Biomineralization of an organophosphorus pesticide, Monocrotophos, by soil bacteria

Bhadbhade, Bharati J.; Sarnaik, Seema; Kanekar, Pradnya Pralhad

doi:10.1046/j.1365-2672.2002.01680.x

Cited by 102 publications

(55 citation statements)

References 15 publications

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“…Monocrotophos is a systemic pesticide and its action is mainly on organs such as skin, eyes and central nervous system. The half-life of monocrotophos in soil was reported to be 40 to 60 days (Sha, 1999;Tomlin, 2000;Bhadbhade et al, 2002a). Continuous and excessive use of OP compounds has led to the contamination of several ecosystems in different parts of the world (Cisar and Snyder, 2000;Tse et al, 2004).…”

Section: Monocrotophosmentioning

confidence: 99%

Biodegradation of monocrotophos by bacteria isolated from soil

Srinivasulu¹,

Nilanjan²,

Chakravarthi³

et al. 2017

Afr. J. Biotechnol.

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Bacteria were isolated by enrichment culture technique from groundnut (Arachis hypogaea L.) soils and tested for their ability to degrade monocrotophos in mineral salts medium under aerobic conditions in the laboratory. Based on some of the morphological and 16S rRNA gene sequence analysis, the isolates were identified as Rhodococcus phenolicus strain MCP1 and Rhodococcus ruber strain MCP-2. The initial (0-day) recovery of monocrotophos in the culture medium was 94%; and by the end of 4 th day, about 21% of added monocrotophos was lost from the uninoculated medium. By the end of 1 st , 2 nd , 3 rd and 4 th day 13, 20, 24, 30% and 18, 33, 37, 45% of monocrotophos was degraded, by R. phenolicus strain MCP1 and R. ruber strain MCP-2 respectively, when the mineral salts medium was supplemented with monocrotophos as a C source. Simultaneously 12, 22, 26, 30% and 18, 26, 37, 40% of Nmethylacetoacetamide a metabolite of monocrotophos was recovered in the media inoculated with the R. phenolicus strain MCP1 and R. ruber strain MCP-2 respectively, during the same period. Decrease in the amount of monocrotophos with a concomitant increase in the level of N-methylacetoacetamide clearly indicates the degradation of parent compound.

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Section: Monocrotophosmentioning

confidence: 99%

Biodegradation of monocrotophos by bacteria isolated from soil

Srinivasulu¹,

Nilanjan²,

Chakravarthi³

et al. 2017

Afr. J. Biotechnol.

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“…Different enzymes such as phosphatase and esterase [23,24], phosphotriesterase [8], carboxylesterase [25] and organophosphate hydrolase (OPH) [26] have been reported to degrade MCP.…”

Section: Saj Biotechnology Issn: 2375-6713mentioning

confidence: 99%

“…During chemical degradation MCP gets converted into N-methylacetoacetamide, which can be either directly measured or indirectly by the disappearance of MCP from the solution. Metabolic reactions taking place during the metabolism of MCP by microbial cultures are N-demethylation, O-demethylation, hydroxylation of N-methyl groups and cleavage of the phosphate-crotanamide linkage, [23,28,29], with the formation of O-desmethyl MCP monomethyl phosphate, dimethyl phosphate, N-methyl acetoacetamide and N-methylbutyramide. Although the basic mechanism of MCP degradation by enzyme fraction is not exactly known but it can be presumed that firstly MCP is broken down into secondary metabolite and then it is completely mineralized.…”

