Biomimetic Mineralizing Agents Recover the Micro Tensile Bond Strength of Demineralized Dentin

Abstract

Biomimetic remineralization is an approach that mimics natural biomineralization, and improves adhesive procedures. The aim of this paper was to investigate the influence of Dentin Caries-like Lesions (DCLL)-Producing Model on microtensile bond strength (μTBS) of etch and rinse adhesive systems and investigate the effect of remineralizing agents such as Sodium Fluoride (NaF), MI Paste™ (MP) and Curodont™ Repair (CR) on caries-affected dentin (n = 6). Nine groups were established: (1) Sound dentin; (2) Demineralized dentin/Chemical DCLL: (3) Demineralized dentin/Biological DCLL; (4) Chemical/DCLL + NaF; (5) Chemical/DCLL + MP; (6) Chemical/DCLL + CR; (7) Biological/DCLL + NaF; (8) Biological/DCLL + MP; (9) Biological/DCLL + CR. Then all dentin blocks were subjected to a bonding procedure with Adper™ Single Bond 2 adhesive system/Filtek Z350XT 4 mm high block, following this they were immersed in deionized water/24 h and then sectioned with ≅1 mm2 beams. The μTBS test was conducted at 1 mm/min/500 N loading. Failure sites were evaluated by SEM (scanning electron microscopy (150×). μTBS data were submitted to factorial ANOVA and Tukey’s test (p < 0.05). The highest values were found when demineralized dentin was treated with MP and CR, regardless caries lesion depth (p < 0.05). There was a predominance of adhesive/mixed in the present study. It was concluded that the use of the artificial dentin caries production models produces differences in the μTBS. Additionally MP and CR remineralizing agents could enhance adhesive procedures even at different models of caries lesion.

Microtensile bond strength of TTEMA/TEGDMA remineralizing adhesive to early carious enamel lesions

“…The incorporation of silanized particles into resin adhesive did not result in deterioration of bond strength to enamel which is in agreement with other studies 29,[31][32][33][34] . Unfortunately, no results were reported about the effect of mixing nHA with resin adhesives on bond strength to ECE.…”

Non-Collagenous Dentin Protein Binding Sites Control Mineral Formation during the Biomineralisation Process in Radicular Dentin

“…This potential requirement for active NCPs, therefore, highlights a clinical challenge. Nevertheless, it may be possible to use biomimetic analogues of phosphoproteins to re-establish the biological characteristics of the ECM [42]. However, the mechanisms underlying the function of biomimetic analogues and their actual ensembles in the dentin matrix remain a matter of debate, since the analogues cannot be proven to bind to the specific location, emulating the complex structure of the dentin layer.…”

Effect of polyvinylphosphonic acid on resin-dentin bonds and the cytotoxicity of mouse dental papilla cell-23

“…Biomimetic remineralization, an approach that mimics natural biomineralization, has been reported to play a crucial role in inducing amorphous calcium phosphate nanoparticles to remineralize type I collagen and improve adhesive procedures. 7,8 The evidence suggests that the application of the protease inhibitors might be less effective in promoting dentin remineralization. 23 In a previous study, the use of biomimetic remineralized materials provided durable resin-dentin bonds.…”

“…4,5 Biomimetic dentin remineralization, a valuable tool for maintaining the mechanical properties and biostability of collagen fibrils, is an emerging biomedical technology for improving the durability of the bond strength. [6][7][8] Remineralization of demineralized dentin may reduce collagen degradation, [9][10][11] and specific agents have been used for this purpose.…”