeneration

3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

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