Biomolecular functionalization for enhanced cell–material interactions of poly(methyl methacrylate) surfaces

Abstract

The integration of implants or medical devices into the body tissues requires of good cell–material interactions. However, most polymeric materials used for these applications lack on biological cues, which enhanced mid- and long-term implant failure due to weak integration with the surrounding tissue. Commonly used strategies for tissue–material integration focus on functionalization of the material surface by means of natural proteins or short peptides. However, the use of these biomolecules involves major drawbacks such as immunogenic problems and oversimplification of the constructs. Here, designed elastin-like recombinamers (ELRs) are used to enhance poly(methyl methacrylate) surface properties and compared against the use of short peptides. In this study, cell response has been analysed for different functionalization conditions in the presence and absence of a competing protein, which interferes on surface–cell interaction by unspecific adsorption on the interface. The study has shown that ELRs can induce higher rates of cell attachment and stronger cell anchorages than short peptides, being a better choice for surface functionalization.

Novel potential scaffold for periodontal tissue engineering

“…For the surface preactivation, the OH-Matrix, Zn-Matrix and Ca-Matrix surfaces were reacted with a sodium carbonate buffer solution (333 mM; pH=12.5) for 2 h and gently washed with water. Thus, due to the partial hydrolysis of ester bonds, carboxyl groups were disposed on their surfaces [7].…”

“…New nanostructured polymer membranes based on polymethylmethacrylate are proposed. Polymethylmethacrylates (PMMA) are non-reabsorbable but biocompatible polymers, which have been widely used to clinically fix prostheses to bone or for vertebroplasty, intraocular lenses, orthopaedic bone cements, skull defects reparation or mandibular reconstruction between others, due to its excellent tissue compatibility [6,7]. Presented polymeric solution may be spun.…”

“…PMMA shows weak interactivity against tissue cells, and promotes the formation of fibrotic tissue [7]. To enhance tissue-biomaterial interaction, and therefore tissue integration, strategies such as modification of surface properties have been previously employed.…”

Synthesis and compatibility evaluation of versatile mesoporous silica nanoparticles with red blood cells: an overview

“…All the undesired substances, such as by-products, cell debris, solvents, and chemical leover, should be cleaned and appropriately removed to reduce unwanted effects. 63 4.1.1.1. Static models.…”

Adding Functions to Biomaterial Surfaces through Protein Incorporation

“…Laminins have also been used in surface coating in vitro, with applications ranging from functionalizing matrix-mimicking scaffolds via carbodiimide-based crosslinking [215] to isolating human skeletal muscle myoblasts with up to 98% purity by plating on laminin-coated plates to eliminate common fibroblast contaminants. [216] Other ECM-based protein molecules used to biofunctionalize materials include elastin-like recombinamers (ELRs), which were found to mediate higher rates of cell attachment and stronger cell anchorages to poly(methyl methacrylate) [217] and poly(lactic) acid [218] surfaces, as well as preventing non-specific protein adsorption in a possible anti-fouling role. Finally, a variety of engineered proteins that combine specific surface-binding motifs with a biological function have also been developed, such as polyester-binding protein PhaP fused to cell-binding motifs such as RGD and IKVAV to coat hydrophobic surfaces and mediate neural stem cell binding, [219] or a herpes simplex virus type 1 (HSV-1) envelope glycoprotein H-derived protein used to functionalize materials such as dendrimers to improve their cellular uptake.…”