“…This approach requires the organisms' response to external stressors to be directly studied and quantified (Zwart, 1995). The measurements of an organisms' physiological, morphological and biomolecular responses, such as lethal concentration, survivorship (Bonnail, Macías and Osta, 2019), biomass, growth (Hybská et al, 2020), damage to DNA and other genetic markers (Vieira et al, 2019), phytotoxicity, cytotoxicity, genotoxicity, mutagenity (Cavusoglu et al, 2010;Bilal et al, 2016;Artico, Migita and Menezes, 2018;Olusola and Solomon, 2018) and locomotion responses (Salánki et al, 2003) have been utilised in previous research. Experiments may be conducted in bioassays / mesocosms designed on simplifications of the natural environment, to control for the complexities of real-world field conditions.…”