Bioorthogonal DNA Adsorption on Polydopamine Nanoparticles Mediated by Metal Coordination for Highly Robust Sensing in Serum and Living Cells

Meng, Yingcai; Liu, Peng; Zhou, Wenhu; Ding, Jinsong; Liu, Juewen

doi:10.1021/acsnano.8b03019

Cited by 123 publications

(97 citation statements)

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“…Under alkaline conditions, the PDA was synthesized by deprotonation and intermolecular Michael addition reaction with the oxidative self‐polymerized form of dopamine (DA) at the room temperature . Up to date, several PDA nanoparticles (NPs) have been reported and applied as sensing, biological studies, and cell imaging . However, it is rare to use DA or its derivatives to synthesize green fluorescent NPs for silver detection.…”

Section: Introductionmentioning

confidence: 95%

Biocompatible Nanoplatform Based on Mussel Adhesive Chemistry: Effective Assembly, Dual Mode Sensing, and Cellular Imaging Performance

Zhang

et al. 2019

Adv Materials Inter

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able consequences, such as organ failure, cytotoxicity, and brain damage. [6][7][8] Therefore, designing and developing a novel method to detect the concentrations of silver ions will be very significant in environmental and biological fields. To date, numerous analysis ways of monitoring Ag + ions in aqueous media were developed and they could be generalized as follows: such as atomic absorption spectrometry, inductively coupled plasma-mass spectrometry, high-performance liquid chromatography, UV-vis spectrometry, and fluorescence spectrometry. [9][10][11][12][13] Compared to the conventional methods with single signal transduction, the determination of Ag + ions with multiple responses could provide more convincing results. In this contribution, the nanoprobe can specifically detect Ag + ions not only by fluorescence via color changes but also it employs UV-vis absorption spectroscopy to recognize silver ions rapidly. The dual mode sensing is certainly superior to traditional fluorescence and the signals are collected in a more reliable way with accuracy and convenience.It has been well documented that the polydopamine (PDA) and its derivative materials have been extensively discussed in energy, catalytic, environmental, and biomedical fields. [14,15] Under alkaline conditions, the PDA was synthesized by deprotonation and intermolecular Michael addition reaction with the The condensation reaction between methyldopa sesquihydrate and ethylenediamine is reported and the assembled nanoplatform possesses green luminescence at 520 nm with excitation-independent feature. The employment of sodium borohydride during synthesis has stabilized the nanoparticle (NP) size and suppressed uncontrolled autoxidation processes. It is appealing to observe that the fluorescence as well as colorimetric titration demonstrates trace level monitoring of silver ions, and the detection limits are determined to be 68 × 10 −9 m (fluorometric) and 71 × 10 −9 m (colorimetric), respectively. The optical response to Ag + ions is assigned to a static quenching process and the corresponding redox reaction including particle aggregation may cause the "on-off " change. During in vitro studies, two living cell lines (HeLa cells and T-cells) are used and the negligible cytotoxicity of such NP is verified via flow cytometry and 3-(4,5-dimethythiazol-. 2-yl)-2,5-diphenyl tetrazolium bromide assays. More importantly, the visualization of silver recognition in two cells is realized and the availability of polydopamine-type NPs for the purpose of biomedical evaluation can generate considerable interests in future studies.

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Section: Introductionmentioning

confidence: 95%

Biocompatible Nanoplatform Based on Mussel Adhesive Chemistry: Effective Assembly, Dual Mode Sensing, and Cellular Imaging Performance

Zhang

et al. 2019

Adv Materials Inter

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“…Selective DNA detection in complex biological fluids remains challenging, because nonspecific DNA displacement by various competitive biomolecules often causes false positive signals. Inspired by the high selectivity of bioorthogonal chemistry and metal ion chelation capacity of MelNPs, metal coordination mediated DNA bioorthogonal adsorption on PDA NPs biosensor was reported ( Figure ) 145. The catechol groups on PDA NPs provided metal coordination sites for divalent calcium ions, which produced an attractive adsorption force to bridge DNA onto PDA NPs.…”

