BIOPEP-UWM Database of Bioactive Peptides: Current Opportunities

Abstract

The BIOPEP-UWM™ database of bioactive peptides (formerly BIOPEP) has recently become a popular tool in the research on bioactive peptides, especially on these derived from foods and being constituents of diets that prevent development of chronic diseases. The database is continuously updated and modified. The addition of new peptides and the introduction of new information about the existing ones (e.g., chemical codes and references to other databases) is in progress. New opportunities include the possibility of annotating peptides containing D-enantiomers of amino acids, batch processing option, converting amino acid sequences into SMILES code, new quantitative parameters characterizing the presence of bioactive fragments in protein sequences, and finding proteinases that release particular peptides.

Characteristics of Potential Protein Nutraceuticals of Plant Origin with Antioxidant Activity

“…The activity determined for this peptide was IC 50 = 63.1 µM (superoxide anion scavenging activity (SOSA)) based on the applied XTT {2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino) carbonyl]-2H-tetrazolium hydroxide}) test [43]. On the other hand, the fragments WY and LK were often found in glutenin subunits (the HMW subunits) ( Table 1), and corresponded to the peptides isolated from β-lactoglobulin (fragment [19][20][21][22][23][24][25] and from egg white protein ovotransferrin, respectively [46,60]. The activity of the WY peptide that was determined based on the ORAC was 7.67 µM Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid) equivalent/µM of peptide [60].…”

“…Since various indices are applied for the biological evaluation of proteins and given the fact that each protein may be a source of biologically active peptides, as early as two decades ago, it was proposed to introduce a new criterion for the evaluation of protein as a source of biologically active peptides. Moreover, since computer methods and tools are increasingly applied in the processing of different biological data derived from various databases and are not only applied in molecular biology and genetics but also in nutrition and food sciences, the BIOPEP-UWM (Bioactive Peptides-University of Warmia and Mazury) database (http://www.uwm.edu.pl/biochemia/index.php/ pl/biopep) has been established [20,21]. Currently, one of the four databases which make up the resources of the BIOPEP-UWM database is the "Bioactive peptides" database in which 3,820 bioactive peptide sequences have been deposited (data as at 10 December 2019) in the form of a single-letter amino acid code.…”

“…Currently, one of the four databases which make up the resources of the BIOPEP-UWM database is the "Bioactive peptides" database in which 3,820 bioactive peptide sequences have been deposited (data as at 10 December 2019) in the form of a single-letter amino acid code. In addition it contains a program that enables the construction of profiles of potential biological activity of proteins as well as the calculation of qualitative and quantitative descriptors for the assessment of the value of proteins as potential precursors of bioactive peptides, and forecasting sensitive peptide bonds for the performance of protein chain hydrolysis by proteolytic enzymes [20,21]. Currently, the BIOPEP-UWM database is commonly used in food and nutrition sciences as a source of information on biopeptides as potential nutraceuticals and components of functional food as well as compounds that serve an important role in the prevention of chronic diseases [20,[22][23][24].…”

“…In addition it contains a program that enables the construction of profiles of potential biological activity of proteins as well as the calculation of qualitative and quantitative descriptors for the assessment of the value of proteins as potential precursors of bioactive peptides, and forecasting sensitive peptide bonds for the performance of protein chain hydrolysis by proteolytic enzymes [20,21]. Currently, the BIOPEP-UWM database is commonly used in food and nutrition sciences as a source of information on biopeptides as potential nutraceuticals and components of functional food as well as compounds that serve an important role in the prevention of chronic diseases [20,[22][23][24]. Bioinformatic tools available in the BIOPEP-UWM database enable the performance of protein hydrolysis simulation with the use of proteolytic enzymes of various origins available in the database, including certain enzymes whose counterparts are synthesized in the human gastrointestinal tract or used in the food industry [8,20,25,26].…”

Peptides for Health Benefits 2019

“…Using molecular docking, Chamata et al [25] describe the structure-activity relationships of peptide sequences present in whey/milk protein hydrolysates with high angiotensin converting enzyme inhibitory activity to a better understanding and prediction of their in vivo antihypertensive activity. Minkiewicz et al [26] review the new opportunities offered by the BIOPEP-UWM database of bioactive peptides that include the possibility of annotating peptides containing D-enantiomers of amino acids, batch processing option, converting amino acid sequences into SMILES code, new quantitative parameters characterizing the presence of bioactive fragments in protein sequences and finding proteinases that release particular peptides. Using yeast proteome microarrays, Shah et al [27] identify a total of 140 and 137 intracellular protein targets of antifungal peptides of Lactoferricin B and Histatin-5, respectively.…”