Incorporating redox-sensitive nanogels into bioabsorbable nanofibrous membrane to acquire ROS-balance capacity for skin regeneration

Abstract

Facing the high incidence of skin diseases, it is urgent to develop functional materials with high bioactivity for wound healing, where reactive oxygen species (ROS) play an important role in the wound healing process mainly via adjustment of immune response and neovasculation. In this study, we developed a kind of bioabsorbable materials with ROS-mediation capacity for skin disease therapy. Firstly, redox-sensitive poly(N-isopropylacrylamide-acrylic acid) (PNA) nanogels were synthesized by radical emulsion polymerization method using a disulfide molecule as crosslinker. The resulting nanogels were then incorporated into the nanofibrous membrane of poly( l -lactic acid) (PLLA) via airbrushing approach to offer bioabsorbable membrane with redox-sensitive ROS-balance capacity. In vitro biological evaluation indicated that the PNA-contained bioabsorbable membrane improved cell adhesion and proliferation compared to the native PLLA membrane. In vivo study using mouse wound skin model demonstrated that PNA-doped nanofibrous membranes could promote the wound healing process, where the disulfide bonds in them were able to adjust the ROS level in the wound skin for mediation of redox potential to achieve higher wound healing efficacy.

Anti-oxidant anti-inflammatory and antibacterial tannin-crosslinked citrate-based mussel-inspired bioadhesives facilitate scarless wound healing

“…Infection and other pro-inflammatory factors left unchecked lead to chronic inflammatory reactions and significantly delayed wound healing [ [1] , [2] , [3] ]. During the inflammatory phase, immune cells secrete pro-inflammatory cytokines, inducing the production of reactive oxygen species (ROS), such as hydrogen peroxide (H 2 O 2 ), hydroxyl radical (OH·) and superoxide anion radical (O 2 ˙ − ), by resident inflammatory cells [ [4] , [5] , [6] ]. Excessive accumulation of ROS in infected wounds can overwhelm the native antioxidant defense system, strengthen the inflammatory reaction, and thus delay wound healing [ 7 , 8 ] and cause scar formation [ [9] , [10] , [11] , [12] ], resulting in skin dysfunction and adverse cosmetic appearance [ 13 ].…”

Biomaterials for Helicobacter pylori therapy: therapeutic potential and future perspectives

“…Polymeric nanoparticles are produced using polymeric materials (such as chitosan, ethylcellulose, and poly(lactic-co-glycolic acid)) and colloidal organic compounds in nanosizes. 91 , 92 Compared with lipid-based nanoparticles, polymeric nanoparticles exhibit higher loading capacities and mechanical stabilities. 93 Chitosan, the most widely studied natural cationic polysaccharide polymer, exhibits good adhesion and antibacterial properties.…”

Reactive Oxygen Species-Based Biomaterials for Regenerative Medicine and Tissue Engineering Applications

“…The in vivo experiments further demonstrated a reduction in the oxidative stress and anti-inflammatory response, whereas an enhancement in ECs proliferation and neo-angiogenesis, ultimately translating into enhanced diabetic wound healing. Li and co-workers developed redox-sensitive poly (N-isopropylacrylamide-acrylic acid) (PNA) nanogels-incorporated nanofibrous poly ( l -lactic acid) membranes ( Zhang S. et al, 2021 ). The disulfide-containing PNA nanogels can balance the ROS level to create favorable microenvironment for tissue repair.…”