Biomolecule Photoimmobilization: Application in Scanning Probe Microscopy

Luginbühl, Reto; Collioud, A.; Siegenthaler, H.; Sigrist, Hans

doi:10.1007/978-94-011-0049-6_58

Cited by 2 publications

(2 citation statements)

References 15 publications

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“…The height of the Fab‘-SH layer is 4.4 nm larger than the fluorinated alkanethiolate monolayer in the grids (∼1.5−2 nm 12 ). This value is consistent with the molecular dimensions of Fab‘-SH determined by others after accounting for image distortions that arise from the finite size and shape of the probe tip . This magnitude in the change in topography was observed in images of several samples and for different locations on each sample.…”

Section: Resultssupporting

confidence: 90%

Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold

O'Brien¹,

Jones²,

Porter³

et al. 2000

Anal. Chem.

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This paper describes the construction and characterization of miniaturized antigenic immunosurfaces composed of spontaneously adsorbed Fab′-SH fragments on gold. Rabbit Fab′-SH fragments contain a free sulfhydryl group that forms a thiolate bond with a gold substrate as detailed by X-ray photoelectron spectroscopy. This approach creates surfaces of higher epitope density, a factor critical to the early detection of disease, than surfaces composed of adsorbed whole molecule IgG on gold. The viability and specificity of antigenic Fab′-SH immunosurfaces is demonstrated using atomic force microscopy and confocal fluorescence microscopy, and possible explanations for the larger epitope density are discussed.

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Section: Resultssupporting

confidence: 90%

Immunosensing Platforms Using Spontaneously Adsorbed Antibody Fragments on Gold

O'Brien¹,

Jones²,

Porter³

et al. 2000

Anal. Chem.

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show abstract

“…In a parallel approach, Bhatia and co-workers exposed a glass substrate modified with a thiol-terminated silane to ultraviolet light to create surfaces that resisted protein adsorption because of the highly hydrated, anion-terminated groups formed from the photochemistry. , Ultraviolet light has also been used to remove non-thiol silanes from selective portions of a SiO 2 surface to create hydrophobic/hydrophilic surface patterns used to prepare two-dimensional antibody arrays . In addition, photoactive cross-linking reagents can be used to pattern the spatial composition of a surface. − This approach has been employed for the generation of protein/enzyme nanostructures using an ultraviolet laser-generated interference pattern for the activation of photobiotin . Although not extensively used at present, we expect a significant expansion in the use of this technology …”

Section: Photolithographymentioning

confidence: 99%