Biophysical characterization of MDR breast cancer cell lines reveals the cytoplasm is critical in determining drug sensitivity

Coley, Helen M.; Labeed, Fatima H.; Thomas, Hilary; Hughes, Michael

doi:10.1016/j.bbagen.2006.12.002

Cited by 79 publications

(65 citation statements)

References 30 publications

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“…Duncan et al [30] used the DCR method in combination with ion channel blockers to monitor changes in cytoplasmic ion levels in drug sensitive and resistant myelogenous leukemic (K562) cells. Coley et al [31] used the same technique to quantify the electrical properties of multidrug resistant breast cancer cells. They determined that the cytoplasmic conductivity is well correlated with multidrug resistant cells relative to the parent cell line.…”

Section: Measuring the Dcrmentioning

confidence: 97%

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

2011

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Over the past decade, dielectrophoresis (DEP) has evolved into a powerful, robust and flexible method for cellular characterization, manipulation, separation and cell patterning. It is a field with widely varying disciplines, as it is quite common to see DEP integrated with a host of applications including microfluidics, impedance spectroscopy, tissue engineering, real-time PCR, immunoassays, stem-cell characterization, gene transfection and electroporation, just to name a few. The field is finally at the point where analytical and numerical polarization models can be used to adequately describe and characterize the dielectrophoretic behavior of cells, and there is ever increasing evidence demonstrating that electric fields can safely be used to manipulate cells without harm. As such, DEP is slowly making its way into the biological sciences. Today, DEP is being used to manipulate individual cells to specific regions of space for single-cell assays. DEP is able to separate rare cells from a heterogeneous cell suspension, where isolated cells can then be characterized and dynamically studied using nothing more than electric fields. However, there is need for a critical report to integrate the many new features of DEP for cellular applications. Here, a review of the basic theory and current applications of DEP, specifically for cells, is presented.

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Section: Measuring the Dcrmentioning

confidence: 97%

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

2011

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“…6(D)). This identification of Table 1 Values of the parameters (Coley et al, 2007;Jen and Chen, 2009 The specific cell membrane capacitance 1.9 μF/cm 2 R c…”

Section: Resultsmentioning

confidence: 99%

Controllably moving individual living cell in an array by modulating signal phase difference based on dielectrophoresis

Guo

Zhu

2015

Biosensors and Bioelectronics

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“…Effects of thedrug were observed in 4 h with DEP, while there was no change in viability when analyzed with Trypan Blue dye in the same time span. In addition to differentiating between living and dead cells, Coley et al [66] used DEP and flow cytometry to study drug resistance of certain cell lines. Parental cell line (MCF-7) and its sublines (MDR derivatives) were analyzed under different anti-cancer drug pressures.…”

Section: Cancermentioning

confidence: 99%

Bioanalytical separations using electric field gradient techniques

2009

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The field of separations science will be strongly impacted by new electric-field-gradient-based strategies. Many new capabilities are being developed with analytical targets ranging from particles to small molecules, and soot to living cells. Here we review the emerging area of electric field gradient techniques, dividing the large variety of techniques by the target of separation. In doing so, we have contributions using dielectrophoresis, electric field gradient focusing (including dynamic, true moving bed, and pulsed field), electrocapture and electrophoretic focusing, temperature gradient focusing, and focusing with centrifugal force. We cover the literature from the start of 2007 to June 2008, along with some introductory discussions. Even with the relatively short time frame, this young and dynamic field of inquiry produced some 100 contributions describing new and unique techniques and several new applications.

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Biophysical characterization of MDR breast cancer cell lines reveals the cytoplasm is critical in determining drug sensitivity

Cited by 79 publications

References 30 publications

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

Cellular dielectrophoresis: Applications to the characterization, manipulation, separation and patterning of cells

Controllably moving individual living cell in an array by modulating signal phase difference based on dielectrophoresis

Bioanalytical separations using electric field gradient techniques

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