Biomonitoring with Micronuclei Test in Buccal Cells of Female Farmers and Children Exposed to Pesticides of Maneadero Agricultural Valley, Baja California, Mexico

Castañeda-Yslas, Idalia Jazmin; Arellano-García, María Evarista; Garcia-Zarate, M. A.; Ruíz-Ruíz, Balam; Zavala-Cerna, María Guadalupe; Torres-Bugarı́n, Olivia

doi:10.1155/2016/7934257

Cited by 28 publications

(12 citation statements)

References 27 publications

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“…Dividing the entire sample of participants in the study (exposed and unexposed children) into sub-groups (low, medium, and high OC concentrations), however, revealed a statistically-significant increase in TAC in the individuals with higher OC concentrations in their hair. The prevalences of MNi identified in both schools are important (58%), and the average value of the frequency of MNi in each child was 0.2%, close to the value that is considered the normal average of frequencies of micronuclei in children, which ranges from 0.1 to 3.6% 58,65 .…”

Section: Effects On Children's Healthsupporting

confidence: 52%

“…Similar studies of the effects of pesticides on the frequency of MNi in buccal cells provide only inconsistent results 57 . Significant differences in the frequency of MNi have been demonstrated, for example, in women agricultural laborers with respect to an unexposed group 58 , while workers on banana plantations showed an increase in the frequency of micronuclei when compared to unexposed controls 59 . A study carried out with greenhouse workers found significant differences in nuclear alterations (MNi, BN,PK, KR) compared to a group that was not exposed to pesticides 60 ; however, those studies did not conduct analyses of pesticide concentrations that might reveal a direct association, but only considered potential exposures based on a list of the pesticides utilized.…”

Section: Discussionmentioning

confidence: 99%

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Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides

Anguiano-Vega

Cazares-Ramirez

Osten

et al. 2020

Sci Rep

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This study identified and determined organochloride pesticide (OCs) concentrations in hair samples from children at two elementary schools: one exposed to fumigations in agricultural fields, the other unexposed. Three concentrations of OCs levels in the hair were compared (high, medium, low), and total nuclear abnormalities in buccal cells were determined: micronuclei (MNi), condensed chromatin, karyorrhexis, pyknosis, binucleate cells, karyolysis, lobed nuclei, and apoptosis. No significant differences were found for the presence of MNi between the schoolchildren from the exposed and unexposed schools, but the prevalence of OCs in both schools was over 50%, as well as the frequencies of MNi in the children were over 58%. Findings show a significant difference between the frequency of MNi in the total sample of schoolchildren (exposed school + unexposed school) in relation to the concentration of OCs detected in their hair. The children from exposed school that showed the higher concentrations of OCs in hair had higher levels of genotoxic damage in the buccal cells; compared against children with lower concentrations of OCs. The most frequent nuclear abnormalities in the exposed children were lobed nuclei (79.4%), binucleate cells (66.66%), apoptosis (65.07), and MNi (58.7%). We determined the prevalence ratio (PR) and prevalence odds ratio (POR) for the presence of MNi in buccal cells in relation to the OCs concentrations in the hair samples. Both ratios were high for MNi [PR 3.93, 95% confidence interval (CI) 1.97–7.84, p = 0.0003; and POR 7.97, 95% CI 2.62–24.28, p = 0.0003], indicating a 7.97 times greater risk that the exposed children will present > 0.2% of MNi when OCs concentrations exceed 0.447 μg/g. These indicators may be useful biomarkers of genotoxic damage in children exposed to persistent, highly-toxic compounds. Results suggest the potential risk to which those schoolchildren are exposed on a daily basis due to fumigations in nearby agricultural fields.

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Section: Effects On Children's Healthsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 99%

Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides

Anguiano-Vega

Cazares-Ramirez

Osten

et al. 2020

Sci Rep

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“…These studies have suggested that children have a high risk of exposure to pesticides through contact with environmental contaminants on the floor as well as through hand-to-mouth behavior with dust and soil. The latter represents a significant source of pesticide exposure (Simcox et al 1995, Castañeda-Yslas 2016. Furthermore, the results of the review by Deziel et al (2015) found five studies with evidence of cleaning practices that had a potential impact on the concentration of pesticides in the dust at home.…”

Section: Para-occupational Exposure: Women Living In Agricultural Fammentioning

confidence: 99%

Pesticide Exposure Index: Practices Among Women From an Agricultural Community in Southeast Mexico

Pérez-Herrera

Vera-Avilés

Castillo-Burguete

et al. 2018

Rev. Int. Contam. Ambie.

