“…About 2 mg of cyt c was dissolved in 0.3 ml of 0.1 M phosphate buffer solution of pH 6.5. Dispersion of CNTs in protein solution by sonication has been reported recently [49,50]. In the present work, we find that following similar procedure helps to disperse SWNTs in phosphate buffer solution.…”
Section: Preparation Of Swnt Functionalized Cyt C Electrodes: (Cyt C/supporting
confidence: 84%
“…Proteins containing heme groups such as HRP, hemoglobin, myoglobin, cytochrome c could be used to catalyze the reduction of H 2 O 2 [14,50,[54][55][56]. We have characterized electrocatalytic activity of cyt c electrodes towards reduction of H 2 O 2 .…”
Section: Electrocatalysis Of F-cyt C Electrodes To the Reduction Of Hmentioning
“…About 2 mg of cyt c was dissolved in 0.3 ml of 0.1 M phosphate buffer solution of pH 6.5. Dispersion of CNTs in protein solution by sonication has been reported recently [49,50]. In the present work, we find that following similar procedure helps to disperse SWNTs in phosphate buffer solution.…”
Section: Preparation Of Swnt Functionalized Cyt C Electrodes: (Cyt C/supporting
confidence: 84%
“…Proteins containing heme groups such as HRP, hemoglobin, myoglobin, cytochrome c could be used to catalyze the reduction of H 2 O 2 [14,50,[54][55][56]. We have characterized electrocatalytic activity of cyt c electrodes towards reduction of H 2 O 2 .…”
Section: Electrocatalysis Of F-cyt C Electrodes To the Reduction Of Hmentioning
“…24 A popular strategy for the construction of CNTs-based biosensors is to immobilize CNTs onto electrode using polymer coatings. Among them, polypyrrole (PPy) [25][26][27][28] and polyethyleneimine (PEI) 29 30 are successfully used for the entrapment of several molecules in various biosensors configurations.…”
Advances in nanoscience have allowed scientists to incorporate new nanomaterials in biosensing platforms. Carbon nanotubes are nanomaterials that facilitate the charge transfer between the bioelement and the transducer. Electrochemical impedance spectroscopy is a useful technique for the modified surface characterization. In the present approach electrochemical impedance spectroscopy was used to characterize the electrodes modified with different types of carbon nanotubes (single and multi-wall) according to their morphology and electrochemical behavior. By using Nyquist and Bode diagrams it was possible to assign the appropriate circuit considering all possible contributors. The charge transfer resistances as well as the time constants were calculated for all five types of investigated carbon nanotubes.
“…Conducting polymers (CPs), due to their structure, provide versatile electronic properties (metallic to insulator), low ionization potential and high electron affinity, offering an easy to use method for enzyme immobilization. However, most of these properties depend on the synthesis procedure used as well as on the nature of the polymer itself [14]. …”
The development and optimization of new biosensors with horseradish peroxidase immobilized in carbon nanotubes-polyethyleneimine or polypyrrole nanocomposite film at the surface of two types of transducer is described. The amperometric detection of acetaminophen was carried out at −0.2 V versus Ag/AgCl using carbon based-screen printed electrodes (SPEs) and glassy carbon electrodes (GCEs) as transducers. The electroanalytical parameters of the biosensors are highly dependent on their configuration and on the dimensions of the carbon nanotubes. The best limit of detection obtained for acetaminophen was 1.36 ± 0.013 μM and the linear range 9.99–79.01 μM for the HRP-SWCNT/PEI in GCE configuration. The biosensors were successfully applied for the detection of acetaminophen in several drug formulations.
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