Bionic cartilage acellular matrix microspheres as a scaffold for engineering cartilage

Liu, Jun; Wang, Xiuyu; Lu, Gonggong; Tang, James; Wang, Yonghui; Zhang, Boqing; Sun, Yong; Lin, Hai; Wang, Qiguang; Liang, Jie; Fan, Yujiang; Zhang, Xingdong

doi:10.1039/c8tb02999g

Cited by 13 publications

(5 citation statements)

References 57 publications

(60 reference statements)

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“…The cartilage particles were decellularized using the previously optimized decellularization procedure. 25 In brief, the cartilage particles were soaked in high and low osmotic pressure solution three times, and then alternately soaked in liquid nitrogen and 37 °C water bath for five rounds for 10 min each time. Subsequently, cell membranes were broken with 0.5% Triton X-100 solution, DNase I solution and DNA enzyme working solution were added to remove any remaining DNA.…”

Section: Materials and Mmethodsmentioning

confidence: 99%

“…After drying, the cartilage was ground in a cryogenic grinder to obtain cartilage particles with the size of less than 100 μm. The cartilage particles were decellularized using the previously optimized decellularization procedure . In brief, the cartilage particles were soaked in high and low osmotic pressure solution three times, and then alternately soaked in liquid nitrogen and 37 °C water bath for five rounds for 10 min each time.…”

Section: Materials and Mmethodsmentioning

confidence: 99%

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Childhood Cartilage ECM Enhances the Chondrogenesis of Endogenous Cells and Subchondral Bone Repair of the Unidirectional Collagen–dECM Scaffolds in Combination with Microfracture

Cao

Wang

Chen

et al. 2021

ACS Appl. Mater. Interfaces

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Despite the formation of mechanically inferior fibrocartilage, microfracture (MF) still remains the gold standard to repair the articular cartilage defects in clinical settings. To date, although many tissue-engineering scaffolds have been developed to enhance the MF outcome, the clinical outcomes remain inconsistent. Decellularized extracellular matrix (dECM) is among the most promising scaffold for cartilage repair due to its inheritance of the natural cartilage components. However, the impact of dECM from different developmental stages on cellular chondrogenesis and therapeutic effect remains elusive, as the development of native cartilage involves the distinct temporal dependency of the ECM components and various growth factors. Herein, we hypothesized that the immature cartilage dECM at various developmental stages was inherently different, and would consequently impact the chondrogenic potential BMSCs. In this study, we fabricated three different unidirectional collagen−dECM scaffolds sourced from neonatal, childhood, and adolescent rabbit cartilage tissues, and identified the age-dependent biological variations, including DNA, cartilage-specific proteins, and growth factors; along with the mechanical and degradation differences. Consequently, the different local cellular microenvironments provided by these scaffolds led to the distinctive cell morphology, circularity, proliferation, chondrogenic genes expression, and chondrogenesis of BMSCs in vitro, and the different gross morphology, cartilage-specific protein production, and subchondral bone repair when in combination with microfracture in vivo. Together, this work highlights the immature cartilage dECM at different developmental stages that would result in the diversified effects to BMSCs, and childhood cartilage would be considered the optimal dECM source for the further development of dECM-based tissue engineering scaffolds in articular cartilage repair.

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Section: Materials and Mmethodsmentioning

confidence: 99%

Section: Materials and Mmethodsmentioning

confidence: 99%

Childhood Cartilage ECM Enhances the Chondrogenesis of Endogenous Cells and Subchondral Bone Repair of the Unidirectional Collagen–dECM Scaffolds in Combination with Microfracture

Cao

Wang

Chen

et al. 2021

ACS Appl. Mater. Interfaces

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“…And the in vivo results demonstrated that these microspheres could compensate for the inductive lack of polymers and facilitate osteochondral differentiation. Furthermore, Liu et al prepared bionic cartilage ECM microspheres derived from MSCs and demonstrated their remarkable efficacy in promoting chondrogenesis without the need for any additional serum or induction components [57].…”

Section: Composite Microspheresmentioning

confidence: 99%

Progress in microsphere-based scaffolds in bone/cartilage tissue engineering

Pan,

Su,

Yao

2023

Biomed. Mater.

