Biomimetic synthesis of functional silver nanoparticles using hairy roots of Panax ginseng for wheat pathogenic fungi treatment

Yugay, Yulia A.; Rusapetova, Tatiana; Mashtalyar, D. V.; Grigorchuk, Valeria P.; Vasyutkina, Elena; Kudinova, Olesya D.; Зенкина, К. В.; Trifuntova, I. B.; Карабцов, А. А.; Иванов, В. В.; Асеева, Т. А.; Bulgakov, Victor P.; Shkryl, Yuri N.

doi:10.1016/j.colsurfb.2021.112031

Cited by 23 publications

(8 citation statements)

References 40 publications

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“…The production of AgNPs, which makes up almost half of all manufactured nanomaterials globally, is over 450 tons [ 22 ]. The commercialization of AgNPs occurs most frequently in the fields of microelectronics, textiles, food and beverage packaging, and healthcare [ 23 ]. The traditional methods of chemical synthesis are expensive and result in environmentally hazardous and poisonous chemicals.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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Currently, nanoparticles and nanomaterials are widely used for biomedical applications. In the present study, silver nanoparticles (AgNPs) were successfully biosynthesized using a cell-free extract (CFE) of Bacillus thuringiensis MAE 6 through a green and ecofriendly method. The size of the biosynthesized AgNPs was 32.7 nm, and their crystalline nature was confirmed by XRD, according to characterization results. A surface plasmon resonance spectrum of AgNPs was obtained at 420 nm. Nanoparticles were further characterized using DLS and FTIR analyses, which provided information on their size, stability, and functional groups. AgNPs revealed less cytotoxicity against normal Vero cell line [IC50 = 155 μg/mL]. Moreover, the biosynthesized AgNPs exhibited promising antifungal activity against four most common Aspergillus, including Aspergillus niger, A. terreus, A. flavus, and A. fumigatus at concentrations of 500 μg/mL where inhibition zones were 16, 20, 26, and 19 mm, respectively. In addition, MICs of AgNPs against A. niger, A. terreus, A. flavus, and A. fumigatus were 125, 62.5, 15.62, and 62.5 μg/mL, respectively. Furthermore, the ultrastructural study confirmed the antifungal effect of AgNPs, where the cell wall’s integrity and homogeneity were lost; the cell membrane had separated from the cell wall and had intruded into the cytoplasm. In conclusion, the biosynthesized AgNPs using a CFE of B. thuringiensis can be used as a promising antifungal agent against Aspergillus species causing Aspergillosis.

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Section: Introductionmentioning

confidence: 99%

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Hashem

Saied

Amin

et al. 2022

JFB

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“…The first report describing how Carica papaya callus can be used for the synthesis of silver nanoparticles (AgNPs) in the range of 60-80 nm was published by Mude and coauthors [57]. Subsequently, metal nanoparticles were obtained using extracts of Taxus yunnanensis [58], Hyptis suaveolens [59], Citrullus colocynthis [60], Panax ginseng [61], Sesuvium portulacastrum [62], Linum usitatissimum [63], Nicotiana tabacum [64], and Solanum incanum [65] cell cultures. AgNPs and gold nanoparticles (AuNPs) have been produced using Michelia champaca calli [66].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Shkryl

Rusapetova

Yugay

et al. 2021

IJMS

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The present study reports a green chemistry approach for the rapid and easy biological synthesis of silver (Ag), gold (Au), and bimetallic Ag/Au nanoparticles using the callus extract of Lithospermum erythrorhizon as a reducing and capping agent. The biosynthesized nanoparticles were characterized with ultraviolet-visible (UV-Vis) spectroscopy, X-ray diffraction (XRD) analysis, and transmission electron microscopy (TEM). Our results showed the formation of crystalline metal nanostructures of both spherical and non-spherical shape. Energy dispersive X-ray (EDX) spectroscopy showed the characteristic peaks in the silver and gold regions, confirming the presence of the corresponding elements in the monometallic particles and both elements in the bimetallic particles. Fourier-transform infrared (FTIR) spectroscopy affirmed the role of polysaccharides and polyphenols of the L. erythrorhizon extract as the major reducing and capping agents for metal ions. In addition, our results showed that the polysaccharide sample and the fraction containing secondary metabolites isolated from L. erythrorhizon were both able to produce large amounts of metallic nanoparticles. The biosynthesized nanoparticles demonstrated cytotoxicity against mouse neuroblastoma and embryonic fibroblast cells, which was considerably higher for Ag nanoparticles and for bimetallic Ag/Au nanoparticles containing a higher molar ratio of silver. However, fibroblast migration was not significantly affected by any of the nanoparticles tested. The obtained results provide a new example of the safe biological production of metallic nanoparticles, but further study is required to uncover the mechanism of their toxicity so that the biomedical potency can be assessed.

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“…Yugay et al described a green chemistry technique for the biological production of Ag NPs utilizing extracts from Panax ginseng non-transformed callus, rolC transgenic callus, and hairy roots, as well as an assessment of their e ectiveness against crop-damaging fungal pathogens [30]. Fusarium graminearum, Fusarium avenaceum, Fusarium poae, and Fusarium sporotrichioides, which are involved with Fusarium head blight disease in cereals, were extremely a ected by the biosynthesized nanoparticles.…”

Section: Silver Nanoparticles (Ag Nps) Sekatawa Et Al Usedmentioning

confidence: 99%

“…Fusarium graminearum, Fusarium avenaceum, Fusarium poae, and Fusarium sporotrichioides, which are involved with Fusarium head blight disease in cereals, were extremely a ected by the biosynthesized nanoparticles. Furthermore, nanosilver's antifungal activity was e ectively applied to the surface sterilization of infected wheat kernels without affecting seed germination [30].…”

Section: Silver Nanoparticles (Ag Nps) Sekatawa Et Al Usedmentioning

confidence: 99%

Green Nanotechnology: Recent Research on Bioresource-Based Nanoparticle Synthesis and Applications

Alqarni

Alghamdi

Alshahrani

et al. 2022

Journal of Chemistry

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In the last decades, the idea of green nanotechnology has been expanding, and researchers are developing greener and more sustainable techniques for synthesizing nanoparticles (NPs). The major objectives are to fabricate NPs using simple, sustainable, and cost-effective procedures while avoiding the use of hazardous materials that are usually utilized as reducing or capping agents. Many biosources, including plants, bacteria, fungus, yeasts, and algae, have been used to fabricate NPs of various shapes and sizes. The authors of this study emphasized the most current studies for fabricating NPs from biosources and their applications in a wide range of fields. This review addressed studies that cover green techniques for synthesizing nanoparticles of Ag, Au, ZnO, CuO, Co3O4, Fe3O4, TiO2, NiO, Al2O3, Cr2O3, Sm2O3, CeO2, La2O3, and Y2O3. Also, their applications were taken under consideration and discussed.

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Biomimetic synthesis of functional silver nanoparticles using hairy roots of Panax ginseng for wheat pathogenic fungi treatment

Cited by 23 publications

References 40 publications

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Antifungal Activity of Biosynthesized Silver Nanoparticles (AgNPs) against Aspergilli Causing Aspergillosis: Ultrastructure Study

Biosynthesis and Cytotoxic Properties of Ag, Au, and Bimetallic Nanoparticles Synthesized Using Lithospermum erythrorhizon Callus Culture Extract

Green Nanotechnology: Recent Research on Bioresource-Based Nanoparticle Synthesis and Applications

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