Section: Saj Biotechnolmentioning

confidence: 99%

Comparative Analysis of Chemical, Fungal and Enzymatic Degradation of MCP

Jain¹,

Garg²

2014

Scholarena Journal of Biotechnology

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In general three different methods are used for the cleanup of pesticides. These include chemical, microbial and enzymatic methods. Therefore, the present study is an endeavor to compare the efficiency of all the three methods for the study of degradation of monocrotophos (MCP) (150 µgml -1 ). Chemical method involved the use of 0.1N NaOH, whereas for the biological as well as enzymatic method, molecularly characterized fungal strain Aspergillus niger JQ660373 was used. %age of MCP degradation was assessed in the form of residual MCP concentration and hence degradation kinetics was premeditated. The results were confirmed by using analytical tools, High Performance Thin Layer Chromatography (HPTLC) and Fourier Transfom Infrared (FTIR). Results of the study demonstrated that degradation of MCP followed first order kinetics with the calculated half-life of 0.79 days in enzymatic method as compared to 3.16 and 4.21 days for fungal and chemical method. HPTLC chromatograms clearly indicate significant decrease in the standard MCP peak (rf 0.19-0.21) with increasing incubation duration. Molecular insight of MCP degradation as studied, suggests hydrolytic cleavage of MCP resulting in the formation of inorganic phosphates (-PO 4 ) and amines. The overall efficiency of degradation was found to be Enzymatic > Fungal > Chemical. The term "pesticide" embraces an enormous diversity of products that are used in a number of different activities and are intended for preventing, destroying, repelling or mitigating pests. Among newly developed pesticides, organophosphorus pesticides (OPPs) are most commonly used as they degrade faster in the environment. MCP (Dimethyl (E)-1-methyl-2 methyl carbamoyl vinyl phosphate) is an organophosphate with broad-spectrum contact and systemic activity against spur throated locust, sorghum midge, western flower thrips, aphids, the green vegetable bug, mites, the stem borer, potato tuber moth which are common pests of sorghum, sunflowers, tomatoes, cotton, potato, soybean and tobacco [1,2].Despite increasing crop productivity MCP is highly toxic to human beings. Mechanism of toxicity of MCP involves irreversible acetyl cholinesterase inhibition [3].Owing to its highly toxic nature, degradation of MCP from the environment is very essential. Degradation of MCP occurs by biotic as well as abiotic factors of the natural environment. These factors influence the degradation of MCP and act in tandem and complement one another in the microenvironment [4].Traditional cleanup methods of pesticides include the use of chemicals. These chemicals pose hazards on the soil fertility and soil microbial flaura and fauna. Therefore, these methods were discarded and biological methods emerged as a new tool for the degradation of pesticides.

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“…As shown in Figure 3, the number of copies of plasmid encoding catabolic genes relative to each DNA copy increased during degradation of phenanthrene, suggesting the increase of plasmid numbers in each bacterial cell. The catabolic genes harbored by a plasmid can be transferred to other bacteria, even taxonomically divergent hosts in the environment, and function well (Bhadbhade et al 2002). This indicates that plasmid-mediated bioaugmentation might be a possible strategy, especially for systems not suitable for the survival of isolated bacteria.…”

Section: Phenanthrene Degradation and Cell Growthmentioning

confidence: 99%

Molecular Characterization of a Bacterial Consortium Enriched from an Oilfield that Degrades Phenanthrene

et al. 2006

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Characterization of functional and phylogenetic genes was carried out on a bacterial consortium, enriched from a water treatment system of an oilfield, that could use phenanthrene as the sole carbon source. The mixed culture degraded 130 mg phenanthrene l )1 in 16 days, which is significantly faster than previously reported pure cultures. The existence of catabolic genes (nahAc, C23O) in the mixed culture was quantitated by most probable number PCR. The plasmid encoding phenanthrene catabolic genes increased relative to the chromosome genes. Heterogeneous bacteria were present according to both PCR denaturing gradient gel electrophoresis and cloning methods, suggesting the possible existence of cooperation between different biochemical PAH-transforming pathways.

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Biomineralization of an organophosphorus pesticide, Monocrotophos, by soil bacteria

Cited by 102 publications

References 15 publications

Biodegradation of monocrotophos by bacteria isolated from soil

Biodegradation of monocrotophos by bacteria isolated from soil

Comparative Analysis of Chemical, Fungal and Enzymatic Degradation of MCP

Molecular Characterization of a Bacterial Consortium Enriched from an Oilfield that Degrades Phenanthrene

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