Section: Biomedical Applicationsmentioning

confidence: 99%

Section: Biomedical Applicationsmentioning

confidence: 99%

Melanin‐Like Nanomaterials for Advanced Biomedical Applications: A Versatile Platform with Extraordinary Promise

et al. 2020

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Developing efficient, sustainable, and biocompatible high‐tech nanoplatforms derived from naturally existing components in living organisms is highly beneficial for diverse advanced biomedical applications. Melanins are nontoxic natural biopolymers owning widespread distribution in various biosystems, possessing fascinating physicochemical properties and playing significant physiological roles. The multifunctionality together with intrinsic biocompatibility renders bioinspired melanin‐like nanomaterials considerably promising as a versatile and powerful nanoplatform with broad bioapplication prospects. This panoramic Review starts with an overview of the fundamental physicochemical properties, preparation methods, and polymerization mechanisms of melanins. A systematical and well‐bedded description of recent advancements of melanin‐like nanomaterials regarding diverse biomedical applications is then given, mainly focusing on biological imaging, photothermal therapy, drug delivery for tumor treatment, and other emerging biomedicine‐related implementations. Finally, current challenges toward clinical translation with an emphasis on innovative design strategies and future striving directions are rationally discussed. This comprehensive and detailed Review provides a deep understanding of the current research status of melanin‐like nanomaterials and is expected to motivate further optimization of the design of novel tailorable and marketable multifunctional nanoplatforms in biomedicine.

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“…Reprinted with permission. [95] Copyright 2018, American Chemical Society. Although in previous works (as presented in Table 1) Mg 2+ was more commonly used than Ca 2+ , Meng et al showed that at the same concentration, Ca 2+ was more effective than Mg 2+ in promoting DNA adsorption on PDA ( Figure 5C,D).…”

Section: Metal-mediated Dna Adsorptionmentioning

confidence: 99%

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

Zandieh

Hagar

Liu

2020

Part & Part Syst Charact

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and drugs, [21,22] and decrease the toxicity of biomaterials. [23] Finally, PDA can be used for sensing. PDA-containing biosensors have been used to detect various organic molecules, [24,25] biomolecules, [26,27] and metal ions. [28] Many sensing and biomedical applications of PDA have been realized through interfacing it with biomolecules such as DNA, enzymes, and antibodies. DNA has gained popularity in the last few decades as a functional polymer thanks to its high stability, low cost, and ease of synthesis. [29-34] More importantly, DNA can bind not only its complementary sequence but also a diverse range of other molecules by using DNA aptamers. [35] Catalytically active DNA sequences, known as DNAzymes, have also been discovered. [36] Combining the unique properties of PDA and DNA has produced many interesting hybrid materials. The surface of PDA is different from most inorganic and even other organic NPs, and understanding its surface interactions is critical for applications. Given the many papers on DNA-functionalized PDA which have been published, it is now a good time to summarize this topic, which was not previously reviewed. This review has two main purposes. First, it describes the interfacial interactions between DNA and PDA with a focus on methods to achieve noncovalent and covalent conjugates. Second, we review the representative applications of such conjugates. In the end, some challenges of the field are discussed, and a few future research directions are proposed. 2. Dopamine, Its Analogs, and PDA The extraordinary wet adhesion of some marine creatures, such as mussels, is caused by their secreted proteins that are rich in catecholamine groups. This has inspired scientists to develop a universal coating material that can mimic the natural wet adhesion. [37] Although dopamine is a catecholamine, PDA is not yet found naturally. PDA is a synthetic analog of melanin, and natural melanin exists in the color pigments of the skin, hair, and eyes of the human body. Melanin is produced by the aggregation and polymerization of catecholamines such as DOPA or tyrosine (Figure 1A). [38,39] To date, the polymerization pathways of dopamine are still unknown, but a few likely pathways have been proposed. [40-44] Polydopamine (PDA) is polymerized from dopamine under oxidative and basic conditions. PDA is a biocompatible material with great versatility for coating various surfaces and it can also form nanoparticles. DNA oligonucleotides are highly stable, and they can recognize a diverse range of molecules from complementary nucleic acids, proteins to small molecules and metal ions. To enhance the molecular recognition function of PDA, it is interfaced with DNA and the conjugates have achieved a wide range of applications in biomedical and analytical science. In this review, the chemistry of some catecholamines, including dopamine and PDA, is first briefly introduced and variables in the PDA synthesis are highlighted. Strategies to promote DNA adsorption on PDA are then discussed including the use of low pH and...

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Bioorthogonal DNA Adsorption on Polydopamine Nanoparticles Mediated by Metal Coordination for Highly Robust Sensing in Serum and Living Cells

Cited by 123 publications

References 69 publications

Biocompatible Nanoplatform Based on Mussel Adhesive Chemistry: Effective Assembly, Dual Mode Sensing, and Cellular Imaging Performance

Biocompatible Nanoplatform Based on Mussel Adhesive Chemistry: Effective Assembly, Dual Mode Sensing, and Cellular Imaging Performance

Melanin‐Like Nanomaterials for Advanced Biomedical Applications: A Versatile Platform with Extraordinary Promise

Interfacing DNA and Polydopamine Nanoparticles and Its Applications

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