Biophysical characterization of the complexation of C<sub>60</sub> fullerene with doxorubicin in a prokaryotic model

Prylutskyy, Yu. І.; Borowik, Agnieszka; Gołuński, Grzegorz; Woziwodzka, Anna; Piosik, Jacek; Kyzyma, O. A.; Pashkova, Irina S.; Ritter, Uwe; Evstigneev, Maxim P.

doi:10.1002/mawe.201600463

Cited by 13 publications

(5 citation statements)

References 29 publications

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“…There are reports in the literature that suggest that nanostructures such as gold nanoparticles and carbon nanotubes are able to covalently interact with anticancer drugs and efficiently transport them to target cells 3,11,28,29 . On the other hand, some groups have found that nanoparticles, such as fullerene C 60 and other metal-based nanostructures, can be used as a delivery platform without interacting directly and covalently with the selected active compounds, and in consequence diminishing their biological properties 2,3,22,30,31 . To evaluate the impact of such drug-nanoparticles interactions on drugs biological activity, the bacteriological mutagenicity assay based on S. typhimurium TA98 strain can be utilized.…”

Section: Resultsmentioning

confidence: 99%

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Borowik

Banasiuk

Derewońko

et al. 2019

Sci Rep

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One of the greatest challenges of modern medicine is to find cheaper and easier ways to produce transporters for biologically active substances, which will provide selective and efficient drug delivery to the target cells, while causing low toxicity towards healthy cells. Currently, metal-based nanoparticles are considered a successful and viable solution to this problem. In this work, we propose the use of novel synthesis method of platinum nanoparticles (PtNPs) connected with their precise biophysical characterization and assessment of their potential toxicity. To work as an efficient nanodelivery platform, nanoparticles should interact with the desired active compounds spontaneously and non-covalently. We investigated possible direct interactions of PtNPs with ICR-191, a model acridine mutagen with well-established biophysical properties and mutagenic activity, by Dynamic Light Scattering, fluorescence spectroscopy, and Isothermal Titration Calorimetry. Moreover, to determine the biological activity of ICR-191-PtNPs aggregates, we employed Ames mutagenicity test, eukaryotic cell line analysis and toxicity test against the model organism Caenorhabditis elegans. PtNPs’ interesting physicochemical properties associated to the lack of toxicity in a tested range of concentrations, as well as their ability to modulate ICR-191 biological activity, suggest that these particles successfully work as potential delivery platforms for different biologically active substances.

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Section: Resultsmentioning

confidence: 99%

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Borowik

Banasiuk

Derewońko

et al. 2019

Sci Rep

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“…In AgNP-TBA and AgNP-MUA nanoparticles, oxidation and subsequent Ag + release can also be limited, because of their changed surface chemistry [2,3,8,10]. In the previous research conducted by our group, we repeatedly observed a modulating mechanism of different nanoparticles against tested mutagens and anticancer drugs [15,29,34,39,40]. Surprisingly, the naked AgNPs, AgNP-TBA, and AgNP-MUA, in general, do not modulate acridine mutagen activity.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Surface Functionalization on the Biophysical Properties of Silver Nanoparticles

et al. 2019

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Among metal-based nanoparticles, silver nanoparticles (AgNPs) are particularly appealing because of their stability, functionality, and documented antimicrobial properties. AgNPs also offer the possibility of different surface modifications. In this work, we functionalized AgNPs with thiobarbituric acid or 11-mercaptoundecanoic acid residues to improve the nanoparticles’ biological activities. Subsequently, we assessed the physicochemical properties of newly synthesized AgNPs using a wide range of biophysical methodologies, including UV/vis and fluorescence spectroscopy, atomic force and scanning electron microscopy, and dynamic light scattering and isothermal titration calorimetry. Next, we examined the effect of nanoparticles functionalization on AgNPs mutagenicity and toxicity. Our study revealed that AgNPs’ surface modification affects nanoparticles aggregation, and also impacts nanoparticles’ interaction with model acridine mutagen ICR-191. AgNPs coated with MUA showed the most interesting interactions with tested ICR-191, slightly modulating its toxicity properties by decreasing the viability in treated cells.

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“…In order to detect the complexation between C 60 fullerene and Dox in the aqueous solution, a range of physicochemical methods such as atomic force microscopy, UV–vis, nuclear magnetic resonance, and dynamic lights scattering spectroscopy were applied. , In particular, the small-angle neutron scattering data point out the existence of at least two statistically different entities in aqueous solution, which are the C 60 fullerene aggregates and C 60 –Dox nanocomplexes . Finally, within the proposed thermodynamic model of interaction between C 60 and Dox molecules, we calculated the equilibrium heterocomplexation constant, K L ≈ 60,000 M –1 .…”

Section: Methodsmentioning

confidence: 99%

“…35,36 In particular, the small-angle neutron scattering data point out the existence of at least two statistically different entities in aqueous solution, which are the C 60 fullerene aggregates and C 60 −Dox nanocomplexes. 37 Finally, within the proposed thermodynamic model of interaction between C 60 and Dox molecules, we calculated the equilibrium heterocomplexation constant, K L ≈ 60,000 M −1 . 38 Moreover, Dox release from the C 60 −Dox nanocomplex was evaluated by the fluorescent-based technique.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

et al. 2020

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Background: C 60 fullerenes and their derivatives are actively investigated for the use in neuroscience. Applications of these nanoscale materials require the examination of their interaction with different neural cells, especially with microglia, because these cells, like other tissue resident phagocytes, are the earliest and most sensitive responders to nanoparticles. The aim of this study was to investigate the effect of C 60 fullerene and its nanocomplex with doxorubicin (Dox) on the metabolic profile of brain-resident phagocytesmicrogliain vitro. Methods: Resting microglial cells from adult male Wistar rats were used in experiments. Potential C 60 fullerene targets in microglial cells were studied by computer simulation. Microglia oxidative metabolism and phagocytic activity were examined by flow cytometry. Griess reaction and arginase activity colorimetric assay were used to explore arginine metabolism. Results: C 60 fullerene when used alone did not influence microglia oxidative metabolism and phagocytic activity but shifted arginine metabolism toward the decrease of NO generation. Complexation of C 60 fullerene with Dox (C 60 −Dox) potentiated the ability of the latter to stimulate NO generation. Conclusion: The capability of C 60 fullerenes used alone to cause anti-inflammatory shift of microglia arginine metabolism makes them a promising agent for the correction of neuroinflammatory processes involved in neurodegeneration. The potentiating action of C 60 fullerene on the immunomodulatory effect of Dox allows us to consider the C 60 molecule as an attractive vehicle for this antitumor agent.

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Biophysical characterization of the complexation of C₆₀ fullerene with doxorubicin in a prokaryotic model

Cited by 13 publications

References 29 publications

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

The Impact of Surface Functionalization on the Biophysical Properties of Silver Nanoparticles

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

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Biophysical characterization of the complexation of C60 fullerene with doxorubicin in a prokaryotic model

Cited by 13 publications

References 29 publications

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

Interactions of newly synthesized platinum nanoparticles with ICR-191 and their potential application

The Impact of Surface Functionalization on the Biophysical Properties of Silver Nanoparticles

C60 Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro

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Product

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Biophysical characterization of the complexation of C₆₀ fullerene with doxorubicin in a prokaryotic model

C₆₀ Fullerene Governs Doxorubicin Effect on Metabolic Profile of Rat Microglial Cells In Vitro