Biomineralization Induced by Cells of Sporosarcina pasteurii: Mechanisms, Applications and Challenges

Wu, Yang; Li, Huimin; Li, Yang

doi:10.3390/microorganisms9112396

Cited by 27 publications

(16 citation statements)

References 104 publications

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“…536 S. pasteurii, a Gram-positive bacterium, is well-known for its ability to promote MICP through its ureolysis activities. 453 The enzyme urease, which is secreted by S. pasteurii in high quantity, catalyzes the hydrolysis of urea and eventually leads to calcium carbonate precipitation in the extracellular space. This biochemical process glues the particles around the cells together to form a composite material, similar to cement's function in concrete.…”

Section: Structural Materialsmentioning

confidence: 99%

“…Bacterial spores remain dormant in concrete; once damage occurs, the infiltrating rainwater will activate these spores. The revived microorganisms can fill the spaces of the cracks through microbially induced calcium carbonate precipitation (MICP) . In another example, Heveran et al employed the photosynthetic Synechococcus sp.…”

Section: Engineering Living Materials From a Materials Science Perspe...mentioning

confidence: 99%

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Engineered Living Materials For Sustainability

Wang

Huang

et al. 2022

Chem. Rev.

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Recent advances in synthetic biology and materials science have given rise to a new form of materials, namely engineered living materials (ELMs), which are composed of living matter or cell communities embedded in self-regenerating matrices of their own or artificial scaffolds. Like natural materials such as bone, wood, and skin, ELMs, which possess the functional capabilities of living organisms, can grow, self-organize, and self-repair when needed. They also spontaneously perform programmed biological functions upon sensing external cues. Currently, ELMs show promise for green energy production, bioremediation, disease treatment, and fabricating advanced smart materials. This review first introduces the dynamic features of natural living systems and their potential for developing novel materials. We then summarize the recent research progress on living materials and emerging design strategies from both synthetic biology and materials science perspectives. Finally, we discuss the positive impacts of living materials on promoting sustainability and key future research directions.

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Section: Structural Materialsmentioning

confidence: 99%

Section: Engineering Living Materials From a Materials Science Perspe...mentioning

confidence: 99%

Engineered Living Materials For Sustainability

Wang

Huang

et al. 2022

Chem. Rev.

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“…The process is based on metabolic activity of the bacteria. The urea is hydrolyzed to ammonia and carbon dioxide by Sporosarcina pasteurii due to urease and the formation of ammonia leads to an increase in pH of the environment to induce calcite precipitation when calcium ions are present (Eryürük et al 2015a , b ; Wu et al 2021 ). To provide Ca ++ , the hydrolysis of urea in a solution with calcium chloride was used in the researches mostly (Harkes et al 2010 ; Okwadha and Li 2010 ; Hsu et al 2018 ; Saricicek et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Effect of cell density on decrease in hydraulic conductivity by microbial calcite precipitation

Eryürük

2022

AMB Expr

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The effect of number of cells deposited on decrease in hydraulic conductivity of porous media using CaCO3 precipitation induced by Sporosarcina pasteurii (ATCC 11,859) was examined in columns packed with glass beads in the range of 0.25 mm and 3 mm in diameter. After resting Sporosarcina pasteurii cells were introduced into the columns, a precipitation solution, which consisted of 500 mM CaCl2 and 500 mM urea, was introduced under continuous flow conditions. It was shown that hydraulic conductivity was decreased by formation of microbially induced CaCO3 precipitation from between 8.37 * 10−1 and 6.73 * 10−2 cm/s to between 3.69 * 10−1 and 1.01 * 10−2 cm/s. The lowest hydraulic conductivity was achieved in porous medium consisting of the smallest glass beads (0.25 mm in diameter) using the highest density of cell suspension (OD600 2.25). The number of the deposited cells differed depending on the glass bead size of the columns. According to the experiments, 7 * 10−9 g CaCO3 was produced by a single resting cell. The urease activity, which led CaCO3 precipitation, depended on presence of high number of cells deposited in the column because the nutrients were not included in the precipitation solution and consequently, the amount of CaCO3 precipitated was proportional with the cell number in the column. A mathematical model was also developed to investigate the experimental results, and statistical analysis was also performed.

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“…), and it can occur through following mechanisms, such as: urea hydrolysis, photosynthesis, sulfate reduction, denitrification, ammonification, and methane oxidation. Of these mechanisms, the metabolic pathways of ureolytic bacteria have been examined most extensively due to the simple functional principle in urea hydrolysis catalyzed by secreted urease [ 5 , 6 , 7 , 8 ]. The biomineralization through urease mechanism can be simplified as follows: bacterial cells which are negative charged adsorb calcium ions from the environmental solution (calcium chloride, calcium acetate, calcium lactate, and calcium gluconate); urea added is hydrolyzed by bacterial urease producing CO 3 −2 and NH 4 + ; CO 3 −2 combines with Ca 2+ to precipitate calcium carbonate crystals; finally, the bacterial cells are surrounded by carbonate crystals.…”

Section: Introductionmentioning

confidence: 99%

“…In last few years, there has been a growing interest in the use of microbially precipitated CaCO 3 as biomaterials, and more studies have been published [ 7 , 23 , 24 , 25 , 26 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Highlighting Bacteria with Calcifying Abilities Suitable to Improve Mortar Properties

Răut

Constantin

Petre³

et al. 2022

Materials

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Biomineralization, the use of microorganisms to produce calcium carbonate, became a green solution for application in construction materials to improve their strength and durability. The calcifying abilities of several bacteria were investigated by culturing on a medium with urea and calcium ions. The characterization of the precipitates from bacterial cultures was performed using X-ray diffraction, Fourier transform infrared spectroscopy, and thermogravimetric analysis. The formation of carbonate crystals was demonstrated by optical and scanning electron microscopy. Water absorption and compressive strength measurements were applied to mortars embedded with sporal suspension. The efficiency of the supplementation of mortar mixtures with bacterial cells was evaluated by properties, namely the compressive strength and the water absorption, which are in a relationship of direct dependence, the increase in compressive strength implying the decrease in water absorption. The results showed that Bacillus subtilis was the best-performing bacterium, its introduction into the mortar producing an increase in compressive strength by 11.81% and 9.50%, and a decrease in water absorption by 11.79% and 10.94%, after 28 and 56 days of curing, respectively, as compared to standards. The exploitation of B. subtilis as a calcifying agent can be an interesting prospect in construction materials.

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Biomineralization Induced by Cells of Sporosarcina pasteurii: Mechanisms, Applications and Challenges

Cited by 27 publications

References 104 publications

Engineered Living Materials For Sustainability

Engineered Living Materials For Sustainability

Effect of cell density on decrease in hydraulic conductivity by microbial calcite precipitation

Highlighting Bacteria with Calcifying Abilities Suitable to Improve Mortar Properties

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