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Bone/cartilage repair and regeneration have been popular and difficult issues in medical research. Tissue engineering is rapidly evolving to provide new solutions to this problem, and the key point is to design the appropriate scaffold biomaterial. In recent years, microsphere-based scaffolds have been considered suitable scaffold materials for bone/cartilage injury repair because microporous structures can form more internal space for better cell proliferation and other cellular activities, and these composite scaffolds can provide physical/chemical signals for neotissue formation with higher efficiency. This paper reviews the research progress of microsphere-based scaffolds in bone/chondral tissue engineering, briefly introduces types of microspheres made from polymer, inorganic and composite materials, discusses the preparation methods of microspheres and the exploration of suitable microsphere pore size in bone and cartilage tissue engineering, and finally details the application of microsphere-based scaffolds in biomimetic scaffolds, cell proliferation and drug delivery systems.

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“…Cartilage tissue injury is one of the common but not self-healable diseases in clinical orthopedics. − Artificial cartilage scaffolds have excellent mechanical and biological performance toward mimetic tissue environments and greatly relieve the patients’ physical and psychological distress. − To date, approaches to transplantation of these scaffolds have been widely applied as ideal replacements for traditional therapies for cartilage tissue regeneration and repair. Material matrices are indispensable parts in this field and play critical roles in realizing these properties. ,− Typically, porous, water-rich hydrogels are able to mimic extracellular matrices and provide similar microenvironments for cell proliferation, nutrition delivery, and tissue generation. − The introduction of functional components in hydrogels will improve their mechanical property, biocompatibility, diagnosis, and therapy performances.…”

Section: Introductionmentioning

confidence: 99%

Customizable Low-Friction Tough Hydrogels for Potential Cartilage Tissue Engineering by a Rapid Orthogonal Photoreactive 3D-Printing Design

Cheng

Zhang

et al. 2023

ACS Appl. Mater. Interfaces

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Hydrogels have demonstrated wide applications in tissue engineering, but it is still challenging to develop strong, customizable, lowfriction artificial scaffolds. Here, we report a rapid orthogonal photoreactive 3Dprinting (ROP3P) strategy to achieve the design of high-performance hydrogels in tens of minutes. The orthogonal ruthenium chemistry enables the formation of multinetworks in hydrogels via phenol-coupling reaction and traditional radical polymerization. Further Ca 2+ -cross-linking treatment greatly improves their mechanical properties (6.4 MPa at a critical strain of 300%) and toughness (10.85 MJ m −3 ). The tribological investigation reveals that the high elastic moduli of the as-prepared hydrogels improve their lubrication (∼0.02) and wear-resistance performances. These hydrogels are biocompatible and nontoxic and promote bone marrow mesenchymal stem cell adhesion and propagation. The introduction of 1-hydroxy-3-(acryloylamino)-1,1-propanediylbisphosphonic acid units can greatly enhance their antibacterial property to kill typical Escherichia coli and Staphylococcus aureus. Moreover, the rapid ROP3P can achieve hydrogel preparation in several seconds and is readily compatible with making artificial meniscus scaffolds. The printed meniscus-like materials are mechanically stable and can maintain their shape under long-term gliding tests. It is anticipated that these high-performance customizable low-friction tough hydrogels and the highly efficient ROP3P strategy could promote further development and practical applications of hydrogels in biomimetic tissue engineering, materials chemistry, bioelectronics, and so on.

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Bionic cartilage acellular matrix microspheres as a scaffold for engineering cartilage

Abstract: Bionic cartilage acellular matrix microspheres (BCAMMs) made from decelluarized bionic cartilage microspheres (BCMs).

Cited by 13 publications

References 57 publications

Childhood Cartilage ECM Enhances the Chondrogenesis of Endogenous Cells and Subchondral Bone Repair of the Unidirectional Collagen–dECM Scaffolds in Combination with Microfracture

Childhood Cartilage ECM Enhances the Chondrogenesis of Endogenous Cells and Subchondral Bone Repair of the Unidirectional Collagen–dECM Scaffolds in Combination with Microfracture

Progress in microsphere-based scaffolds in bone/cartilage tissue engineering

Customizable Low-Friction Tough Hydrogels for Potential Cartilage Tissue Engineering by a Rapid Orthogonal Photoreactive 3D-Printing Design

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