Biomimetic synthesis of hydroxyapatite/bacterial cellulose nanocomposites for biomedical applications

Wan, Yizao; Huang, Yuan; Yuan, Caideng; Raman, Sudha R.; Zhu, Yong; Jiang, Haitao; He, Fang; Gao, Chuan

doi:10.1016/j.msec.2006.10.002

Cited by 307 publications

(179 citation statements)

References 51 publications

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“…Alternatively this could be obtained by chemical modification of BNC. Numerous chemical modifications of BNC have been reported in literature, aimed at promoting cell adhesion on BNC (Bodin et al, 2007a;Kalaskar et al, 2008), functionalizing it with drugs (Barud et al, 2008;Klechkovskaya et al, 2008;Nguyen et al, 2008) or tuning its biodegradability (Kusuhara et al, 2009) and producing nanocomposites (Grande et al, 2009;Wan et al, 2007). Interestingly Svensson et al (Svensson et al, 2005) demonstrated that chemical modifications such as sulfonation or phosphorylation could also increase BNC compressive modulus (i.e.…”

Section: Discussionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

202

109

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3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Secondly, the capacity of BNC to match these requirements is assessed. Finally, a biofabrication process to produce patient-specific BNC auricular implants is demonstrated.BNC samples (n = 78) with varying cellulose content (2.5 -15%) were compared using stressrelaxation indentation with human ear cartilage (n = 17, from 4 males, aged 49 -93 years old).Additionally, an auricle from a volunteer was scanned using a 3T MRI with a spoiled gradientecho sequence. A negative ear mold was produced from the MRI data in order to investigate if an ear-shaped BNC prototype could be produced from this mold.The results show that the instantaneous modulus E in , equilibrium modulus E eq , and maximum stress σ max of the BNC samples are correlated to effective cellulose content. Despite significantly different relaxation kinetics, the E in , E eq and σ max of BNC at 14% effective cellulose content reached values equivalent to ear cartilage (for E eq , BNC: 2.4 ± 0.4 MPa and ear cartilage: 3.3 ± 1.3 MPa). Additionally, this work shows that BNC can be fabricated into patient-specific auricular shapes. In conclusion, BNC has the capability to reach mechanical properties of relevance for ear cartilage replacement, and can be produced in patient-specific ear shapes.

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Section: Discussionmentioning

confidence: 99%

Mechanical evaluation of bacterial nanocellulose as an implant material for ear cartilage replacement

Nimeskern

Ávila

Sundberg

et al. 2013

Journal of the Mechanical Behavior of Biomedical Materials

202

109

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“…However, because bacterial cellulose is hydrogel-like, with a high water holding capacity, it may be possible to incorporate drugs and growth factors in the scaffold. In addition, the deposition of calcium phosphate mineral onto bacteria cellulose [31][32][33] may improve bone formation; model studies have shown that a hydroxyapatite layer increases the expression of mRNA encoding the bone matrix proteins osteocalcin, osteopontin, and bone sialoprotein [34,35].…”

Section: Cell Studymentioning

confidence: 99%

Microporous bacterial cellulose as a potential scaffold for bone regeneration

et al. 2010

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a b s t r a c tNanoporous cellulose biosynthesized by bacteria is an attractive biomaterial scaffold for tissue engineering due to its biocompatibility and good mechanical properties. However, for bone applications a microscopic pore structure is needed to facilitate osteoblast ingrowth and formation of a mineralized tissue. Therefore, in this study microporous bacterial cellulose (BC) scaffolds were prepared by incorporating 300-500 lm paraffin wax microspheres into the fermentation process. The paraffin wax microspheres were subsequently removed, and scanning electron microscopy confirmed a microporous surface of the scaffolds while Fourier transform infrared spectroscopy verified the elimination of paraffin and tensile measurements showed a Young's modulus of approximately 1.6 MPa. Microporous BC and nanoporous (control) BC scaffolds were seeded with MC3T3-E1 osteoprogenitor cells, and examined by confocal microscopy and histology for cell distribution and mineral deposition. Cells clustered within the pores of microporous BC, and formed denser mineral deposits than cells grown on control BC surfaces. This work shows that microporous BC is a promising biomaterial for bone tissue engineering applications.

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“…For this reason, with a few important exceptions, the structures obtained by soaking of various materials in supersaturated solutions containing ions of calcium and orthophosphate (e.g., Refs. [62][63][64][65][66][67]), those obtained by coating of various materials by calcium orthophosphates (e.g., Refs. [68][69][70][71][72][73]), as well as calcium orthophosphates coated by other compounds [74] have not been considered; however, composite coatings have been considered.…”

Section: General Information On Composites and Biocompositesmentioning

confidence: 99%