Biomimetic Remineralization of Resin-bonded Acid-etched dentin

Abstract

Degradation of denuded collagen within adhesive resin-infiltrated dentin is a pertinent problem in dentin bonding. A biomimetic remineralization scheme that incorporates non-classic crystallization pathways of fluidic amorphous nanoprecursors and mesoscopic transformation has been successful in remineralizing resin-free, acid-etched dentin, with evidence of intrafibrillar and interfibrillar remineralization. This study tested the hypothesis that biomimetic remineralization provides a means for remineralizing incompletely infiltrated resin-dentin interfaces created by etch-and-rinse adhesives. The remineralization medium consists of a Portland cement/simulated body fluid that includes polyacrylic acid and polyvinylphosphonic acid biomimetic analogs for amorphous calcium phosphate dimension regulation and collagen targeting. Both interfibrillar and intrafibrillar apatites became readily discernible within the hybrid layers after 2–4 months. In addition, intra-resin apatite clusters were deposited within the porosities of the adhesive resin matrices. The biomimetic remineralization scheme provides a proof-of-concept for the adoption of nanotechnology as an alternative strategy to extend the longevity of resin-dentin bonds.

Dentine bonding agents comprising calcium-silicates to support proactive dental care: Origins, development and future

“…While each experimental strategy that attempted to overcome these problems has its own benefits and reciprocal limitations 4) , progressive water replacement by hydroxyapatite generated during dentine remineralization may be a suitable strategy for extending the service life of resin-based dentine bonding procedures and its actualization has been a source of conjecture until now 5) . In this case, nano-leakage might only be a temporary phenomenon that could be solved by new hard-tissue formation 6) . Water may provide the aqueous environment for the infiltration of calcium phosphate (Ca/P) precursors into the gap zones of collagen fibrils to initiate nucleation and growth of newly formed mineral crystals.…”

Adhesive Materials with Bioprotective/Biopromoting Functions

“…Different from conventional remineralization, biomimetic remineralization represents a different approach to improve bonding stability by attempting to backfill the demineralized dentin collagen with liquid-like amorphous calcium phosphate (ACP) nanoprecursor particles that are stabilized by biomimetic analogs of non-collagenous proteins [67][68][69]. This strategy reproduced both intrafibrillar and interfibrillar minerals and recapitulated the dimension and order of the apatite crystallites that are found in natural dentin [70][71][72][73].…”

In vitro remineralization of hybrid layers using biomimetic analogs

“…Most biomimetic remineralization studies have used transmission electron microscopy to evaluate the interface between adhesive and resin and the degree of remineralization (Kinney et al, 2003;Carrilho et al, 2005;Trebacz and Wojtowicz, 2005;Tay and Pashley, 2009;Gu et al, 2010;Kim Y.K. et al, 2010b).…”