Biopeptides with antioxidant activity extracted from the biomass of Spirulina sp. LEB 18

Abstract

The microalga Spirulina has been marketed in many countries due to its application in foods, especially for its high protein content, which can be used to obtain protein hydrolysates. Protein hydrolysates are easily absorbed by the gastrointestinal tract and have significant physiological properties, because during the hydrolysis process, peptides with antioxidant activity can be obtained. The objective of this study was to obtain enzymatic hydrolysates from the biomass of Spirulina sp. LEB 18 with functional properties and antioxidant potential. This study was a 2 2 type central composite design, to determine the best conditions for obtaining hydrolysates. The hydrolysis reactions of Spirulina biomass were carried out using the enzyme Protemax 580 L. The enzymatic hydrolysis of the proteins resulted in increased digestibility, solubility, water-retention capacity and antioxidant activity of hydrolysates (which increased by 1.8, 2.8 and 8.5 using the reducing power methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2' azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), respectively). Therefore, biologically active peptides of Spirulina sp. LEB 18 biomass were produced.

Uma abordagem sobre caracterização e avaliação do potencial antioxidante de extratos fenólicos de microalgas Spirulina sp. LEB-18 e Chlorella pyrenoidosa

“…De acordo com Souza (2012), os dados obtidos permitem afirmar que as microalgas estudadas são basicamente constituídas por proteínas e hidratos de carbono. Lisboa et al (2016), confirmam, em seu trabalho com hidrolisados proteico, que a biomassa de Spirulina sp. LEB 18 tem uma alta concentração de proteína e as condições requeridas para crescimento podem ser alteradas para estimular uma concentração mais elevada deste bioproduto, a concentração obtida por estes autores foi 51.66%, semelhante a este estudo (Quadro 1).…”

“…(Rodrigues, 2008;Lisboa et al, 2016). As espécies Spirulina platensis e Spirulina máxima são as mais estudadas para uso na alimentação humana por apresentarem um perfil nutricional que as torna ideais como suplemento alimentar, pois substituem satisfatoriamente as fontes artificiais de nutrientes, por combinar diversos constituintes de maneira equilibrada.…”

Bioactivity of Cyanobacterial Biomass Related to Amino Acids Induces Growth and Metabolic Changes on Seedlings and Yield Gains of Organic Red Beet

“…It is know that Cyanobacteria can stimulate plant metabolism through their bioactive compounds and thus promote plant growth [35]. Among the reported potential bioactive compounds found in these organisms, as hormones [6], polyamines [13] and amino acids [10], being the last one found at high amount in…”

“…The presence of phytohormones as auxins, gibberellins, cytokinins and abscisic acid inside this cyanobacterial cells or exogenously released by them, with potential plant growth promotion effect are being documented [9]. The A. platensis composition also presents high levels of L-amino acids [10] around 58% of their total protein content [11], which also may help in plant growth [12]. It was reported that one of the bioactive molecules on A. platensis is their polyamine content, mainly spermidine, which biosynthesis depends on L-amino acids and could stimulate cell multiplication and plant growth [13].…”

INDUSTRIAL PLANT FOR PRODUCTION OF Spirulina sp. LEB 18

“…These biocomposites contain from 3 to 20 amino acids residues and are formed from hydrolysis of the microalgal biomass as they are inactive within the protein sequence. The hydrolysis of the proteins improves the digestibility of the microalgal biomass, since molecules of lower molecular weight are obtained and are more easily absorbed by the organism (Lisboa et al, 2016).…”