Biomimetic growth of bone-like apatite via simulated body fluid on hydroxyethyl cellulose/polyvinyl alcohol electrospun nanofibers

Chahal, Sugandha; Fathima, Shahitha Jahir Hussain; Yusoff, Mashitah M.

doi:10.3233/bme-130871

Cited by 18 publications

(12 citation statements)

References 25 publications

(25 reference statements)

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“…The polar groups on the surface of the hydrolyzed PCL and of the immobilized C-ECM acted as nucleating agents for apatite (Figure b). The local supersaturation of calcium and phosphate ions available in the solution precipitated on the surface of the fibers and mineral growth followed. , The AP coating resulted in the formation of micron-sized crystals surrounding the fibers and formed occasional aggregates in a cauliflower-like arrangement (Figure b), consistent with previously published findings using this method. , This morphology contrasts with the relatively featureless surface topography of bare PCL fibers and the C-ECM coating.…”

Section: Resultssupporting

confidence: 89%

Spatial Presentation of Tissue-Specific Extracellular Matrix Components along Electrospun Scaffolds for Tissue Engineering the Bone–Ligament Interface

Olvera

Sathy

Kelly

2020

ACS Biomater. Sci. Eng.

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The bone–ligament interface transitions from a highly organized type I collagen rich matrix to a nonmineralized fibrocartilage region and finally to a mineralized fibrocartilage region that interfaces with the bone. Therefore, engineering the bone–ligament interface requires a biomaterial substrate capable of maintaining or directing the spatially defined differentiation of multiple cell phenotypes. To date the appropriate combination of biophysical and biochemical factors that can be used to engineer such a biomaterial substrate remain unknown. Here we show that microfiber scaffolds functionalized with tissue-specific extracellular matrix (ECM) components can direct the differentiation of MSCs toward the phenotypes seen at the bone–ligament interface. Ligament-ECM (L-ECM) promoted the expression of the ligament-marker gene tenomodulin (TNMD) and higher levels of type I and III collagen expression compared to functionalization with commercially available type I collagen. Functionalization of microfiber scaffolds with cartilage-ECM (C-ECM) promoted chondrogenesis of MSCs, as evidenced by adoption of a round cell morphology and increased SRY-box 9 (SOX9) expression in the absence of exogenous growth factors. Next, we fabricated a multiphasic scaffold by controlling the spatial presentation of L-ECM and C-ECM along the length of a single electrospun microfiber construct, with the distal region of the C-ECM coated fibers additionally functionalized with an apatite layer (using simulated body fluid) to promote endochondral ossification. These ECM functionalized scaffolds promoted spatially defined differentiation of MSCs, with higher expression of TNMD observed in the region functionalized with L-ECM, and higher expression of type X collagen and osteopontin (markers of endochondral ossification) observed at the end of the scaffold functionalized with C-ECM and the apatite coating. Our results demonstrate the utility of tissue-specific ECM derived components as a cue for directing MSC differentiation when engineering complex multiphasic interfaces such as the bone–ligament enthesis.

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Section: Resultssupporting

confidence: 89%

Spatial Presentation of Tissue-Specific Extracellular Matrix Components along Electrospun Scaffolds for Tissue Engineering the Bone–Ligament Interface

Olvera

Sathy

Kelly

2020

ACS Biomater. Sci. Eng.

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“…These scaffolds have a tensile strength >10 MPa and are biocompatible (Zhou et al, 2013; Zhang et al, 2015). The electrospun nanofibers with different weight ratios can be used to produce biomimetic bone structures (Chalal et al, 2014).…”

Section: Applicationsmentioning

confidence: 99%

“…Pore sizes ranging from 50 to 300 μm can be fabricated without affecting the surround material (Rodríguez et al, 2014). These constructs can be further processed on the nanoscale to become mineralized to an extent that resembles in vivo hydroxyapatite levels (Chalal et al, 2014; Rodríguez et al, 2014). The porous mineralized scaffolds increase osteoblast attachment and cell density at the pore sites (Rodríguez et al, 2014).…”

Section: Applicationsmentioning

confidence: 99%

Cellulose Biomaterials for Tissue Engineering

Hickey

Pelling

2019

Front. Bioeng. Biotechnol.

340

218

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In this review, we highlight the importance of nanostructure of cellulose-based biomaterials to allow cellular adhesion, the contribution of nanostructure to macroscale mechanical properties, and several key applications of these materials for fundamental scientific research and biomedical engineering. Different features on the nanoscale can have macroscale impacts on tissue function. Cellulose is a diverse material with tunable properties and is a promising platform for biomaterial development and tissue engineering. Cellulose-based biomaterials offer some important advantages over conventional synthetic materials. Here we provide an up-to-date summary of the status of the field of cellulose-based biomaterials in the context of bottom-up approaches for tissue engineering. We anticipate that cellulose-based material research will continue to expand because of the diversity and versatility of biochemical and biophysical characteristics highlighted in this review.

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“…Additionally, the osteogenic differentiation of the BMSCs proved the potential of the HA-deposited collagen-coated PLGA electrospun mesh in BTE. Onto the blend of electrospun nanofibers from hydroxyethylcellulose (HEC) and polyvinyl alcohol (PVA), concentrated SBF (10×) was also utilized to coat them with bone-like apatite within 2 days [ 152 ].…”

Section: Simulated Body Fluids For Electrospun-based Bone Scaffoldsmentioning

confidence: 99%

Bone Mineralization in Electrospun-Based Bone Tissue Engineering

Lim

2022

Polymers

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Increasing the demand for bone substitutes in the management of bone fractures, including osteoporotic fractures, makes bone tissue engineering (BTE) an ideal strategy for solving the constant shortage of bone grafts. Electrospun-based scaffolds have gained popularity in BTE because of their unique features, such as high porosity, a large surface-area-to-volume ratio, and their structural similarity to the native bone extracellular matrix (ECM). To imitate native bone mineralization through which bone minerals are deposited onto the bone matrix, a simple but robust post-treatment using a simulated body fluid (SBF) has been employed, thereby improving the osteogenic potential of these synthetic bone grafts. This study highlights recent electrospinning technologies that are helpful in creating more bone-like scaffolds, and addresses the progress of SBF development. Biomineralized electrospun bone scaffolds are also reviewed, based on the importance of bone mineralization in bone regeneration. This review summarizes the potential of SBF treatments for conferring the biphasic features of native bone ECM architectures onto electrospun-based bone scaffolds.

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Biomimetic growth of bone-like apatite via simulated body fluid on hydroxyethyl cellulose/polyvinyl alcohol electrospun nanofibers

Cited by 18 publications

References 25 publications

Spatial Presentation of Tissue-Specific Extracellular Matrix Components along Electrospun Scaffolds for Tissue Engineering the Bone–Ligament Interface

Spatial Presentation of Tissue-Specific Extracellular Matrix Components along Electrospun Scaffolds for Tissue Engineering the Bone–Ligament Interface

Cellulose Biomaterials for Tissue Engineering

Bone Mineralization in Electrospun-Based Bone Tissue Engineering

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