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The adverse effects of pesticides on public health have been well documented in different populations across the world. Families of agricultural workers, particularly their wives, face pesticide exposures through a number of complex patterns even when they do not directly engage in agricultural work. Nonetheless, these patterns of exposure among women in agricultural communities remain understudied. Unfortunately, in Mexico there are no studies examining these patterns yet. In consequence, the main goals of this study were to: 1) evaluate pesticide exposure in a rural community of Southeast Mexico, 2) examine the patterns of environmental and para-occupational pesticide exposure, and 3) document the para-occupational and environmental pesticide exposure among women who are not agricultural workers but have an agricultural family or are wives of agricultural workers. We conducted a cross-sectional study of 78 women who did not themselves participate in any agricultural activity, but lived in an agricultural community. Questionnaires and interviews were used to construct a Pesticide Exposure Index (PEI) estimating the degree of pesticide exposure among women from agricultural families and wives of agricultural workers. Through the PEI we showed that women living in agricultural communities using pesticides are inherently exposed to a certain level of pesticides. With the PEI we showed that women from agricultural families, especially wives of agricultural workers, have a long-term para-occupational pesticide exposure that should not be underestimated because they are not agricultural workers.

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“…[1][2][3][4][5][6][7][8][9] Therefore, DNA damage varies considerably from one population to another.…”

Section: Dna Damages In Mexican Nativesmentioning

confidence: 99%

Micronuclei and nuclear anomalies in Mexico’s indigenous population

et al. 2017

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Objective. To determine the number of micronuclei and nuclear anomalies in Mexico's indigenous population. Materials and methods. One hundred twenty indigenous individuals were evaluated, including thirty from the ethnicities Cora, Huichol, Tarahumara and Tepehuano. The number of micronuclei (MN) and any nuclear abnormality (NA) in oral mucosa cells, including cells with nuclear buds, binucleated cells, cells with karyolysis, karyorrhetic, condensed chromatin and pyknotic cells were determined for each participant. Results. Tepehuano and Tarahumaras showed the greatest damage to DNA. The Tepehuano group presented the highest number of MN and NA, this being a significant difference (p < 0.05) compared with the rest of the studied groups. This group also presented the highest herbicide exposure (46.7%). In relation to the smoking and drinking habits, these were more frequent in the Tarahumara group (33.3 and 50% respectively). Conclusion. The ethnic diversity, habits and customs may influence the DNA nuclear integrity in the Amerindian groups. https://doi.org/10.21149/8318Resumen Objetivo. Determinar el número de micronúcleos y anomalías nucleares en la población indígena de México. Material y métodos. Se evaluó a ciento veinte indígenas, incluyendo treinta individuos de las etnias cora, huichol, tarahumara y tepehuana. A cada participante se le determinó el número de micronúcleos (MN) y de alguna anomalía nuclear (AN) en células de mucosa bucal, incluyendo células con brotes nucleares, binucleadas, cariolisis, cariorrexis, cromatina condensada y picnóticas. Resultados. Los tepehuanos y tarahumaras mostraron el mayor daño al ADN. El grupo tepehuano presentó el mayor número de MN y AN, con una diferencia significativa (p < 0.05) en comparación con el resto de los grupos estudiados; este grupo presentó también la mayor exposición a herbicidas (46.7%). En relación con los hábitos de fumar y beber, se presentaron con mayor frecuencia en el grupo tarahumara (33.3 y 50%, respectivamente). Conclusión. La diversidad étnica, hábitos y costumbres pueden influir la integridad del ADN en los grupos amerindios.

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Biomonitoring with Micronuclei Test in Buccal Cells of Female Farmers and Children Exposed to Pesticides of Maneadero Agricultural Valley, Baja California, Mexico

Cited by 28 publications

References 27 publications

Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides

Risk of genotoxic damage in schoolchildren exposed to organochloride pesticides

Pesticide Exposure Index: Practices Among Women From an Agricultural Community in Southeast Mexico

Micronuclei and nuclear anomalies in Mexico’s indigenous population

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