Mn doping-induced structural and magnetic transformations in the antiferroelectric phase of the Bi1−xNdxFeO3 perovskites J. Appl. Phys. 112, 064105 (2012) Neutron diffraction study of stability and phase transitions in Cu-Sn-In alloys as alternative Pb-free solders J. Appl. Phys. 112, 053520 (2012) Communication: From graphite to diamond: Reaction pathways of the phase transition J. Chem. Phys. 137, 101101 (2012) Structural study in highly compressed BiFeO3 epitaxial thin films on YAlO3 A lead-free ceramic with the composition ͑K 0.55 Na 0.45 ͒ 0.965 Li 0.035 Nb 0.80 Ta 0.20 O 3 was found having an outstanding piezoelectric performance. It possesses high piezoelectric properties of d 33 a͒ Electronic mail: zhangjialiang@sdu.edu.cn. APPLIED PHYSICS LETTERS 95, 022909 ͑2009͒
This paper presents a novel microarray chip integrating cell positioning with in situ, real-time and long-time impedance measurement on a single cell. The microchip integrates a plurality of quadrupole-electrode units (termed positioning electrodes) patterned into an array with pairs of planar electrodes (termed measuring electrodes) located at the centers of each quadrupole-electrode unit. The positioning electrodes are utilized to trap and position living cells onto the measuring electrodes based on negative dielectrophoresis (nDEP), while the measuring electrodes are used to measure impedances of the trapped single cells. Each measuring electrode has a small footprint area of 7 × 7 μm(2) to ensure inhabiting only one single cell on it. However, the electrode with a small surface area has a low double-layer capacitance when it is immersed in a liquid solution, thus generating a large double-layer impedance, which reduces the sensitivity for impedance measurement on the single cell. To enlarge the effective surface areas of the measuring electrodes, a novel surface-modification process is proposed to controllably construct gold nanostructures on the surfaces of the measuring electrodes while the positioning electrodes are unstained. The double layer capacitances of the modified electrodes are increased by about one order after surface-modification. The developed microchip is used to monitor the adhering behavior of a single HeLa cell by measuring its impedance spectra in real time. The measured impedance is analyzed and used to extract cellular electrical parameters, which demonstrated that the cell compresses the electrical double layer in the process of adherence and adheres onto the measuring electrodes after 4-5 hours.
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