Biophysical and Biochemical Changes of the Washed Buffalo Spermatozoa Added With Caffeine

Zeidan, A. E. B.; El-Harairy, M.; El-Nenaey, M.H.; El-Kishk, M. A.

doi:10.21608/kvmj.2006.112476

Cited by 2 publications

(3 citation statements)

References 14 publications

(10 reference statements)

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“…As expected, the values of ALT, AST, ALP and hyaluronidase enzymes were significantly reduced upon addition of caffeine due to the stabilizing impact of caffeine on cellular membrane (Abd Shukla andMisra, 2014). Matching with our results, addition of caffeine to the diluted cooled buffalo bull spermatozoa decreased the amounts of AST, ALT and ALP enzymes released into the extracellular medium (Zeidan et al, 2006). Activities of glutathione-S-transferases (GSTs) were decreased (P<0.01) with all caffeine samples compared with the control sample (El-Kishk, 2003 andChavda et al, 2022).…”

Section: Impact Of Seminal Plasma Removal And/or Caffeine Supplementa...supporting

confidence: 89%

“…Activities of glutathione-S-transferases (GSTs) were decreased (P<0.01) with all caffeine samples compared with the control sample (El-Kishk, 2003 andChavda et al, 2022). Results recorded that RSP reduced ALT, AST, ALP and hyaluronidase enzymes which may be related to the removal of the inhibitory factors presented in seminal plasma that causes the breakdown of sperm membrane and ease of enzymatic leakage from intracellular to extracellular fluids of spermatozoa (Sengupta et al, 1976, Zeidan et al, 2004, Bahgat, 2008and Daramola, 2017.…”

Section: Impact Of Seminal Plasma Removal And/or Caffeine Supplementa...mentioning

confidence: 98%

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Some Studies to Improve Quality and Fertilizing Ability of Cooled Buffalo Spermatozoa

El-Hawary

El-Hendawy

2023

Egyptian Journal of Nutrition and Feeds

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his work aimed to assess the influence of removal of seminal plasma (RSP) and caffeine supplementation upon quality and fertilizing ability of Egyptian buffalo-bulls' spermatozoa post 6 days of hypothermic preservation at 5ºC. Semen samples were collected from five buffalo-bulls pooled, then separated into two main parts. First part was centrifuged (washed) twice to remove the seminal plasma, then the sperm plugs were re-suspended in camel skim milk extender to a volume equal to that of semen pre-washing. Second part was non-centrifuged (unwashed). Both washed and unwashed semen were diluted (1:4) with camel skim milk extender containing 10% egg yolk supplemented with caffeine at different concentrations (0,5,10 and 15 mM) and gradually cooled to 5°C then preserved at this temperature for 6 days. Data elaborated that RSP enhanced (P<0.01) sperm motility, head-to-head agglutinated spermatozoa and conception rate percentages, while dead, abnormalities and acrosomal damage percentages of spermatozoa , as well as, leakage of ALT, AST, hyaluronidase and ALP enzymes to extracellular fluids of spermatozoa were reduced (P<0.01) post-RSP . The best (P<0.01) semen characteristics, enzymatic activities and fertilizing efficiency of buffalo-bulls' spermatozoa were registered with washed diluted buffalo-bulls semen supplemented with 10 mM caffeine. Conclusively, removal of seminal plasma is a simple, effective, and easily practicable technique, which supplies a helpful alternative to semen preserved in liquid status, especially when administrated with 10mM caffeine for optimizing quality and fertilizing efficiency of buffalo spermatozoa that can be useful for artificial insemination in Egyptian buffaloes.

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Section: Impact Of Seminal Plasma Removal And/or Caffeine Supplementa...supporting

confidence: 89%

Section: Impact Of Seminal Plasma Removal And/or Caffeine Supplementa...mentioning

confidence: 98%

Some Studies to Improve Quality and Fertilizing Ability of Cooled Buffalo Spermatozoa

El-Hawary

El-Hendawy

2023

Egyptian Journal of Nutrition and Feeds

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“…In accordance these results, Akandi et al (2015) found that propolis supplementation with a semen extender improves sperm viability and DNA integrity. In addition, extenders containing propolis were reported to lower the release of enzymes such as ALT, AST, ALP, and LDH significantly into the sperm medium (Mohamed and Zanouny, 2017;Mohamed, 2017) which may reflect the high sperm membrane integrity during incubation and storage (Zeidan et al, 2004). Improving sperm characteristics in pre-freezing and post-thaw semen was explained by several authors.…”

Section: Figurementioning

confidence: 98%

Role of Propolis Ethanolic Extract as an Antioxidant Supplement in Tris-Extender to Enhance Semen Quality of Egyptian Buffaloes during Cryopreservation

Abdel-Khalek,

Sakr,

Nagy

et al. 2023

AAVS

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This study aimed to evaluate the effect of propolis supplementation on semen extender of Egyptian buffalo semen to improve sperm characteristics and antioxidant capacity of sperm medium after cryopreservation. Tris-egg yolk extender with different levels (0, 4, 8, 12, and 14 mg/ml) of propolis ethanolic extract (PEE) were used. Semen was collected once/week for 20 weeks from five buffalo bulls aged 3.5-5 years. The collected semen was pooled and distributed into five equal fractions, each fraction was diluted with Tris-extender at each level of PEE (PEE0, PEE1, PEE2, PEE3, and PEE4). Semen was gradually diluted at 37 °C with each extender at a rate of 1: 10, equilibrated for 4 hours at 4 °C, packaged in French straws, and frozen in liquid nitrogen at -196 °C. Evaluation of semen was achieved pre-freezing in equilibrated semen and post-thawing in semen thawed in a water bath at 37 o C for 30 seconds. Results showed that PEE2 showed the highest (P < 0.05) positive impact on improving progressive motility, livability, abnormality, and acrosome integrity of spermatozoa in pre-freezing and post-thaw semen. All PEE-extenders increased (P < 0.05) viable sperm percentage, and level of total antioxidant capacity and superoxide dismutase activity, and decreased percentage of apoptosis, early apoptosis, necrosis, and malondialdehyde level; most favorable results were recorded with PEE2. The foregoing results indicated that supplementation of Tris-semen extender with propolis ethanolic extender at a level of 8 mg/ml increased the functional activity of spermatozoa, decreased sperm apoptosis, and necrosis, and improved the antioxidant status of sperm medium in cryopreserved semen of Egyptian buffaloes.

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Biophysical and Biochemical Changes of the Washed Buffalo Spermatozoa Added With Caffeine

Cited by 2 publications

References 14 publications

Some Studies to Improve Quality and Fertilizing Ability of Cooled Buffalo Spermatozoa

Some Studies to Improve Quality and Fertilizing Ability of Cooled Buffalo Spermatozoa

Role of Propolis Ethanolic Extract as an Antioxidant Supplement in Tris-Extender to Enhance Semen Quality of Egyptian Buffaloes during Cryopreservation

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