Controllably moving individual living cell in an array by modulating signal phase difference based on dielectrophoresis

Guo, Xiaoliang; Zhu, Rong

doi:10.1016/j.bios.2015.01.052

Cited by 33 publications

(22 citation statements)

References 28 publications

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“…Another challenge in dual-modal detection is how to position cells onto the target measuring electrodes. Cell manipulation and position have been realized using negative dielectrophoresis (nDEP) forces generated by applying electric signals onto the quadrupole-electrodes, the details of which can be found in our previous paper 23 . Before SERS detection and EIS measurement, living cells were trapped onto the measuring electrodes by nDEP forces generated from quadrupole-electrodes.…”

Section: Resultsmentioning

confidence: 99%

“…The detailed fabrication of the micro-chip can be found in our previous publication 23 . The micromachining process of the micro-chip is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…The suspended HeLa cells were poured into the sample pool of the micro-chip. The cells were manipulated and positioned at the site of the measuring electrodes using nDEP forces generated by applying electric signals onto the quadrupole-electrodes 23 . By using an appropriate concentration of cells (2 × 10 5 cells mL −1 ), single cells could be trapped onto the measureming electrodes, the detailed process of which can be found in our previous paper 23 .…”

Section: Methodsmentioning

confidence: 99%

“…We previously developed a microchip with planar quadrupole-electrodes used for single-cell trapping based on negative dielectrophoresis and a pair of planar measuring electrodes at the center of quadrupole-electrode used for single-cell impedance measurement 23 .…”

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confidence: 99%

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Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

Zong

Zhu

Guo

2015

Sci Rep

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In this paper, a fine gold nanostructure synthesized on selective planar microelectrodes in micro-chip is realized by using an advanced hybrid fabrication approach incorporating growth of nanorods (NRs) with gold electroplating. By this developed nanostructure, integration of in-situ surface-enhanced Raman spectroscopy (SERS) detection with electrochemical impedance spectroscopy (EIS) measurement for label-free, nondestructive, real-time and rapid monitoring on a single cell has been achieved. Moreover, parameters of Au nanostructures such as size of nanoholes/nanogaps can be controllably adjusted in the fabrication. We have demonstrated a SERS enhancement factor of up to ~2.24 × 106 and double-layer impedance decrease ratio of 90% ~ 95% at low frequency range below 200 kHz by using nanostructured microelectrodes. SERS detection and in-situ EIS measurement of a trapped single cell by using planar microelectrodes are realized to demonstrate the compatibility, multi-functions, high-sensitivity and simplicity of the micro-chip system. This dual function platform integrating SERS and EIS is of great significance in biological, biochemical and biomedical applications.

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Section: Resultsmentioning

confidence: 99%

“…The detailed fabrication of the micro-chip can be found in our previous publication 23 . The micromachining process of the micro-chip is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

Zong

Zhu

Guo

2015

Sci Rep

Self Cite

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“…Detailed micromachining processes to pattern the micro-array are described in [8]. The fabrication processes of microfluidic channel started with dimethylsiloxane (PDMS) prepolymer mixture (Sylgard 184 Silicone Elastomer: Sylgard 184 Curing Agent=10:1 by volume) poured into a mold and subsequently baked/cured at 60 °C for 3 hours.…”

Section: Fabrication Of Microfluidicmentioning

confidence: 99%

Characteristic parameter estimation for single-particle based on dielectrophoretic and hydrodynamic effects

Zhu

Guo

2015

2015 Transducers - 2015 18th International Conference on Solid-State Sensors, Actuators and Microsystems (TRANSDUCERS)

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Dielectrophoresis (DEP) as a well-known approach to manipulate particles can be utilized in combination with fluidics to implement a measurement on characteristic parameters of particles. Numerical simulation of DEP factors was executed together with simulation of electric field distribution by means of Comsol Multiphysics. The simulation results facilitate the establishment of dynamic equilibrium model between a DEP force and a fluid drag force. According to critical release flow-rate and signal frequency of DEP, those parameters of a particle, such as permittivity, conductivity and radius, were estimated via Levenberg Maquardt iteration algorithm. The experiments validated the method and demonstrated a sensitive and consistent measure for single-particle assay.

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Magnetophoretic Conductors and Diodes in a 3D Magnetic Field

Abedini-Nassab

Joh

Triggiano

et al. 2015

Adv Funct Materials

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We demonstrate magnetophoretic conductor tracks that can transport single magnetized beads and magnetically labeled single cells in a 3-dimensional time-varying magnetic field. The vertical field bias, in addition to the in-plane rotating field, has the advantage of reducing the attraction between particles, which inhibits the formation of particle clusters. However, the inclusion of a vertical field requires the re-design of magnetic track geometries which can transport magnetized objects across the substrate. Following insights from magnetic bubble technology, we found that successful magnetic conductor geometries defined in soft magnetic materials must be composed of alternating sections of positive and negative curvature. In addition to the previously studied magnetic tracks taken from the magnetic bubble literature, a drop-shape pattern was found to be even more adept at transporting small magnetic beads and single cells. Symmetric patterns are shown to achieve bi-directional conduction, whereas asymmetric patterns achieve unidirectional conduction. These designs represent the electrical circuit corollaries of the conductor and diode, respectively. Finally, we demonstrate biological applications in transporting single cells and in the size based separation of magnetic particles.

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Controllably moving individual living cell in an array by modulating signal phase difference based on dielectrophoresis

Cited by 33 publications

References 28 publications

Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

Nanostructured gold microelectrodes for SERS and EIS measurements by incorporating ZnO nanorod growth with electroplating

Characteristic parameter estimation for single-particle based on dielectrophoretic and hydrodynamic effects

Magnetophoretic Conductors and Diodes in a 3D Magnetic Field

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