Biomimetic mineralization of nitrile hydratase into a mesoporous cobalt-based metal–organic framework for efficient biocatalysis

Pei, Xiaolin; Wu, Yifeng; Wang, Jiapao; Chen, Zhiji; Li, Wen; Su, Weike; Liu, Fangming

doi:10.1039/c9nr06470b

Cited by 54 publications

(25 citation statements)

References 41 publications

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“…Generally, the preparation of enzymes immobilized in MOFs is based on three strategies: encapsulation, surface immobilization, and pore trapping with presynthesized MOFs [180]. Enzymes encapsulated in porous materials such as porous nanoparticles or reversible micelles have better stability under adverse conditions such as high temperature, organic solvents, or extreme pH [181][182][183]. Figure 3 presents enzyme encapsulation in MOFs.…”

Section: Metal-organic Framework (Mofs)mentioning

confidence: 99%

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Cavalcante

Sousa

et al. 2021

Catalysts

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The market for industrial enzymes has witnessed constant growth, which is currently around 7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes naturally responsible for triglyceride hydrolysis. They are the most expansively used industrial biocatalysts, with wide application in a broad range of industries. However, these biocatalytic processes are usually limited by the low stability of the enzyme, the half-life time, and the processes required to solve these problems are complex and lack application feasibility at the industrial scale. Emerging technologies create new materials for enzyme carriers and sophisticate the well-known immobilization principles to produce more robust, eco-friendlier, and cheaper biocatalysts. Therefore, this review discusses the trending studies and industrial applications of the materials and protocols for lipase immobilization, analyzing their advantages and disadvantages. Finally, it summarizes the current challenges and potential alternatives for lipases at the industrial level.

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Section: Metal-organic Framework (Mofs)mentioning

confidence: 99%

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Cavalcante

Sousa

et al. 2021

Catalysts

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“…Other than stability improvement, some research groups recently reported the biomimetic mineralization technique was also conducive to recycling performance and catalytic efficiency, bringing this technique one step closer to real applications in industry catalysis. [ 74–76 ] However, the inherited small pores of most MOFs may impede the diffusion of HRP substrates and could compromise the catalytic activity of HRP. To solve this problem, recently, Ge and co‐workers reported a method to encapsulate HRP in MOFs with engineered structural defects to enhance catalytic activity through microfluidic gradient mixing synthesis (Figure 3b).…”

Section: Mofs With Biocatalystsmentioning

confidence: 99%

Metal–Organic Frameworks as a Versatile Materials Platform for Unlocking New Potentials in Biocatalysis

Liu

Liang

Xue

et al. 2021

Small

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Biocatalysts immobilization with nanomaterials has promoted the development of biocatalysis significantly and made it an indispensable part of catalysis industries nowadays. Metal–organic frameworks (MOFs), constructed from organic linkers and metal ions or clusters, have raised significant interests for biocatalysts immobilization in recent years. The diversity of building units, molecular‐scale tunability, and modular synthetic routes of MOFs greatly expand its ability as the host to integrate with biocatalysts. In this review, the general synthetic strategies of MOFs with biocatalysts are first summarized. Then, the recent progress of MOFs as a versatile host for a series of biocatalysts, including natural enzymes, nanozymes, and organism‐based biocatalysts, followed by the introduction of MOFs themselves as biocatalysts, is discussed. Furthermore, the stimuli‐responsive properties of MOFs themselves or the additional functionalization of protein, polymer, and peptide within/on MOF that enable the biocatalysts with the controllable and tunable behavior are also summarized, which could unlock new potentials in biocatalysis. Finally, a perspective of the upcoming challenges, potential impacts, and future directions of biocatalytic MOFs is provided.

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“…Besides, biological metal-organic frameworks (BioMOFs) were recently found to be ideal candidates for NHase immobilization (Wang et al, 2020). Pei et al (2020) encapsulated a NHase from A. manganoxydans into the cobalt-based mesoporous MOFs. The biomimetic mineralized NHase showed high catalytic activity toward 3cyanopyridine and remained 40% of the maximum activity at 70 • C (Pei et al, 2020).…”

Section: Applications Of Nhasementioning

confidence: 99%

“…Pei et al (2020) encapsulated a NHase from A. manganoxydans into the cobalt-based mesoporous MOFs. The biomimetic mineralized NHase showed high catalytic activity toward 3cyanopyridine and remained 40% of the maximum activity at 70 • C (Pei et al, 2020). All of these advances in NHase immobilization might gain popularity in industrial settings in the near future.…”

Section: Applications Of Nhasementioning

confidence: 99%

Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications

Cheng

Xia

Zhou

2020

Front. Bioeng. Biotechnol.

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Nitrile hydratase (NHase, EC 4.2.1.84) is one type of metalloenzyme participating in the biotransformation of nitriles into amides. Given its catalytic specificity in amide production and eco-friendliness, NHase has overwhelmed its chemical counterpart during the past few decades. However, unclear catalytic mechanism, low thermostablity, and narrow substrate specificity limit the further application of NHase. During the past few years, numerous studies on the theoretical and industrial aspects of NHase have advanced the development of this green catalyst. This review critically focuses on NHase research from recent years, including the natural distribution, gene types, posttranslational modifications, expression, proposed catalytic mechanism, biochemical properties, and potential applications of NHase. The developments of NHase described here are not only useful for further application of NHase, but also beneficial for the development of the fields of biocatalysis and biotransformation.

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Biomimetic mineralization of nitrile hydratase into a mesoporous cobalt-based metal–organic framework for efficient biocatalysis

Abstract: Recombinant cobalt-type NHase was encapsulated into ZIF-67 to improve its thermal stability and catalytic efficiency by a biomimetic mineralization strategy.

Cited by 54 publications

References 41 publications

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Current Status and Future Perspectives of Supports and Protocols for Enzyme Immobilization

Metal–Organic Frameworks as a Versatile Materials Platform for Unlocking New Potentials in Biocatalysis

Recent Advances and Promises in Nitrile Hydratase: From Mechanism to Industrial Applications

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