Biomimetic one-pot synthesis of gold nanoclusters/nanoparticles for targeted tumor cellular dual-modality imaging

Abstract

Biomimetic synthesis has become a promising green pathway to prepare nanomaterials. In this study, bovine serum albumin (BSA)-conjugated gold nanoclusters/nanoparticles were successfully synthesized in water at room temperature by a protein-directed, solution-phase, green synthetic method. The synthesized BSA-Au nanocomplexes have fluorescence emission (588 nm) of gold nanoclusters and surface plasmon resonance of gold nanoparticles. The BSA-Au nanocomplexes display non-cytotoxicity and excellent biocompatibility on MGC803 gastric cancer cells. After conjugation of folic acid molecules, the obtained BSA-Au nanocomplexes showed highly selective targeting for MGC803 cells and dual-modality dark-field and fluorescence imaging.

pH-sensitive Au–BSA–DOX–FA nanocomposites for combined CT imaging and targeted drug delivery

“…These findings suggested that Au-BSA-DOX-FA nanocomposites also simultaneously offer targeting therapy with less histotoxicity, owing to FA-mediated endocytosis and the intracellular release of DOX as a consequence. 47 …”

Folate-conjugated nanoparticles as a potent therapeutic approach in targeted cancer therapy

“…[53] regarding the toxicity of gold NPs both in vitro and in vivo [105]. Gold NPs have successfully been used for several biomedical approaches such as photo-thermal therapy [106,107], cancer diagnosis [108], tumor imaging [109,110], and drug delivery [111,108]. The plasmon resonance features of gold NPs enable their detection in biological systems [112].…”

“…Another group used BSA-conjugated gold NPs, which was surface modified by FA. They showed that while the BSAgold NPs had no effects on the MGC803 gastric cancer cells, FA-modified NPs could selectively target them [110]. Similarly, FA-conjugated 6-mercaptopurine (6MP)-loaded gold NPs could potently kill FR-expressing HeLa and KB cells both in vitro and in vivo [119].…”

“…Gold nanoparticles FA-berberine hydrochloride-gold NPs Berberine hydrochloride Berberine hydrochloride loaded gold NPs selectively kill FRexpressing cancer cells [118] FA-BSA-gold NPs -BSA-conjugated gold NPs selectively target FR-expressing cancer cells [110] FA-6-mercaptopurine (6MP)-gold NPs 6-mercaptopurine (6MP) 6-mercaptopurine (6MP)-loaded gold NPs potently kill FR-expressing cancer cells both in vitro and in vivo.…”

Cellulase assisted synthesis of nano-silver and gold: Application as immobilization matrix for biocatalysis

“…CD spectra show that there is loss of secondary structure of enzyme during synthesis of silver NPs[50]. The secondary structural analysis reveals that native cellulase contains 85.61% of α-helix which is completely lost during the synthesis of silver NPs and there is formation of β-sheet (44.79%).…”